Heparanase expression in circulating lymphocytes of breast cancer patients depends on the presence of the primary tumor and/or systemic metastasis
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/29779 http://dx.doi.org/10.1593/neo.07241 |
Resumo: | Heparanase is an endo-beta-glucuronidase that is capable of degrading heparan sulfate chains of proteoglycans, generating a variety of bioactive molecules such as growth factors and chemotactic and angiogenic agents. the expression of heparanase was investigated in the peripheral blood mononuclear cell fraction ( PBMC) of 30 patients with breast cancer and 20 healthy control women by reverse transcription-polymerase chain reaction ( RT-PCR) and immunocytochemistry. PBMC samples from all breast cancer patients at study entry showed the expression of heparanase, whereas no expression was observed for healthy women. Immunocytochemistry analysis demonstrated that heparanase was expressed in lymphocytes of breast cancer PBMC. Throughout follow-up, heparanase expression by RTPCR decreased significantly after surgery in patients treated with neoadjuvant chemotherapy ( P = .002) and after tamoxifen treatment ( P = .040), whereas it increased significantly with the advent of systemic metastasis ( P = .027). in vitro, either serum from breast cancer patients or a medium originated from co-culture experiments of MCF-7 cells and lymphocytes from healthy women stimulated heparanase expression in normal lymphocytes. the results suggest that there is a tumor-inducing effect on heparanase expression by lymphocytes present in the PBMCs of breast cancer patients, which depends, in turn, on the interaction between a tumor and normal lymphocytes. |
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Theodoro, Therese RachellMatos, Leandro Luongo de [UNIFESP]Sant'Anna, Aleksandra Vanessa LambiasiFonseca, Fernando Luiz Affonso [UNIFESP]Semedo, Patricia [UNIFESP]Martins, Lourdes ConceicaoNader, Helena Bonciani [UNIFESP]Del Giglio, AuroPinhal, Maria Aparecida da Silva [UNIFESP]Fac Med ABCUniversidade Federal de São Paulo (UNIFESP)2016-01-24T13:48:45Z2016-01-24T13:48:45Z2007-06-01Neoplasia. Ann Arbor: Neoplasia Press, v. 9, n. 6, p. 504-510, 2007.1522-8002http://repositorio.unifesp.br/handle/11600/29779http://dx.doi.org/10.1593/neo.0724110.1593/neo.07241WOS:000247366800007Heparanase is an endo-beta-glucuronidase that is capable of degrading heparan sulfate chains of proteoglycans, generating a variety of bioactive molecules such as growth factors and chemotactic and angiogenic agents. the expression of heparanase was investigated in the peripheral blood mononuclear cell fraction ( PBMC) of 30 patients with breast cancer and 20 healthy control women by reverse transcription-polymerase chain reaction ( RT-PCR) and immunocytochemistry. PBMC samples from all breast cancer patients at study entry showed the expression of heparanase, whereas no expression was observed for healthy women. Immunocytochemistry analysis demonstrated that heparanase was expressed in lymphocytes of breast cancer PBMC. Throughout follow-up, heparanase expression by RTPCR decreased significantly after surgery in patients treated with neoadjuvant chemotherapy ( P = .002) and after tamoxifen treatment ( P = .040), whereas it increased significantly with the advent of systemic metastasis ( P = .027). in vitro, either serum from breast cancer patients or a medium originated from co-culture experiments of MCF-7 cells and lymphocytes from healthy women stimulated heparanase expression in normal lymphocytes. the results suggest that there is a tumor-inducing effect on heparanase expression by lymphocytes present in the PBMCs of breast cancer patients, which depends, in turn, on the interaction between a tumor and normal lymphocytes.Fac Med ABC, Dept Bioquim, São Paulo, BrazilFac Med ABC, Dept Oncol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, BrazilFac Med ABC, Dept Saude Comunitaria, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, BrazilWeb of Science504-510engNeoplasia PressNeoplasiaheparanasebreast cancerlymphocytestumor markersmetastasisHeparanase expression in circulating lymphocytes of breast cancer patients depends on the presence of the primary tumor and/or systemic metastasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/297792023-01-12 22:12:03.627metadata only accessoai:repositorio.unifesp.br:11600/29779Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:30:14.957361Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Heparanase expression in circulating lymphocytes of breast cancer patients depends on the presence of the primary tumor and/or systemic metastasis |
