Apoptotic Cells Contribute to Melanoma Progression and This Effect is Partially Mediated by the Platelet-Activating Factor Receptor
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/34373 http://dx.doi.org/10.1155/2012/610371 |
Resumo: | There is evidence that the platelet-activating factor receptor (PAFR) is involved in the clearance of apoptotic cells by macrophages, and that this is associated with anti-inflammatory phenotype. Our group has previously shown that coinjection of a large number of apoptotic cells can promote tumor growth from a subtumorigenic dose of melanoma cells. Here, we studied the involvement of the PAFR in the tumor growth promoting effect of apoptotic cells. A sub-tumorigenic dose of melanoma cells (Tm1) was coinjected with apoptotic Tm1 cells, subcutaneously in the flank of C57Bl/6 mice, and the volume was monitored for 30 days. Animals received the PAFR antagonists, WEB2170 or PCA4248 (5 mg/kg body weight) or vehicle, by peritumoral daily injection for 5 days. Results showed that PAFR antagonists significantly inhibited the tumor growth induced by the coinjection of a subtumorigenic dose of melanoma cells together with apoptotic cells. This was accompanied by inhibition of early neutrophil and macrophage infiltration. Addition of (platelet-activating factor) to this system has no significant effect. PAFR antagonists did not affect the promoting effect of carrageenan. We suggest that the recognition of apoptotic cells by phagocytes leads to activation of PAFR pathways, resulting in a microenvironment response favorable to melanoma growth. |
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Lacerda Bachi, Andre Luis [UNIFESP]Santos, Livia Caires dos [UNIFESP]Nonogaki, SuelyJancar, SoniaJasiulionis, Miriam Galvonas [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Adolfo Lutz InstUniversidade de São Paulo (USP)2016-01-24T14:17:37Z2016-01-24T14:17:37Z2012-01-01Mediators of Inflammation. New York: Hindawi Publishing Corporation, 6 p., 2012.0962-9351http://repositorio.unifesp.br/handle/11600/34373http://dx.doi.org/10.1155/2012/610371WOS000303710300001.pdf10.1155/2012/610371WOS:000303710300001There is evidence that the platelet-activating factor receptor (PAFR) is involved in the clearance of apoptotic cells by macrophages, and that this is associated with anti-inflammatory phenotype. Our group has previously shown that coinjection of a large number of apoptotic cells can promote tumor growth from a subtumorigenic dose of melanoma cells. Here, we studied the involvement of the PAFR in the tumor growth promoting effect of apoptotic cells. A sub-tumorigenic dose of melanoma cells (Tm1) was coinjected with apoptotic Tm1 cells, subcutaneously in the flank of C57Bl/6 mice, and the volume was monitored for 30 days. Animals received the PAFR antagonists, WEB2170 or PCA4248 (5 mg/kg body weight) or vehicle, by peritumoral daily injection for 5 days. Results showed that PAFR antagonists significantly inhibited the tumor growth induced by the coinjection of a subtumorigenic dose of melanoma cells together with apoptotic cells. This was accompanied by inhibition of early neutrophil and macrophage infiltration. Addition of (platelet-activating factor) to this system has no significant effect. PAFR antagonists did not affect the promoting effect of carrageenan. We suggest that the recognition of apoptotic cells by phagocytes leads to activation of PAFR pathways, resulting in a microenvironment response favorable to melanoma growth.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023900 São Paulo, BrazilAdolfo Lutz Inst, Div Pathol, BR-01246000 São Paulo, BrazilUniv São Paulo, Dept Immunol, BR-05508900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pharmacol, BR-04023032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pharmacol, BR-04023032 São Paulo, BrazilFAPESP: 06/61293-1Web of Science6engHindawi Publishing CorporationMediators of InflammationApoptotic Cells Contribute to Melanoma Progression and This Effect is Partially Mediated by the Platelet-Activating Factor Receptorinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000303710300001.pdfapplication/pdf1344686${dspace.ui.url}/bitstream/11600/34373/1/WOS000303710300001.pdfe4ae91ff1b30d7938f8813465bb31eabMD51open accessTEXTWOS000303710300001.pdf.txtWOS000303710300001.pdf.txtExtracted texttext/plain26118${dspace.ui.url}/bitstream/11600/34373/2/WOS000303710300001.pdf.txt5a211f70ce07300cd4cc964f51dff5dcMD52open access11600/343732022-02-18 10:14:11.364open accessoai:repositorio.unifesp.br:11600/34373Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:08:03.323497Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Apoptotic Cells Contribute to Melanoma Progression and This Effect is Partially Mediated by the Platelet-Activating Factor Receptor |
