Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum

Detalhes bibliográficos
Autor(a) principal: Cordeiro, R. de Aguiar
Data de Publicação: 2007
Outros Autores: Nogueira Brilhante, R. S., Gadelha Rocha, M. F., Araujo Moura, F. E., Camargo, Zoilo Pires de [UNIFESP], Costa Sidrim, J. J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/29605
http://dx.doi.org/10.1111/j.1469-0691.2006.01679.x
Resumo: Coccidioidomycosis is a deep infection caused by two dimorphic fungi, Coccidioides immitis and Coccidioides posadasii. Diagnosis of the disease requires culture of suspicious clinical samples on mycological media. However, as these species are virulent pathogens, handling of their cultures is a high-risk activity, and is limited to Biosafety Level 3 laboratories. This study describes the direct detection of C. posadasii DNA in an inappropriate sputum sample by PCR amplification of the highly specific Ag2/PRA antigen gene. the results obtained suggest that direct detection of the Ag2/PRA sequence in sputum is an excellent method for rapid and specific diagnosis of coccidioidomycosis.
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spelling Cordeiro, R. de AguiarNogueira Brilhante, R. S.Gadelha Rocha, M. F.Araujo Moura, F. E.Camargo, Zoilo Pires de [UNIFESP]Costa Sidrim, J. J.Fed Univ CearaState Univ CearaUniversidade Federal de São Paulo (UNIFESP)2016-01-24T12:42:00Z2016-01-24T12:42:00Z2007-04-01Clinical Microbiology and Infection. Oxford: Blackwell Publishing, v. 13, n. 4, p. 449-451, 2007.1198-743Xhttp://repositorio.unifesp.br/handle/11600/29605http://dx.doi.org/10.1111/j.1469-0691.2006.01679.xWOS000244718200019.pdf10.1111/j.1469-0691.2006.01679.xWOS:000244718200019Coccidioidomycosis is a deep infection caused by two dimorphic fungi, Coccidioides immitis and Coccidioides posadasii. Diagnosis of the disease requires culture of suspicious clinical samples on mycological media. However, as these species are virulent pathogens, handling of their cultures is a high-risk activity, and is limited to Biosafety Level 3 laboratories. This study describes the direct detection of C. posadasii DNA in an inappropriate sputum sample by PCR amplification of the highly specific Ag2/PRA antigen gene. the results obtained suggest that direct detection of the Ag2/PRA sequence in sputum is an excellent method for rapid and specific diagnosis of coccidioidomycosis.Fed Univ Ceara, Med Mycol Specialized Ctr, Fortaleza, Ceara, BrazilFed Univ Ceara, Postgrad Program Med Sci, Fortaleza, Ceara, BrazilState Univ Ceara, Postgrad Program Vet Sci, Fortaleza, Ceara, BrazilState Univ Ceara, Dept Sci Biol, Fortaleza, Ceara, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilWeb of Science449-451engBlackwell PublishingClinical Microbiology and InfectionAg2/PRA genecoccidioidomycosisDNA extractionmolecular diagnosisPCRsputumRapid diagnosis of coccidioidomycosis by nested PCR assay of sputuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000244718200019.pdfapplication/pdf207720${dspace.ui.url}/bitstream/11600/29605/1/WOS000244718200019.pdf08eefc2829e1bf038f01c24083f664adMD51open accessTEXTWOS000244718200019.pdf.txtWOS000244718200019.pdf.txtExtracted texttext/plain11810${dspace.ui.url}/bitstream/11600/29605/2/WOS000244718200019.pdf.txt7b1f34d87161b584e3112d151ceb4376MD52open access11600/296052022-09-27 14:37:27.723open accessoai:repositorio.unifesp.br:11600/29605Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:26:50.624421Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum
title Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum
spellingShingle Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum
Cordeiro, R. de Aguiar
Ag2/PRA gene
coccidioidomycosis
DNA extraction
molecular diagnosis
PCR
sputum
title_short Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum
title_full Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum
title_fullStr Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum
title_full_unstemmed Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum
title_sort Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum
author Cordeiro, R. de Aguiar
author_facet Cordeiro, R. de Aguiar
Nogueira Brilhante, R. S.
Gadelha Rocha, M. F.
Araujo Moura, F. E.
Camargo, Zoilo Pires de [UNIFESP]
Costa Sidrim, J. J.
author_role author
author2 Nogueira Brilhante, R. S.
Gadelha Rocha, M. F.
Araujo Moura, F. E.
Camargo, Zoilo Pires de [UNIFESP]
Costa Sidrim, J. J.
author2_role author
author
author
author
author
dc.contributor.institution.none.fl_str_mv Fed Univ Ceara
State Univ Ceara
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Cordeiro, R. de Aguiar
Nogueira Brilhante, R. S.
Gadelha Rocha, M. F.
Araujo Moura, F. E.
Camargo, Zoilo Pires de [UNIFESP]
Costa Sidrim, J. J.
dc.subject.eng.fl_str_mv Ag2/PRA gene
coccidioidomycosis
DNA extraction
molecular diagnosis
PCR
sputum
topic Ag2/PRA gene
coccidioidomycosis
DNA extraction
molecular diagnosis
PCR
sputum
description Coccidioidomycosis is a deep infection caused by two dimorphic fungi, Coccidioides immitis and Coccidioides posadasii. Diagnosis of the disease requires culture of suspicious clinical samples on mycological media. However, as these species are virulent pathogens, handling of their cultures is a high-risk activity, and is limited to Biosafety Level 3 laboratories. This study describes the direct detection of C. posadasii DNA in an inappropriate sputum sample by PCR amplification of the highly specific Ag2/PRA antigen gene. the results obtained suggest that direct detection of the Ag2/PRA sequence in sputum is an excellent method for rapid and specific diagnosis of coccidioidomycosis.
publishDate 2007
dc.date.issued.fl_str_mv 2007-04-01
dc.date.accessioned.fl_str_mv 2016-01-24T12:42:00Z
dc.date.available.fl_str_mv 2016-01-24T12:42:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Clinical Microbiology and Infection. Oxford: Blackwell Publishing, v. 13, n. 4, p. 449-451, 2007.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/29605
http://dx.doi.org/10.1111/j.1469-0691.2006.01679.x
dc.identifier.issn.none.fl_str_mv 1198-743X
dc.identifier.file.none.fl_str_mv WOS000244718200019.pdf
dc.identifier.doi.none.fl_str_mv 10.1111/j.1469-0691.2006.01679.x
dc.identifier.wos.none.fl_str_mv WOS:000244718200019
identifier_str_mv Clinical Microbiology and Infection. Oxford: Blackwell Publishing, v. 13, n. 4, p. 449-451, 2007.
1198-743X
WOS000244718200019.pdf
10.1111/j.1469-0691.2006.01679.x
WOS:000244718200019
url http://repositorio.unifesp.br/handle/11600/29605
http://dx.doi.org/10.1111/j.1469-0691.2006.01679.x
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Clinical Microbiology and Infection
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 449-451
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
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