Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/33385 http://dx.doi.org/10.1016/j.resmic.2010.09.022 |
Resumo: | Antimicrobial resistance patterns and molecular characteristics were determined in thirty-two Shiga-toxin-producing Escherichia coli (STEC) strains previously identified in São Paulo State associated with human infections (n = 21) and in cattle feces (n = 11). the highest resistance rates were identified for tetracycline (100%), streptomycin (78%) and trimethoprim sulfamethoxazole (56%). Eleven STEC strains showed resistance to ampicillin and carried bla(TEM) that was confirmed as bla(TEM-1) in one representative isolate. the class 1 integrase gene (intI1) was detected in seven (22%) strains, and most of them belonged to the O111:H8 serotype. the class 1 integron was located on plasmids in five of the seven STEC strains, and conjugation assays confirmed the plasmid support of those resistant determinants. STEC strains were genetically classified into the B I group, and PFGE analysis showed that most of the strains in each serogroup were grouped into the same cluster (80-97% similarity). the presence of a class 1 integron and bla(TEM-1) genes is described for the first time among STEC isolates in Brazil and clearly represents a public health concern. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. |
id |
UFSP_71287aac768d94136d0c203c76912856 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/33385 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Cergole-Novella, Maria Cecilia [UNIFESP]Pignatari, Antonio Carlos Campos [UNIFESP]Castanheira, Mariana [UNIFESP]Guth, Beatriz Ernestina Cabilio [UNIFESP]Universidade Federal de São Paulo (UNIFESP)JMI Labs2016-01-24T14:06:05Z2016-01-24T14:06:05Z2011-02-01Research in Microbiology. Amsterdam: Elsevier B.V., v. 162, n. 2, p. 117-123, 2011.0923-2508http://repositorio.unifesp.br/handle/11600/33385http://dx.doi.org/10.1016/j.resmic.2010.09.022WOS000288880500003.pdf10.1016/j.resmic.2010.09.022WOS:000288880500003Antimicrobial resistance patterns and molecular characteristics were determined in thirty-two Shiga-toxin-producing Escherichia coli (STEC) strains previously identified in São Paulo State associated with human infections (n = 21) and in cattle feces (n = 11). the highest resistance rates were identified for tetracycline (100%), streptomycin (78%) and trimethoprim sulfamethoxazole (56%). Eleven STEC strains showed resistance to ampicillin and carried bla(TEM) that was confirmed as bla(TEM-1) in one representative isolate. the class 1 integrase gene (intI1) was detected in seven (22%) strains, and most of them belonged to the O111:H8 serotype. the class 1 integron was located on plasmids in five of the seven STEC strains, and conjugation assays confirmed the plasmid support of those resistant determinants. STEC strains were genetically classified into the B I group, and PFGE analysis showed that most of the strains in each serogroup were grouped into the same cluster (80-97% similarity). the presence of a class 1 integron and bla(TEM-1) genes is described for the first time among STEC isolates in Brazil and clearly represents a public health concern. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Div Infect Dis, BR-04023062 São Paulo, BrazilJMI Labs, N Liberty, IA USAUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Div Infect Dis, BR-04023062 São Paulo, BrazilWeb of Science117-123engElsevier B.V.Research in Microbiologyhttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessShiga-toxin-producing Escherichia coliResistance genesClass 1 integronPhylogenetic backgroundMolecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000288880500003.pdfapplication/pdf703605${dspace.ui.url}/bitstream/11600/33385/1/WOS000288880500003.pdfc923411d7b8fc0f9a527d4877298885dMD51open accessTEXTWOS000288880500003.pdf.txtWOS000288880500003.pdf.txtExtracted texttext/plain30015${dspace.ui.url}/bitstream/11600/33385/2/WOS000288880500003.pdf.txt9bcdcaab2b451c847962e33c2726c255MD52open access11600/333852022-11-04 14:18:42.746open accessoai:repositorio.unifesp.br:11600/33385Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:28:31.027531Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazil |
