Pattern on the Antinuclear Antibody-HEp-2 Test Is a Critical Parameter for Discriminating Antinuclear Antibody-Positive Healthy Individuals and Patients With Autoimmune Rheumatic Diseases
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/33177 http://dx.doi.org/10.1002/art.30084 |
Resumo: | Objective. To identify features of antinuclear antibody (ANA)-HEp-2 test results that discriminate ANA-positive healthy individuals and patients with autoimmune rheumatic diseases (ARDs).Methods. We sequentially retrieved data on 918 healthy individuals and 153 patients with ARDs after clinical assessment. ANA-positive healthy individuals for whom data were available were reevaluated after 3.6-5.0 years. An ANA-HEp-2 test result was considered positive when a clear ANA pattern was observed at 1: 80 dilution in 2 distinct commercial HEp-2 slides by 2 blinded independent observers.Results. ANAs were present in 118 healthy individuals (12.9%) and 138 patients with ARDs (90.2%). the ANA titer was higher in patients with ARDs than in healthy individuals (P < 0.001). the ANA pattern profile was distinct in the 2 groups. Nuclear homogeneous, nuclear coarse speckled, and nuclear centromeric patterns appeared exclusively in patients with ARDs. the nuclear dense fine speckled pattern occurred only in healthy individuals. the most frequent ANA pattern in both groups was the nuclear fine speckled pattern, which occurred at lower titer in healthy individuals than in patients with ARDs (P < 0.001). Anti-extractable nuclear antigen was present in 1 healthy individual (anti-SSA/Ro) and in 52 patients with ARDs (37.7%). None of the 40 reevaluated healthy individuals developed ARDs, and 29 (72.5%) remained ANA positive. All healthy individuals who became ANA negative had an ANA titer of 1: 80 at baseline.Conclusion. Our findings suggest that the titer, and especially the pattern, on the ANA-HEp-2 test strongly enhances our ability to discriminate ANA-positive healthy individuals and patients with ARDs. |
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Mariz, Henrique de Ataíde [UNIFESP]Sato, Emilia Inoue [UNIFESP]Barbosa, Silvia Helena [UNIFESP]Rodrigues, Silvia Helena [UNIFESP]Dellavance, Alessandra [UNIFESP]Andrade, Luiz Eduardo Coelho [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:05:50Z2016-01-24T14:05:50Z2011-01-01Arthritis and Rheumatism. Hoboken: Wiley-liss, v. 63, n. 1, p. 191-200, 2011.0004-3591http://repositorio.unifesp.br/handle/11600/33177http://dx.doi.org/10.1002/art.3008410.1002/art.30084WOS:000285934100022Objective. To identify features of antinuclear antibody (ANA)-HEp-2 test results that discriminate ANA-positive healthy individuals and patients with autoimmune rheumatic diseases (ARDs).Methods. We sequentially retrieved data on 918 healthy individuals and 153 patients with ARDs after clinical assessment. ANA-positive healthy individuals for whom data were available were reevaluated after 3.6-5.0 years. An ANA-HEp-2 test result was considered positive when a clear ANA pattern was observed at 1: 80 dilution in 2 distinct commercial HEp-2 slides by 2 blinded independent observers.Results. ANAs were present in 118 healthy individuals (12.9%) and 138 patients with ARDs (90.2%). the ANA titer was higher in patients with ARDs than in healthy individuals (P < 0.001). the ANA pattern profile was distinct in the 2 groups. Nuclear homogeneous, nuclear coarse speckled, and nuclear centromeric patterns appeared exclusively in patients with ARDs. the nuclear dense fine speckled pattern occurred only in healthy individuals. the most frequent ANA pattern in both groups was the nuclear fine speckled pattern, which occurred at lower titer in healthy individuals than in patients with ARDs (P < 0.001). Anti-extractable nuclear antigen was present in 1 healthy individual (anti-SSA/Ro) and in 52 patients with ARDs (37.7%). None of the 40 reevaluated healthy individuals developed ARDs, and 29 (72.5%) remained ANA positive. All healthy individuals who became ANA negative had an ANA titer of 1: 80 at baseline.Conclusion. Our findings suggest that the titer, and especially the pattern, on the ANA-HEp-2 test strongly enhances our ability to discriminate ANA-positive healthy individuals and patients with ARDs.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brazilian Society of RheumatologyUniversidade Federal de São Paulo, Div Rheumatol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Div Rheumatol, BR-04023062 São Paulo, BrazilFAPESP: 2004/00102-9FAPESP: 04/00781-3Web of Science191-200engWiley-BlackwellArthritis and Rheumatismhttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlinfo:eu-repo/semantics/openAccessPattern on the Antinuclear Antibody-HEp-2 Test Is a Critical Parameter for Discriminating Antinuclear Antibody-Positive Healthy Individuals and Patients With Autoimmune Rheumatic Diseasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/331772022-09-27 12:00:38.783metadata only accessoai:repositorio.unifesp.br:11600/33177Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:26:31.559971Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Pattern on the Antinuclear Antibody-HEp-2 Test Is a Critical Parameter for Discriminating Antinuclear Antibody-Positive Healthy Individuals and Patients With Autoimmune Rheumatic Diseases |
