Interaction between saliva's adenosine and tick parasitism: effects on feeding and reproduction
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1186/s13071-017-2248-8 https://repositorio.unifesp.br/handle/11600/53456 |
Resumo: | Background: It has recently been demonstrated that saliva from Rhipicephalus sanguineus ticks contains adenosine (ADO) and prostaglandin E2 (PGE2), two non-protein molecules that have significant immunomodulatory properties. These molecules can inhibit cytokine production by dendritic cells (DCs), while also reducing the expression of CD40 in these cells. However, more studies are needed for a better understanding of their participation in the feeding of ticks in vivo. This work, therefore, evaluated the importance of ADO during tick infestations. Mice were infested with adult ticks (3 couples/mouse), and their skin was collected at the tick-infested site (3rd and 7th day), and mRNA for receptors of ADO was quantified by real-time PCR. Results: Tick infestation increased by four and two times the expression of the A2b and A3v1 receptors on day 3, respectively, while expression of other ADO receptors was unaltered. In addition, we treated mice (n = 10/group) daily with 8-(p-Sulfophenyl) theophylline, 8-pSPT, 20 mg/kg, i.p.), a non-selective antagonist of ADO receptors, and evaluated the performance of ticks during infestations. Female ticks fed on 8-pSPT-treated mice presented a reduction in their engorgement, weight and hatching rates of egg masses, and survival times of larvae compared to the same parameters presented by ticks in the control group. To investigate if these 8-pSPT-treated mice presented altered immune responses, we performed three tick infestations and collected their lymph node cells to determine the percentages and activation state of DCs and cytokine production by lymphocytes by flow cytometry (Cytometric Bead Array technique, CBA). Our data showed that 8-pSPT-treated mice presented an increase in the percentage of DCs as well as of their stimulatory and co-stimulatory molecules (CD40, CD80 and MHCII). Regarding production of T cell cytokines, we observed a significant increase in the levels of IL-2 and a significant decrease in IL-10, IL-17, TNF-alpha and IFN-gamma cytokines. Conclusions: These results suggest that ADO produced by ticks helps them feed and reproduce and that this effect may be due to modulation of host DCs and T cells. |
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Anatriello, Elen [UNIFESP]Freire Oliveira, Carlo JoseOliveira, Nathalia BaptistaFisch, AndressaMilanezi, Cristiane Mariada Silva, Joao SantanaFerreira de Miranda-Santos, Isabel KinneyFerreira, Beatriz Rossetti2020-06-26T16:30:16Z2020-06-26T16:30:16Z2017http://dx.doi.org/10.1186/s13071-017-2248-8Parasites & Vectors. London, v. 10, p. -, 2017.1756-3305https://repositorio.unifesp.br/handle/11600/53456WOS000405902200001.pdf10.1186/s13071-017-2248-8WOS:000405902200001Background: It has recently been demonstrated that saliva from Rhipicephalus sanguineus ticks contains adenosine (ADO) and prostaglandin E2 (PGE2), two non-protein molecules that have significant immunomodulatory properties. These molecules can inhibit cytokine production by dendritic cells (DCs), while also reducing the expression of CD40 in these cells. However, more studies are needed for a better understanding of their participation in the feeding of ticks in vivo. This work, therefore, evaluated the importance of ADO during tick infestations. Mice were infested with adult ticks (3 couples/mouse), and their skin was collected at the tick-infested site (3rd and 7th day), and mRNA for receptors of ADO was quantified by real-time PCR. Results: Tick infestation increased by four and two times the expression of the A2b and A3v1 receptors on day 3, respectively, while expression of other ADO receptors was unaltered. In addition, we treated mice (n = 10/group) daily with 8-(p-Sulfophenyl) theophylline, 8-pSPT, 20 mg/kg, i.p.), a non-selective antagonist of ADO receptors, and evaluated the performance of ticks during infestations. Female ticks fed on 8-pSPT-treated mice presented a reduction in their