Autoantibodies to DEK oncoprotein in human inflammatory disease
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/26198 http://dx.doi.org/10.1002/1529-0131(200001)43:1<85 |
Resumo: | Objective. To evaluate the specificity of anti-DEK antibodies for juvenile rheumatoid arthritis (JRA),Methods. Anti-DEK autoantibodies were measured by enzyme-linked immunosorbent assay (ELISA) using affinity-purified his6-DEK fusion protein. Sera from 639 subjects (417 patients with systemic autoimmune disease, 13 with sarcoidosis, 44 with pulmonary tuberculosis, 125 with uveitis, and 6 with scleritis, and 34 healthy control subjects) were screened. Reactivity was verified by immunoblotting and immunoprecipitation Studies using baculovirus-expressed human DEK.Results. Anti-DEK activity was found at the following frequencies: JRA 39.4% (n = 71), systemic lupus erythematosus (SLE) 25.1% (n = 216), sarcoidosis 46.2% (n = 13), rheumatoid arthritis 15.5% (n = 71), systemic sclerosis 36.0% (n = 22), polymyositis 6.2% (n = 16), and adult Still's disease 0% (n = 21), Autoantibodies also were detected in 9.1% of tuberculosis sera (n = 44), but were undetectable in sera from the 34 healthy controls. Western blot and immunoprecipitation assay results correlated well with the ELISA findings. in general, levels of anti-DEK autoantibodies were higher in SLE than in other patient subsets, including JRA.Conclusion. Anti-DEK autoantibodies are less specific for JRA than previously believed. They are produced in association with a variety of inflammatory conditions, many of which are associated with granuloma formation and/or predominant Th1 cytokine production. Anti-DEK antibodies may be a marker for a subset of autoimmunity associated with interferon-gamma production rather than a particular disease subset. |
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3465 |
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Dong, X. W.Wang, J. S.Kabir, F. N.Shaw, M.Reed, A. M.Stein, L.Andrade, Luiz Eduardo Coelho [UNIFESP]Trevisani, Virgínia Fernandes Moça [UNIFESP]Miller, M. L.Fujii, T.Akizuki, M.Pachman, L. M.Satoh, M.Reeves, W. H.Univ N CarolinaUniversidade Federal de São Paulo (UNIFESP)Northwestern UnivKeio Univ Hosp2016-01-24T12:30:57Z2016-01-24T12:30:57Z2000-01-01Arthritis and Rheumatism. Philadelphia: Lippincott Williams & Wilkins, v. 43, n. 1, p. 85-93, 2000.0004-3591http://repositorio.unifesp.br/handle/11600/26198http://dx.doi.org/10.1002/1529-0131(200001)43:1<8510.1002/1529-0131(200001)43:1<85WOS:000084782700011Objective. To evaluate the specificity of anti-DEK antibodies for juvenile rheumatoid arthritis (JRA),Methods. Anti-DEK autoantibodies were measured by enzyme-linked immunosorbent assay (ELISA) using affinity-purified his6-DEK fusion protein. Sera from 639 subjects (417 patients with systemic autoimmune disease, 13 with sarcoidosis, 44 with pulmonary tuberculosis, 125 with uveitis, and 6 with scleritis, and 34 healthy control subjects) were screened. Reactivity was verified by immunoblotting and immunoprecipitation Studies using baculovirus-expressed human DEK.Results. Anti-DEK activity was found at the following frequencies: JRA 39.4% (n = 71), systemic lupus erythematosus (SLE) 25.1% (n = 216), sarcoidosis 46.2% (n = 13), rheumatoid arthritis 15.5% (n = 71), systemic sclerosis 36.0% (n = 22), polymyositis 6.2% (n = 16), and adult Still's disease 0% (n = 21), Autoantibodies also were detected in 9.1% of tuberculosis sera (n = 44), but were undetectable in sera from the 34 healthy controls. Western blot and immunoprecipitation assay results correlated well with the ELISA findings. in general, levels of anti-DEK autoantibodies were higher in SLE than in other patient subsets, including JRA.Conclusion. Anti-DEK autoantibodies are less specific for JRA than previously believed. They are produced in association with a variety of inflammatory conditions, many of which are associated with granuloma formation and/or predominant Th1 cytokine production. Anti-DEK antibodies may be a marker for a subset of autoimmunity associated with interferon-gamma production rather than a particular disease subset.Univ N Carolina, Thurston Arthrit Res Ctr, Chapel Hill, NC USAUniv N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USAUniversidade Federal de São Paulo, São Paulo, BrazilNorthwestern Univ, Sch Med, Chicago, IL USAKeio Univ Hosp, Tokyo, JapanUniversidade Federal de São Paulo, EPM, São Paulo, BrazilWeb of Science85-93engLippincott Williams & WilkinsArthritis and RheumatismAutoantibodies to DEK oncoprotein in human inflammatory diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/261982022-07-08 10:28:55.956metadata only accessoai:repositorio.unifesp.br:11600/26198Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:17:58.938884Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Autoantibodies to DEK oncoprotein in human inflammatory disease |
