Autoantibodies to DEK oncoprotein in human inflammatory disease

• Autor(a) principal: Dong, X. W.
• Data de Publicação: 2000
• Outros Autores: Wang, J. S., Kabir, F. N., Shaw, M., Reed, A. M., Stein, L., Andrade, Luiz Eduardo Coelho [UNIFESP], Trevisani, Virgínia Fernandes Moça [UNIFESP], Miller, M. L., Fujii, T., Akizuki, M., Pachman, L. M., Satoh, M., Reeves, W. H.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://repositorio.unifesp.br/handle/11600/26198; http://dx.doi.org/10.1002/1529-0131(200001)43:1<85

Objective. To evaluate the specificity of anti-DEK antibodies for juvenile rheumatoid arthritis (JRA),Methods. Anti-DEK autoantibodies were measured by enzyme-linked immunosorbent assay (ELISA) using affinity-purified his6-DEK fusion protein. Sera from 639 subjects (417 patients with systemic autoimmune disease, 13 with sarcoidosis, 44 with pulmonary tuberculosis, 125 with uveitis, and 6 with scleritis, and 34 healthy control subjects) were screened. Reactivity was verified by immunoblotting and immunoprecipitation Studies using baculovirus-expressed human DEK.Results. Anti-DEK activity was found at the following frequencies: JRA 39.4% (n = 71), systemic lupus erythematosus (SLE) 25.1% (n = 216), sarcoidosis 46.2% (n = 13), rheumatoid arthritis 15.5% (n = 71), systemic sclerosis 36.0% (n = 22), polymyositis 6.2% (n = 16), and adult Still's disease 0% (n = 21), Autoantibodies also were detected in 9.1% of tuberculosis sera (n = 44), but were undetectable in sera from the 34 healthy controls. Western blot and immunoprecipitation assay results correlated well with the ELISA findings. in general, levels of anti-DEK autoantibodies were higher in SLE than in other patient subsets, including JRA.Conclusion. Anti-DEK autoantibodies are less specific for JRA than previously believed. They are produced in association with a variety of inflammatory conditions, many of which are associated with granuloma formation and/or predominant Th1 cytokine production. Anti-DEK antibodies may be a marker for a subset of autoimmunity associated with interferon-gamma production rather than a particular disease subset.