engorgement, weight and hatching rates of egg masses, and survival times of larvae compared to the same parameters presented by ticks in the control group. To investigate if these 8-pSPT-treated mice presented altered immune responses, we performed three tick infestations and collected their lymph node cells to determine the percentages and activation state of DCs and cytokine production by lymphocytes by flow cytometry (Cytometric Bead Array technique, CBA). Our data showed that 8-pSPT-treated mice presented an increase in the percentage of DCs as well as of their stimulatory and co-stimulatory molecules (CD40, CD80 and MHCII). Regarding production of T cell cytokines, we observed a significant increase in the levels of IL-2 and a significant decrease in IL-10, IL-17, TNF-alpha and IFN-gamma cytokines. Conclusions: These results suggest that ADO produced by ticks helps them feed and reproduce and that this effect may be due to modulation of host DCs and T cells.Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Sao Paulo Research Foundation (FAPESP)Univ Fed Sao Paulo, UNIFESP, Inst Sci & Technol, Rua Talim 330, BR-12231280 Sao Jose Dos Campos, SP, BrazilUniv Fed Triangulo Mineiro, UFTM, Inst Biol & Nat Sci, Praca Manoel Terra 330, BR-38015050 Uberaba, MG, BrazilUniv Sao Paulo, Ribeirao Preto Sch Nursing, Dept Maternal & Child & Publ Hlth Nursing, Ave Bandeirantes 3900, BR-14040902 Ribeirao Preto, SP, BrazilUniv Sao Paulo, Ribeirao Preto Sch Nursing, Dept Biochem & Immunol, Ave Bandeirantes 3900, BR-14040900 Ribeirao Preto, SP, BrazilUniv Fed Sao Paulo, UNIFESP, Inst Sci & Technol, Rua Talim 330, BR-12231280 Sao Jose Dos Campos, SP, BrazilCNPq: 301,663/2007-6CNPq: 308,815/2010-6CNPq: 308,280/2013-0FAPESP: 2011/00905-8FAPESP: 2010/11285-8Web of Science-engBiomed Central LtdParasites & VectorsTicksRhipicephalus sanguineusSalivaAdenosineDendritic cellsT cellsInteraction between saliva's adenosine and tick parasitism: effects on feeding and reproductioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLondon10info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000405902200001.pdfapplication/pdf1688860${dspace.ui.url}/bitstream/11600/53456/1/WOS000405902200001.pdfe765af22e0c7a67b48afc2ad2fce841dMD51open accessTEXTWOS000405902200001.pdf.txtWOS000405902200001.pdf.txtExtracted texttext/plain53858${dspace.ui.url}/bitstream/11600/53456/8/WOS000405902200001.pdf.txtb48946f3acca60275cad4260ec0bbee6MD58open accessTHUMBNAILWOS000405902200001.pdf.jpgWOS000405902200001.pdf.jpgIM Thumbnailimage/jpeg7164${dspace.ui.url}/bitstream/11600/53456/10/WOS000405902200001.pdf.jpgcd188af2732c06d89d9b66de7e479597MD510open access11600/534562023-06-05 19:09:21.097open accessoai:repositorio.unifesp.br:11600/53456Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:09:21Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Interaction between saliva's adenosine and tick parasitism: effects on feeding and reproduction |
title |
Interaction between saliva's adenosine and tick parasitism: effects on feeding and reproduction |
spellingShingle |
Interaction between saliva's adenosine and tick parasitism: effects on feeding and reproduction Anatriello, Elen [UNIFESP] Ticks Rhipicephalus sanguineus Saliva Adenosine Dendritic cells T cells |
title_short |
Interaction between saliva's adenosine and tick parasitism: effects on feeding and reproduction |
title_full |
Interaction between saliva's adenosine and tick parasitism: effects on feeding and reproduction |
title_fullStr |
Interaction between saliva's adenosine and tick parasitism: effects on feeding and reproduction |
title_full_unstemmed |
Interaction between saliva's adenosine and tick parasitism: effects on feeding and reproduction |
title_sort |
Interaction between saliva's adenosine and tick parasitism: effects on feeding and reproduction |
author |
Anatriello, Elen [UNIFESP] |
author_facet |
Anatriello, Elen [UNIFESP] Freire Oliveira, Carlo Jose Oliveira, Nathalia Baptista Fisch, Andressa Milanezi, Cristiane Maria da Silva, Joao Santana Ferreira de Miranda-Santos, Isabel Kinney Ferreira, Beatriz Rossetti |
author_role |
author |
author2 |