title |
Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazil |
spellingShingle |
Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazil Cergole-Novella, Maria Cecilia [UNIFESP] Shiga-toxin-producing Escherichia coli Resistance genes Class 1 integron Phylogenetic background |
title_short |
Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazil |
title_full |
Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazil |
title_fullStr |
Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazil |
title_full_unstemmed |
Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazil |
title_sort |
Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazil |
author |
Cergole-Novella, Maria Cecilia [UNIFESP] |
author_facet |
Cergole-Novella, Maria Cecilia [UNIFESP] Pignatari, Antonio Carlos Campos [UNIFESP] Castanheira, Mariana [UNIFESP] Guth, Beatriz Ernestina Cabilio [UNIFESP] |
author_role |
author |
author2 |
Pignatari, Antonio Carlos Campos [UNIFESP] Castanheira, Mariana [UNIFESP] Guth, Beatriz Ernestina Cabilio [UNIFESP] |
author2_role |
author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) JMI Labs |
dc.contributor.author.fl_str_mv |
Cergole-Novella, Maria Cecilia [UNIFESP] Pignatari, Antonio Carlos Campos [UNIFESP] Castanheira, Mariana [UNIFESP] Guth, Beatriz Ernestina Cabilio [UNIFESP] |
dc.subject.eng.fl_str_mv |
Shiga-toxin-producing Escherichia coli Resistance genes Class 1 integron Phylogenetic background |
topic |
Shiga-toxin-producing Escherichia coli Resistance genes Class 1 integron Phylogenetic background |
description |
Antimicrobial resistance patterns and molecular characteristics were determined in thirty-two Shiga-toxin-producing Escherichia coli (STEC) strains previously identified in São Paulo State associated with human infections (n = 21) and in cattle feces (n = 11). the highest resistance rates were identified for tetracycline (100%), streptomycin (78%) and trimethoprim sulfamethoxazole (56%). Eleven STEC strains showed resistance to ampicillin and carried bla(TEM) that was confirmed as bla(TEM-1) in one representative isolate. the class 1 integrase gene (intI1) was detected in seven (22%) strains, and most of them belonged to the O111:H8 serotype. the class 1 integron was located on plasmids in five of the seven STEC strains, and conjugation assays confirmed the plasmid support of those resistant determinants. STEC strains were genetically classified into the B I group, and PFGE analysis showed that most of the strains in each serogroup were grouped into the same cluster (80-97% similarity). the presence of a class 1 integron and bla(TEM-1) genes is described for the first time among STEC isolates in Brazil and clearly represents a public health concern. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-02-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:06:05Z |
dc.date.available.fl_str_mv |
2016-01-24T14:06:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Research in Microbiology. Amsterdam: Elsevier B.V., v. 162, n. 2, p. 117-123, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/33385 http://dx.doi.org/10.1016/j.resmic.2010.09.022 |
dc.identifier.issn.none.fl_str_mv |
0923-2508 |
dc.identifier.file.none.fl_str_mv |
WOS000288880500003.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.resmic.2010.09.022 |
dc.identifier.wos.none.fl_str_mv |
WOS:000288880500003 |
identifier_str_mv |
Research in Microbiology. Amsterdam: Elsevier B.V., v. 162, n. 2, p. 117-123, 2011. 0923-2508 WOS000288880500003.pdf 10.1016/j.resmic.2010.09.022 WOS:000288880500003 |
url |
http://repositorio.unifesp.br/handle/11600/33385 http://dx.doi.org/10.1016/j.resmic.2010.09.022 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Research in Microbiology |
dc.rights.driver.fl_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
117-123 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/33385/1/WOS000288880500003.pdf ${dspace.ui.url}/bitstream/11600/33385/2/WOS000288880500003.pdf.txt |
bitstream.checksum.fl_str_mv |
c923411d7b8fc0f9a527d4877298885d 9bcdcaab2b451c847962e33c2726c255 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1783460295728955392 |