Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis

Detalhes bibliográficos
Autor(a) principal: Albuquerque, C. F. [UNIFESP]
Data de Publicação: 2005
Outros Autores: Silva, Silvia HM da [UNIFESP], Camargo, Zoilo P. [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/28206
http://dx.doi.org/10.1128/JCM.43.4.1944-1946.2005
Resumo: In an attempt to improve the specificity of an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paracoccidioidomycosis (PCM), sera from patients with PCM were tested using various approaches, such as sodium metaperiodate antigen (gp43) treatment, a serum absorption process with Candida albicans or Histoplasma capsulatum antigens, and dilution of serum in galactose, the main common epitope among pathogenic fungi. the maximum specificity found in this ELISA was 84%. All of these procedures proved inefficient for eliminating all cross-reacting antibodies and obtaining an ELISA specific for PCM diagnosis.
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spelling Albuquerque, C. F. [UNIFESP]Silva, Silvia HM da [UNIFESP]Camargo, Zoilo P. [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T12:37:44Z2016-01-24T12:37:44Z2005-04-01Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 4, p. 1944-1946, 2005.0095-1137http://repositorio.unifesp.br/handle/11600/28206http://dx.doi.org/10.1128/JCM.43.4.1944-1946.2005WOS000228404100070.pdf10.1128/JCM.43.4.1944-1946.2005WOS:000228404100070In an attempt to improve the specificity of an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paracoccidioidomycosis (PCM), sera from patients with PCM were tested using various approaches, such as sodium metaperiodate antigen (gp43) treatment, a serum absorption process with Candida albicans or Histoplasma capsulatum antigens, and dilution of serum in galactose, the main common epitope among pathogenic fungi. the maximum specificity found in this ELISA was 84%. All of these procedures proved inefficient for eliminating all cross-reacting antibodies and obtaining an ELISA specific for PCM diagnosis.Universidade Federal de São Paulo, EPM, Dept Microbiol Imunol & Parasitol, Disciplina BIol Celular, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, EPM, Dept Microbiol Imunol & Parasitol, Disciplina BIol Celular, BR-04023062 São Paulo, BrazilWeb of Science1944-1946engAmer Soc MicrobiologyJournal of Clinical MicrobiologyImprovement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000228404100070.pdfapplication/pdf58638${dspace.ui.url}/bitstream/11600/28206/1/WOS000228404100070.pdf7bb5124d1657975facfc0355909ebacdMD51open accessTEXTWOS000228404100070.pdf.txtWOS000228404100070.pdf.txtExtracted texttext/plain19397${dspace.ui.url}/bitstream/11600/28206/2/WOS000228404100070.pdf.txtb60043f0d85619493f7192707dfb3392MD52open access11600/282062023-01-12 22:12:02.949open accessoai:repositorio.unifesp.br:11600/28206Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:30:05.066317Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis
title Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis
spellingShingle Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis
Albuquerque, C. F. [UNIFESP]
title_short Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis
title_full Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis
title_fullStr Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis
title_full_unstemmed Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis
title_sort Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis
author Albuquerque, C. F. [UNIFESP]
author_facet Albuquerque, C. F. [UNIFESP]
Silva, Silvia HM da [UNIFESP]
Camargo, Zoilo P. [UNIFESP]
author_role author
author2 Silva, Silvia HM da [UNIFESP]
Camargo, Zoilo P. [UNIFESP]
author2_role author
author
dc.contributor.institution.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Albuquerque, C. F. [UNIFESP]
Silva, Silvia HM da [UNIFESP]
Camargo, Zoilo P. [UNIFESP]
description In an attempt to improve the specificity of an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paracoccidioidomycosis (PCM), sera from patients with PCM were tested using various approaches, such as sodium metaperiodate antigen (gp43) treatment, a serum absorption process with Candida albicans or Histoplasma capsulatum antigens, and dilution of serum in galactose, the main common epitope among pathogenic fungi. the maximum specificity found in this ELISA was 84%. All of these procedures proved inefficient for eliminating all cross-reacting antibodies and obtaining an ELISA specific for PCM diagnosis.
publishDate 2005
dc.date.issued.fl_str_mv 2005-04-01
dc.date.accessioned.fl_str_mv 2016-01-24T12:37:44Z
dc.date.available.fl_str_mv 2016-01-24T12:37:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 4, p. 1944-1946, 2005.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/28206
http://dx.doi.org/10.1128/JCM.43.4.1944-1946.2005
dc.identifier.issn.none.fl_str_mv 0095-1137
dc.identifier.file.none.fl_str_mv WOS000228404100070.pdf
dc.identifier.doi.none.fl_str_mv 10.1128/JCM.43.4.1944-1946.2005
dc.identifier.wos.none.fl_str_mv WOS:000228404100070
identifier_str_mv Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 4, p. 1944-1946, 2005.
0095-1137
WOS000228404100070.pdf
10.1128/JCM.43.4.1944-1946.2005
WOS:000228404100070
url http://repositorio.unifesp.br/handle/11600/28206
http://dx.doi.org/10.1128/JCM.43.4.1944-1946.2005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Journal of Clinical Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1944-1946
dc.publisher.none.fl_str_mv Amer Soc Microbiology
publisher.none.fl_str_mv Amer Soc Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
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