Freire Oliveira, Carlo Jose Oliveira, Nathalia Baptista Fisch, Andressa Milanezi, Cristiane Maria da Silva, Joao Santana Ferreira de Miranda-Santos, Isabel Kinney Ferreira, Beatriz Rossetti |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Anatriello, Elen [UNIFESP] Freire Oliveira, Carlo Jose Oliveira, Nathalia Baptista Fisch, Andressa Milanezi, Cristiane Maria da Silva, Joao Santana Ferreira de Miranda-Santos, Isabel Kinney Ferreira, Beatriz Rossetti |
dc.subject.eng.fl_str_mv |
Ticks Rhipicephalus sanguineus Saliva Adenosine Dendritic cells T cells |
topic |
Ticks Rhipicephalus sanguineus Saliva Adenosine Dendritic cells T cells |
description |
Background: It has recently been demonstrated that saliva from Rhipicephalus sanguineus ticks contains adenosine (ADO) and prostaglandin E2 (PGE2), two non-protein molecules that have significant immunomodulatory properties. These molecules can inhibit cytokine production by dendritic cells (DCs), while also reducing the expression of CD40 in these cells. However, more studies are needed for a better understanding of their participation in the feeding of ticks in vivo. This work, therefore, evaluated the importance of ADO during tick infestations. Mice were infested with adult ticks (3 couples/mouse), and their skin was collected at the tick-infested site (3rd and 7th day), and mRNA for receptors of ADO was quantified by real-time PCR. Results: Tick infestation increased by four and two times the expression of the A2b and A3v1 receptors on day 3, respectively, while expression of other ADO receptors was unaltered. In addition, we treated mice (n = 10/group) daily with 8-(p-Sulfophenyl) theophylline, 8-pSPT, 20 mg/kg, i.p.), a non-selective antagonist of ADO receptors, and evaluated the performance of ticks during infestations. Female ticks fed on 8-pSPT-treated mice presented a reduction in their engorgement, weight and hatching rates of egg masses, and survival times of larvae compared to the same parameters presented by ticks in the control group. To investigate if these 8-pSPT-treated mice presented altered immune responses, we performed three tick infestations and collected their lymph node cells to determine the percentages and activation state of DCs and cytokine production by lymphocytes by flow cytometry (Cytometric Bead Array technique, CBA). Our data showed that 8-pSPT-treated mice presented an increase in the percentage of DCs as well as of their stimulatory and co-stimulatory molecules (CD40, CD80 and MHCII). Regarding production of T cell cytokines, we observed a significant increase in the levels of IL-2 and a significant decrease in IL-10, IL-17, TNF-alpha and IFN-gamma cytokines. Conclusions: These results suggest that ADO produced by ticks helps them feed and reproduce and that this effect may be due to modulation of host DCs and T cells. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-06-26T16:30:16Z |
dc.date.available.fl_str_mv |
2020-06-26T16:30:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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dc.identifier.].fl_str_mv |
http://dx.doi.org/10.1186/s13071-017-2248-8 |
dc.identifier.citation.fl_str_mv |
Parasites & Vectors. London, v. 10, p. -, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/53456 |
dc.identifier.issn.none.fl_str_mv |
1756-3305 |
dc.identifier.file.none.fl_str_mv |
WOS000405902200001.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1186/s13071-017-2248-8 |
dc.identifier.wos.none.fl_str_mv |
WOS:000405902200001 |
url |
http://dx.doi.org/10.1186/s13071-017-2248-8 https://repositorio.unifesp.br/handle/11600/53456 |
identifier_str_mv |
Parasites & Vectors. London, v. 10, p. -, 2017. 1756-3305 WOS000405902200001.pdf 10.1186/s13071-017-2248-8 WOS:000405902200001 |
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eng |
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Parasites & Vectors |
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openAccess |
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- |
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London |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
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