title |
Apoptotic Cells Contribute to Melanoma Progression and This Effect is Partially Mediated by the Platelet-Activating Factor Receptor |
spellingShingle |
Apoptotic Cells Contribute to Melanoma Progression and This Effect is Partially Mediated by the Platelet-Activating Factor Receptor Lacerda Bachi, Andre Luis [UNIFESP] |
title_short |
Apoptotic Cells Contribute to Melanoma Progression and This Effect is Partially Mediated by the Platelet-Activating Factor Receptor |
title_full |
Apoptotic Cells Contribute to Melanoma Progression and This Effect is Partially Mediated by the Platelet-Activating Factor Receptor |
title_fullStr |
Apoptotic Cells Contribute to Melanoma Progression and This Effect is Partially Mediated by the Platelet-Activating Factor Receptor |
title_full_unstemmed |
Apoptotic Cells Contribute to Melanoma Progression and This Effect is Partially Mediated by the Platelet-Activating Factor Receptor |
title_sort |
Apoptotic Cells Contribute to Melanoma Progression and This Effect is Partially Mediated by the Platelet-Activating Factor Receptor |
author |
Lacerda Bachi, Andre Luis [UNIFESP] |
author_facet |
Lacerda Bachi, Andre Luis [UNIFESP] Santos, Livia Caires dos [UNIFESP] Nonogaki, Suely Jancar, Sonia Jasiulionis, Miriam Galvonas [UNIFESP] |
author_role |
author |
author2 |
Santos, Livia Caires dos [UNIFESP] Nonogaki, Suely Jancar, Sonia Jasiulionis, Miriam Galvonas [UNIFESP] |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Adolfo Lutz Inst Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Lacerda Bachi, Andre Luis [UNIFESP] Santos, Livia Caires dos [UNIFESP] Nonogaki, Suely Jancar, Sonia Jasiulionis, Miriam Galvonas [UNIFESP] |
description |
There is evidence that the platelet-activating factor receptor (PAFR) is involved in the clearance of apoptotic cells by macrophages, and that this is associated with anti-inflammatory phenotype. Our group has previously shown that coinjection of a large number of apoptotic cells can promote tumor growth from a subtumorigenic dose of melanoma cells. Here, we studied the involvement of the PAFR in the tumor growth promoting effect of apoptotic cells. A sub-tumorigenic dose of melanoma cells (Tm1) was coinjected with apoptotic Tm1 cells, subcutaneously in the flank of C57Bl/6 mice, and the volume was monitored for 30 days. Animals received the PAFR antagonists, WEB2170 or PCA4248 (5 mg/kg body weight) or vehicle, by peritumoral daily injection for 5 days. Results showed that PAFR antagonists significantly inhibited the tumor growth induced by the coinjection of a subtumorigenic dose of melanoma cells together with apoptotic cells. This was accompanied by inhibition of early neutrophil and macrophage infiltration. Addition of (platelet-activating factor) to this system has no significant effect. PAFR antagonists did not affect the promoting effect of carrageenan. We suggest that the recognition of apoptotic cells by phagocytes leads to activation of PAFR pathways, resulting in a microenvironment response favorable to melanoma growth. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-01-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:17:37Z |
dc.date.available.fl_str_mv |
2016-01-24T14:17:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Mediators of Inflammation. New York: Hindawi Publishing Corporation, 6 p., 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/34373 http://dx.doi.org/10.1155/2012/610371 |
dc.identifier.issn.none.fl_str_mv |
0962-9351 |
dc.identifier.file.none.fl_str_mv |
WOS000303710300001.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1155/2012/610371 |
dc.identifier.wos.none.fl_str_mv |
WOS:000303710300001 |
identifier_str_mv |
Mediators of Inflammation. New York: Hindawi Publishing Corporation, 6 p., 2012. 0962-9351 WOS000303710300001.pdf 10.1155/2012/610371 WOS:000303710300001 |
url |
http://repositorio.unifesp.br/handle/11600/34373 http://dx.doi.org/10.1155/2012/610371 |
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eng |
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Mediators of Inflammation |
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6 |
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Hindawi Publishing Corporation |
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Hindawi Publishing Corporation |
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