title |
Pattern on the Antinuclear Antibody-HEp-2 Test Is a Critical Parameter for Discriminating Antinuclear Antibody-Positive Healthy Individuals and Patients With Autoimmune Rheumatic Diseases |
spellingShingle |
Pattern on the Antinuclear Antibody-HEp-2 Test Is a Critical Parameter for Discriminating Antinuclear Antibody-Positive Healthy Individuals and Patients With Autoimmune Rheumatic Diseases Mariz, Henrique de Ataíde [UNIFESP] |
title_short |
Pattern on the Antinuclear Antibody-HEp-2 Test Is a Critical Parameter for Discriminating Antinuclear Antibody-Positive Healthy Individuals and Patients With Autoimmune Rheumatic Diseases |
title_full |
Pattern on the Antinuclear Antibody-HEp-2 Test Is a Critical Parameter for Discriminating Antinuclear Antibody-Positive Healthy Individuals and Patients With Autoimmune Rheumatic Diseases |
title_fullStr |
Pattern on the Antinuclear Antibody-HEp-2 Test Is a Critical Parameter for Discriminating Antinuclear Antibody-Positive Healthy Individuals and Patients With Autoimmune Rheumatic Diseases |
title_full_unstemmed |
Pattern on the Antinuclear Antibody-HEp-2 Test Is a Critical Parameter for Discriminating Antinuclear Antibody-Positive Healthy Individuals and Patients With Autoimmune Rheumatic Diseases |
title_sort |
Pattern on the Antinuclear Antibody-HEp-2 Test Is a Critical Parameter for Discriminating Antinuclear Antibody-Positive Healthy Individuals and Patients With Autoimmune Rheumatic Diseases |
author |
Mariz, Henrique de Ataíde [UNIFESP] |
author_facet |
Mariz, Henrique de Ataíde [UNIFESP] Sato, Emilia Inoue [UNIFESP] Barbosa, Silvia Helena [UNIFESP] Rodrigues, Silvia Helena [UNIFESP] Dellavance, Alessandra [UNIFESP] Andrade, Luiz Eduardo Coelho [UNIFESP] |
author_role |
author |
author2 |
Sato, Emilia Inoue [UNIFESP] Barbosa, Silvia Helena [UNIFESP] Rodrigues, Silvia Helena [UNIFESP] Dellavance, Alessandra [UNIFESP] Andrade, Luiz Eduardo Coelho [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Mariz, Henrique de Ataíde [UNIFESP] Sato, Emilia Inoue [UNIFESP] Barbosa, Silvia Helena [UNIFESP] Rodrigues, Silvia Helena [UNIFESP] Dellavance, Alessandra [UNIFESP] Andrade, Luiz Eduardo Coelho [UNIFESP] |
description |
Objective. To identify features of antinuclear antibody (ANA)-HEp-2 test results that discriminate ANA-positive healthy individuals and patients with autoimmune rheumatic diseases (ARDs).Methods. We sequentially retrieved data on 918 healthy individuals and 153 patients with ARDs after clinical assessment. ANA-positive healthy individuals for whom data were available were reevaluated after 3.6-5.0 years. An ANA-HEp-2 test result was considered positive when a clear ANA pattern was observed at 1: 80 dilution in 2 distinct commercial HEp-2 slides by 2 blinded independent observers.Results. ANAs were present in 118 healthy individuals (12.9%) and 138 patients with ARDs (90.2%). the ANA titer was higher in patients with ARDs than in healthy individuals (P < 0.001). the ANA pattern profile was distinct in the 2 groups. Nuclear homogeneous, nuclear coarse speckled, and nuclear centromeric patterns appeared exclusively in patients with ARDs. the nuclear dense fine speckled pattern occurred only in healthy individuals. the most frequent ANA pattern in both groups was the nuclear fine speckled pattern, which occurred at lower titer in healthy individuals than in patients with ARDs (P < 0.001). Anti-extractable nuclear antigen was present in 1 healthy individual (anti-SSA/Ro) and in 52 patients with ARDs (37.7%). None of the 40 reevaluated healthy individuals developed ARDs, and 29 (72.5%) remained ANA positive. All healthy individuals who became ANA negative had an ANA titer of 1: 80 at baseline.Conclusion. Our findings suggest that the titer, and especially the pattern, on the ANA-HEp-2 test strongly enhances our ability to discriminate ANA-positive healthy individuals and patients with ARDs. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-01-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:05:50Z |
dc.date.available.fl_str_mv |
2016-01-24T14:05:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Arthritis and Rheumatism. Hoboken: Wiley-liss, v. 63, n. 1, p. 191-200, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/33177 http://dx.doi.org/10.1002/art.30084 |
dc.identifier.issn.none.fl_str_mv |
0004-3591 |
dc.identifier.doi.none.fl_str_mv |
10.1002/art.30084 |
dc.identifier.wos.none.fl_str_mv |
WOS:000285934100022 |
identifier_str_mv |
Arthritis and Rheumatism. Hoboken: Wiley-liss, v. 63, n. 1, p. 191-200, 2011. 0004-3591 10.1002/art.30084 WOS:000285934100022 |
url |
http://repositorio.unifesp.br/handle/11600/33177 http://dx.doi.org/10.1002/art.30084 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Arthritis and Rheumatism |
dc.rights.driver.fl_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
191-200 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1783460291230564352 |