title |
Autoantibodies to DEK oncoprotein in human inflammatory disease |
spellingShingle |
Autoantibodies to DEK oncoprotein in human inflammatory disease Dong, X. W. |
title_short |
Autoantibodies to DEK oncoprotein in human inflammatory disease |
title_full |
Autoantibodies to DEK oncoprotein in human inflammatory disease |
title_fullStr |
Autoantibodies to DEK oncoprotein in human inflammatory disease |
title_full_unstemmed |
Autoantibodies to DEK oncoprotein in human inflammatory disease |
title_sort |
Autoantibodies to DEK oncoprotein in human inflammatory disease |
author |
Dong, X. W. |
author_facet |
Dong, X. W. Wang, J. S. Kabir, F. N. Shaw, M. Reed, A. M. Stein, L. Andrade, Luiz Eduardo Coelho [UNIFESP] Trevisani, Virgínia Fernandes Moça [UNIFESP] Miller, M. L. Fujii, T. Akizuki, M. Pachman, L. M. Satoh, M. Reeves, W. H. |
author_role |
author |
author2 |
Wang, J. S. Kabir, F. N. Shaw, M. Reed, A. M. Stein, L. Andrade, Luiz Eduardo Coelho [UNIFESP] Trevisani, Virgínia Fernandes Moça [UNIFESP] Miller, M. L. Fujii, T. Akizuki, M. Pachman, L. M. Satoh, M. Reeves, W. H. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Univ N Carolina Universidade Federal de São Paulo (UNIFESP) Northwestern Univ Keio Univ Hosp |
dc.contributor.author.fl_str_mv |
Dong, X. W. Wang, J. S. Kabir, F. N. Shaw, M. Reed, A. M. Stein, L. Andrade, Luiz Eduardo Coelho [UNIFESP] Trevisani, Virgínia Fernandes Moça [UNIFESP] Miller, M. L. Fujii, T. Akizuki, M. Pachman, L. M. Satoh, M. Reeves, W. H. |
description |
Objective. To evaluate the specificity of anti-DEK antibodies for juvenile rheumatoid arthritis (JRA),Methods. Anti-DEK autoantibodies were measured by enzyme-linked immunosorbent assay (ELISA) using affinity-purified his6-DEK fusion protein. Sera from 639 subjects (417 patients with systemic autoimmune disease, 13 with sarcoidosis, 44 with pulmonary tuberculosis, 125 with uveitis, and 6 with scleritis, and 34 healthy control subjects) were screened. Reactivity was verified by immunoblotting and immunoprecipitation Studies using baculovirus-expressed human DEK.Results. Anti-DEK activity was found at the following frequencies: JRA 39.4% (n = 71), systemic lupus erythematosus (SLE) 25.1% (n = 216), sarcoidosis 46.2% (n = 13), rheumatoid arthritis 15.5% (n = 71), systemic sclerosis 36.0% (n = 22), polymyositis 6.2% (n = 16), and adult Still's disease 0% (n = 21), Autoantibodies also were detected in 9.1% of tuberculosis sera (n = 44), but were undetectable in sera from the 34 healthy controls. Western blot and immunoprecipitation assay results correlated well with the ELISA findings. in general, levels of anti-DEK autoantibodies were higher in SLE than in other patient subsets, including JRA.Conclusion. Anti-DEK autoantibodies are less specific for JRA than previously believed. They are produced in association with a variety of inflammatory conditions, many of which are associated with granuloma formation and/or predominant Th1 cytokine production. Anti-DEK antibodies may be a marker for a subset of autoimmunity associated with interferon-gamma production rather than a particular disease subset. |
publishDate |
2000 |
dc.date.issued.fl_str_mv |
2000-01-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:30:57Z |
dc.date.available.fl_str_mv |
2016-01-24T12:30:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Arthritis and Rheumatism. Philadelphia: Lippincott Williams & Wilkins, v. 43, n. 1, p. 85-93, 2000. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/26198 http://dx.doi.org/10.1002/1529-0131(200001)43:1<85 |
dc.identifier.issn.none.fl_str_mv |
0004-3591 |
dc.identifier.doi.none.fl_str_mv |
10.1002/1529-0131(200001)43:1<85 |
dc.identifier.wos.none.fl_str_mv |
WOS:000084782700011 |
identifier_str_mv |
Arthritis and Rheumatism. Philadelphia: Lippincott Williams & Wilkins, v. 43, n. 1, p. 85-93, 2000. 0004-3591 10.1002/1529-0131(200001)43:1<85 WOS:000084782700011 |
url |
http://repositorio.unifesp.br/handle/11600/26198 http://dx.doi.org/10.1002/1529-0131(200001)43:1<85 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Arthritis and Rheumatism |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
85-93 |
dc.publisher.none.fl_str_mv |
Lippincott Williams & Wilkins |
publisher.none.fl_str_mv |
Lippincott Williams & Wilkins |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1783460273553670144 |