Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/27769 http://dx.doi.org/10.1093/humrep/deh246 |
Resumo: | BACKGROUND: Many cancer survivors face infertility as a consequence of the aggressive treatment they must undergo. Cryopreservation of ovarian tissue before chemotherapy or radiotherapy may allow for tissue transplantation after the treatment, and restoration of fertility. We tested the potential of an orthotopic autografting of cryopreserved germinative tissue in female rabbits with ovarian failure following radiotherapy. METHODS: Ten adult multiparous female rabbits were randomly allocated into two groups, five in group I (control) and five in group II (transplant). All rabbits underwent right oophorectomy with cryopreservation of the germinative tissue, followed by sterilization of the remaining left ovary by radiotherapy. Later, group II rabbits received in the irradiated left ovary an implant of the frozen germinative tissue from the right ovary, whose small pieces were freely spread intracortically in a procedure we named 'intracortical sowing of germinative tissue' (ISGT). RESULTS: All group II rabbits conceived following spontaneous mating within 6 months of the transplant, whereas none of the remaining rabbits in group I had conceived up to 11 months after transplant. CONCLUSIONS: This study suggests that fertility can be restored in rabbits by sowing cortical tissue in a previously irradiated ovary. the clinical feasibility of this technique remains to be determined. |
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Almodin, Carlos Gilberto [UNIFESP]Minguetti-Camara, V. C.Meister, H.Ferreira, JOHRFranco, R. L.Cavalcante, A. A.Radaelli, MRMBahls, A. S.Moron, Antonio Fernandes [UNIFESP]|Murta, Carlos Geraldo Viana [UNIFESP]Materbaby Reprod Humana & GenetUniversidade Estadual de Maringá (UEM)Universidade Federal de São Paulo (UNIFESP)Univ Fed Espirito Santo2016-01-24T12:37:11Z2016-01-24T12:37:11Z2004-06-01Human Reproduction. Oxford: Oxford Univ Press, v. 19, n. 6, p. 1287-1293, 2004.0268-1161http://repositorio.unifesp.br/handle/11600/27769http://dx.doi.org/10.1093/humrep/deh24610.1093/humrep/deh246WOS:000221748200009BACKGROUND: Many cancer survivors face infertility as a consequence of the aggressive treatment they must undergo. Cryopreservation of ovarian tissue before chemotherapy or radiotherapy may allow for tissue transplantation after the treatment, and restoration of fertility. We tested the potential of an orthotopic autografting of cryopreserved germinative tissue in female rabbits with ovarian failure following radiotherapy. METHODS: Ten adult multiparous female rabbits were randomly allocated into two groups, five in group I (control) and five in group II (transplant). All rabbits underwent right oophorectomy with cryopreservation of the germinative tissue, followed by sterilization of the remaining left ovary by radiotherapy. Later, group II rabbits received in the irradiated left ovary an implant of the frozen germinative tissue from the right ovary, whose small pieces were freely spread intracortically in a procedure we named 'intracortical sowing of germinative tissue' (ISGT). RESULTS: All group II rabbits conceived following spontaneous mating within 6 months of the transplant, whereas none of the remaining rabbits in group I had conceived up to 11 months after transplant. CONCLUSIONS: This study suggests that fertility can be restored in rabbits by sowing cortical tissue in a previously irradiated ovary. the clinical feasibility of this technique remains to be determined.Materbaby Reprod Humana & Genet, BR-87013230 Maringa, Parana, BrazilUniv Estadual Maringa, Hosp Univ, Div Anal Clin, BR-87200000 Maringa, Parana, BrazilUniv Estadual Maringa, Dept Med, BR-87200000 Maringa, Parana, BrazilUniv Estadual Maringa, Dept Ciencias Morfofisiol, BR-87200000 Maringa, Parana, BrazilUniversidade Federal de São Paulo, Dept Obstet & Tocoginecol, BR-04905000 São Paulo, BrazilUniv Fed Espirito Santo, Dept Med, BR-29102000 Vitoria, ES, BrazilUniversidade Federal de São Paulo, Dept Obstet & Tocoginecol, BR-04905000 São Paulo, BrazilWeb of Science1287-1293engOxford Univ PressHuman Reproductionhttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlinfo:eu-repo/semantics/openAccessautograftcryopreservationfertilityovaryRecovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/277692023-01-27 22:45:17.072metadata only accessoai:repositorio.unifesp.br:11600/27769Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:44:19.562660Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy |
title |
Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy |
spellingShingle |
Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy Almodin, Carlos Gilberto [UNIFESP] autograft cryopreservation fertility ovary |
title_short |
Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy |
title_full |
Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy |
title_fullStr |
Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy |
title_full_unstemmed |
Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy |
title_sort |
Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy |
author |
Almodin, Carlos Gilberto [UNIFESP] |
author_facet |
Almodin, Carlos Gilberto [UNIFESP] Minguetti-Camara, V. C. Meister, H. Ferreira, JOHR Franco, R. L. Cavalcante, A. A. Radaelli, MRM Bahls, A. S. Moron, Antonio Fernandes [UNIFESP] |Murta, Carlos Geraldo Viana [UNIFESP] |
author_role |
author |
author2 |
Minguetti-Camara, V. C. Meister, H. Ferreira, JOHR Franco, R. L. Cavalcante, A. A. Radaelli, MRM Bahls, A. S. Moron, Antonio Fernandes [UNIFESP] |Murta, Carlos Geraldo Viana [UNIFESP] |
author2_role |
author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Materbaby Reprod Humana & Genet Universidade Estadual de Maringá (UEM) Universidade Federal de São Paulo (UNIFESP) Univ Fed Espirito Santo |
dc.contributor.author.fl_str_mv |
Almodin, Carlos Gilberto [UNIFESP] Minguetti-Camara, V. C. Meister, H. Ferreira, JOHR Franco, R. L. Cavalcante, A. A. Radaelli, MRM Bahls, A. S. Moron, Antonio Fernandes [UNIFESP] |Murta, Carlos Geraldo Viana [UNIFESP] |
dc.subject.eng.fl_str_mv |
autograft cryopreservation fertility ovary |
topic |
autograft cryopreservation fertility ovary |
description |
BACKGROUND: Many cancer survivors face infertility as a consequence of the aggressive treatment they must undergo. Cryopreservation of ovarian tissue before chemotherapy or radiotherapy may allow for tissue transplantation after the treatment, and restoration of fertility. We tested the potential of an orthotopic autografting of cryopreserved germinative tissue in female rabbits with ovarian failure following radiotherapy. METHODS: Ten adult multiparous female rabbits were randomly allocated into two groups, five in group I (control) and five in group II (transplant). All rabbits underwent right oophorectomy with cryopreservation of the germinative tissue, followed by sterilization of the remaining left ovary by radiotherapy. Later, group II rabbits received in the irradiated left ovary an implant of the frozen germinative tissue from the right ovary, whose small pieces were freely spread intracortically in a procedure we named 'intracortical sowing of germinative tissue' (ISGT). RESULTS: All group II rabbits conceived following spontaneous mating within 6 months of the transplant, whereas none of the remaining rabbits in group I had conceived up to 11 months after transplant. CONCLUSIONS: This study suggests that fertility can be restored in rabbits by sowing cortical tissue in a previously irradiated ovary. the clinical feasibility of this technique remains to be determined. |
publishDate |
2004 |
dc.date.issued.fl_str_mv |
2004-06-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:37:11Z |
dc.date.available.fl_str_mv |
2016-01-24T12:37:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Human Reproduction. Oxford: Oxford Univ Press, v. 19, n. 6, p. 1287-1293, 2004. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/27769 http://dx.doi.org/10.1093/humrep/deh246 |
dc.identifier.issn.none.fl_str_mv |
0268-1161 |
dc.identifier.doi.none.fl_str_mv |
10.1093/humrep/deh246 |
dc.identifier.wos.none.fl_str_mv |
WOS:000221748200009 |
identifier_str_mv |
Human Reproduction. Oxford: Oxford Univ Press, v. 19, n. 6, p. 1287-1293, 2004. 0268-1161 10.1093/humrep/deh246 WOS:000221748200009 |
url |
http://repositorio.unifesp.br/handle/11600/27769 http://dx.doi.org/10.1093/humrep/deh246 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Human Reproduction |
dc.rights.driver.fl_str_mv |
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1287-1293 |
dc.publisher.none.fl_str_mv |
Oxford Univ Press |
publisher.none.fl_str_mv |
Oxford Univ Press |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1783460241448370176 |