Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/56256 http://dx.doi.org/10.1590/1414-431X20175163 |
Resumo: | Pseudobrickellia brasiliensis (Asteraceae) is a plant commonly known as arnica-do-campo and belongs to the native flora of the Brazilian Cerrado. The alcoholic extract of the plant has been used as an anti-inflammatory agent in folk medicine, but the biological mechanism of action has not been elucidated. The present study evaluated the composition of P. brasiliensis aqueous extract and its effects on pro-inflammatory cytokine production and lymphocyte proliferation. The extracts were prepared by sequential maceration of P. brasiliensis leaves in ethanol, ethyl acetate, and water. Extract cytotoxicity was evaluated by trypan blue exclusion assay, and apoptosis and necrosis were measured by staining with annexin V-FITC and propidium iodide. The ethanolic (ETA) and acetate (ACE) extracts showed cytotoxic effects. The aqueous extract (AQU) was not cytotoxic. Peripheral blood mononuclear cells stimulated with phorbol myristate acetate and ionomycin and treated with AQU (100 mu g/mL) showed reduced interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha expression. AQU also inhibited lymphocyte proliferative response after nonspecific stimulation with phytohemagglutinin. The aqueous extract was analyzed by liquid chromatography coupled with photodiode array detection and mass spectrometry. Quinic acid and its derivatives 5-caffeoylquinic acid and 3,5dicaffeoylquinic acid, as well as the flavonoids luteolin and luteolin dihexoside, were detected. All these compounds are known to exhibit anti-inflammatory activity. Taken together, these findings demonstrate that P. brasiliensis aqueous extract can inhibit the pro-inflammatory cytokine production and proliferative response of lymphocytes. These effects may be related to the presence of chemical substances with anti-inflammatory actions previously reported in scientific literature. |
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Almeida, V. G.Avelar-Freitas, B. A.Santos, M. G.Costa, L. A.Silva, T. J.Pereira, W. F.Amorim, M. L. L.Grael, C. F. F.Gregorio, Luiz Elídio [UNIFESP]Rocha-Vieira, E.Brito-Melo, G. E. A.2020-07-31T12:46:29Z2020-07-31T12:46:29Z2017Brazilian Journal Of Medical And Biological Research. Sao Paulo, v. 50, n. 8, p. -, 2017.0100-879Xhttps://repositorio.unifesp.br/handle/11600/56256http://dx.doi.org/10.1590/1414-431X20175163S0100-879X2017000800603.pdfS0100-879X201700080060310.1590/1414-431X20175163WOS:000405405900001Pseudobrickellia brasiliensis (Asteraceae) is a plant commonly known as arnica-do-campo and belongs to the native flora of the Brazilian Cerrado. The alcoholic extract of the plant has been used as an anti-inflammatory agent in folk medicine, but the biological mechanism of action has not been elucidated. The present study evaluated the composition of P. brasiliensis aqueous extract and its effects on pro-inflammatory cytokine production and lymphocyte proliferation. The extracts were prepared by sequential maceration of P. brasiliensis leaves in ethanol, ethyl acetate, and water. Extract cytotoxicity was evaluated by trypan blue exclusion assay, and apoptosis and necrosis were measured by staining with annexin V-FITC and propidium iodide. The ethanolic (ETA) and acetate (ACE) extracts showed cytotoxic effects. The aqueous extract (AQU) was not cytotoxic. Peripheral blood mononuclear cells stimulated with phorbol myristate acetate and ionomycin and treated with AQU (100 mu g/mL) showed reduced interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha expression. AQU also inhibited lymphocyte proliferative response after nonspecific stimulation with phytohemagglutinin. The aqueous extract was analyzed by liquid chromatography coupled with photodiode array detection and mass spectrometry. Quinic acid and its derivatives 5-caffeoylquinic acid and 3,5dicaffeoylquinic acid, as well as the flavonoids luteolin and luteolin dihexoside, were detected. All these compounds are known to exhibit anti-inflammatory activity. Taken together, these findings demonstrate that P. brasiliensis aqueous extract can inhibit the pro-inflammatory cytokine production and proliferative response of lymphocytes. These effects may be related to the presence of chemical substances with anti-inflammatory actions previously reported in scientific literature.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Vales Jequitinhonha & Mucuri, Programa Multictr Posgrad Ciencias Fisiol, Diamantina, MG, BrazilUniv Fed Vales Jequitinhonha & Mucuri, Ctr Integrado Posgrad & Pesquisa Saude Vale Jequi, Lab Imunol, Diamantina, MG, BrazilUniv Fed Vales Jequitinhonha & Mucuri, Dept Farm, Lab Farmacognosia, Diamantina, MG, BrazilUniv Fed Sao Paulo, Dept Ciencias Farmaceut, Lab Insumos Nat & Sintet, Diadema, SP, BrazilLaboratório de Insumos Naturais e Sintéticos, Departamento de Ciências Farmacêuticas, Universidade Federal de São Paulo, Diadema, SP, BrasilWeb of Science-engAssoc Bras Divulg CientificaBrazilian Journal Of Medical And Biological ResearchCytokinesLymphocyte proliferationLuteolinMedicinal plantPseudobrickellia brasiliensisQuinic acidInhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSao Paulo508info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0100-879X2017000800603.pdfapplication/pdf905924${dspace.ui.url}/bitstream/11600/56256/1/S0100-879X2017000800603.pdff84b106ccf1cdf14e540c61222a9d05aMD51open accessTEXTS0100-879X2017000800603.pdf.txtS0100-879X2017000800603.pdf.txtExtracted texttext/plain37555${dspace.ui.url}/bitstream/11600/56256/8/S0100-879X2017000800603.pdf.txtb08e384ed1acce3eb6e5df9035b52da2MD58open accessTHUMBNAILS0100-879X2017000800603.pdf.jpgS0100-879X2017000800603.pdf.jpgIM Thumbnailimage/jpeg7068${dspace.ui.url}/bitstream/11600/56256/10/S0100-879X2017000800603.pdf.jpg8254a79c0370deaf74b8ac7488ee8875MD510open access11600/562562023-06-05 19:25:08.999open accessoai:repositorio.unifesp.br:11600/56256Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:25:08Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro |
title |
Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro |
spellingShingle |
Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro Almeida, V. G. Cytokines Lymphocyte proliferation Luteolin Medicinal plant Pseudobrickellia brasiliensis Quinic acid |
title_short |
Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro |
title_full |
Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro |
title_fullStr |
Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro |
title_full_unstemmed |
Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro |
title_sort |
Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro |
author |
Almeida, V. G. |
author_facet |
Almeida, V. G. Avelar-Freitas, B. A. Santos, M. G. Costa, L. A. Silva, T. J. Pereira, W. F. Amorim, M. L. L. Grael, C. F. F. Gregorio, Luiz Elídio [UNIFESP] Rocha-Vieira, E. Brito-Melo, G. E. A. |
author_role |
author |
author2 |
Avelar-Freitas, B. A. Santos, M. G. Costa, L. A. Silva, T. J. Pereira, W. F. Amorim, M. L. L. Grael, C. F. F. Gregorio, Luiz Elídio [UNIFESP] Rocha-Vieira, E. Brito-Melo, G. E. A. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Almeida, V. G. Avelar-Freitas, B. A. Santos, M. G. Costa, L. A. Silva, T. J. Pereira, W. F. Amorim, M. L. L. Grael, C. F. F. Gregorio, Luiz Elídio [UNIFESP] Rocha-Vieira, E. Brito-Melo, G. E. A. |
dc.subject.eng.fl_str_mv |
Cytokines Lymphocyte proliferation Luteolin Medicinal plant Pseudobrickellia brasiliensis Quinic acid |
topic |
Cytokines Lymphocyte proliferation Luteolin Medicinal plant Pseudobrickellia brasiliensis Quinic acid |
description |
Pseudobrickellia brasiliensis (Asteraceae) is a plant commonly known as arnica-do-campo and belongs to the native flora of the Brazilian Cerrado. The alcoholic extract of the plant has been used as an anti-inflammatory agent in folk medicine, but the biological mechanism of action has not been elucidated. The present study evaluated the composition of P. brasiliensis aqueous extract and its effects on pro-inflammatory cytokine production and lymphocyte proliferation. The extracts were prepared by sequential maceration of P. brasiliensis leaves in ethanol, ethyl acetate, and water. Extract cytotoxicity was evaluated by trypan blue exclusion assay, and apoptosis and necrosis were measured by staining with annexin V-FITC and propidium iodide. The ethanolic (ETA) and acetate (ACE) extracts showed cytotoxic effects. The aqueous extract (AQU) was not cytotoxic. Peripheral blood mononuclear cells stimulated with phorbol myristate acetate and ionomycin and treated with AQU (100 mu g/mL) showed reduced interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha expression. AQU also inhibited lymphocyte proliferative response after nonspecific stimulation with phytohemagglutinin. The aqueous extract was analyzed by liquid chromatography coupled with photodiode array detection and mass spectrometry. Quinic acid and its derivatives 5-caffeoylquinic acid and 3,5dicaffeoylquinic acid, as well as the flavonoids luteolin and luteolin dihexoside, were detected. All these compounds are known to exhibit anti-inflammatory activity. Taken together, these findings demonstrate that P. brasiliensis aqueous extract can inhibit the pro-inflammatory cytokine production and proliferative response of lymphocytes. These effects may be related to the presence of chemical substances with anti-inflammatory actions previously reported in scientific literature. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-07-31T12:46:29Z |
dc.date.available.fl_str_mv |
2020-07-31T12:46:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal Of Medical And Biological Research. Sao Paulo, v. 50, n. 8, p. -, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/56256 http://dx.doi.org/10.1590/1414-431X20175163 |
dc.identifier.issn.none.fl_str_mv |
0100-879X |
dc.identifier.file.none.fl_str_mv |
S0100-879X2017000800603.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0100-879X2017000800603 |
dc.identifier.doi.none.fl_str_mv |
10.1590/1414-431X20175163 |
dc.identifier.wos.none.fl_str_mv |
WOS:000405405900001 |
identifier_str_mv |
Brazilian Journal Of Medical And Biological Research. Sao Paulo, v. 50, n. 8, p. -, 2017. 0100-879X S0100-879X2017000800603.pdf S0100-879X2017000800603 10.1590/1414-431X20175163 WOS:000405405900001 |
url |
https://repositorio.unifesp.br/handle/11600/56256 http://dx.doi.org/10.1590/1414-431X20175163 |
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eng |
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Brazilian Journal Of Medical And Biological Research |
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openAccess |
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Sao Paulo |
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Assoc Bras Divulg Cientifica |
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Assoc Bras Divulg Cientifica |
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