title |
Heparanase expression in circulating lymphocytes of breast cancer patients depends on the presence of the primary tumor and/or systemic metastasis |
spellingShingle |
Heparanase expression in circulating lymphocytes of breast cancer patients depends on the presence of the primary tumor and/or systemic metastasis Theodoro, Therese Rachell heparanase breast cancer lymphocytes tumor markers metastasis |
title_short |
Heparanase expression in circulating lymphocytes of breast cancer patients depends on the presence of the primary tumor and/or systemic metastasis |
title_full |
Heparanase expression in circulating lymphocytes of breast cancer patients depends on the presence of the primary tumor and/or systemic metastasis |
title_fullStr |
Heparanase expression in circulating lymphocytes of breast cancer patients depends on the presence of the primary tumor and/or systemic metastasis |
title_full_unstemmed |
Heparanase expression in circulating lymphocytes of breast cancer patients depends on the presence of the primary tumor and/or systemic metastasis |
title_sort |
Heparanase expression in circulating lymphocytes of breast cancer patients depends on the presence of the primary tumor and/or systemic metastasis |
author |
Theodoro, Therese Rachell |
author_facet |
Theodoro, Therese Rachell Matos, Leandro Luongo de [UNIFESP] Sant'Anna, Aleksandra Vanessa Lambiasi Fonseca, Fernando Luiz Affonso [UNIFESP] Semedo, Patricia [UNIFESP] Martins, Lourdes Conceicao Nader, Helena Bonciani [UNIFESP] Del Giglio, Auro Pinhal, Maria Aparecida da Silva [UNIFESP] |
author_role |
author |
author2 |
Matos, Leandro Luongo de [UNIFESP] Sant'Anna, Aleksandra Vanessa Lambiasi Fonseca, Fernando Luiz Affonso [UNIFESP] Semedo, Patricia [UNIFESP] Martins, Lourdes Conceicao Nader, Helena Bonciani [UNIFESP] Del Giglio, Auro Pinhal, Maria Aparecida da Silva [UNIFESP] |
author2_role |
author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Fac Med ABC Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Theodoro, Therese Rachell Matos, Leandro Luongo de [UNIFESP] Sant'Anna, Aleksandra Vanessa Lambiasi Fonseca, Fernando Luiz Affonso [UNIFESP] Semedo, Patricia [UNIFESP] Martins, Lourdes Conceicao Nader, Helena Bonciani [UNIFESP] Del Giglio, Auro Pinhal, Maria Aparecida da Silva [UNIFESP] |
dc.subject.eng.fl_str_mv |
heparanase breast cancer lymphocytes tumor markers metastasis |
topic |
heparanase breast cancer lymphocytes tumor markers metastasis |
description |
Heparanase is an endo-beta-glucuronidase that is capable of degrading heparan sulfate chains of proteoglycans, generating a variety of bioactive molecules such as growth factors and chemotactic and angiogenic agents. the expression of heparanase was investigated in the peripheral blood mononuclear cell fraction ( PBMC) of 30 patients with breast cancer and 20 healthy control women by reverse transcription-polymerase chain reaction ( RT-PCR) and immunocytochemistry. PBMC samples from all breast cancer patients at study entry showed the expression of heparanase, whereas no expression was observed for healthy women. Immunocytochemistry analysis demonstrated that heparanase was expressed in lymphocytes of breast cancer PBMC. Throughout follow-up, heparanase expression by RTPCR decreased significantly after surgery in patients treated with neoadjuvant chemotherapy ( P = .002) and after tamoxifen treatment ( P = .040), whereas it increased significantly with the advent of systemic metastasis ( P = .027). in vitro, either serum from breast cancer patients or a medium originated from co-culture experiments of MCF-7 cells and lymphocytes from healthy women stimulated heparanase expression in normal lymphocytes. the results suggest that there is a tumor-inducing effect on heparanase expression by lymphocytes present in the PBMCs of breast cancer patients, which depends, in turn, on the interaction between a tumor and normal lymphocytes. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-06-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T13:48:45Z |
dc.date.available.fl_str_mv |
2016-01-24T13:48:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Neoplasia. Ann Arbor: Neoplasia Press, v. 9, n. 6, p. 504-510, 2007. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/29779 http://dx.doi.org/10.1593/neo.07241 |
dc.identifier.issn.none.fl_str_mv |
1522-8002 |
dc.identifier.doi.none.fl_str_mv |
10.1593/neo.07241 |
dc.identifier.wos.none.fl_str_mv |
WOS:000247366800007 |
identifier_str_mv |
Neoplasia. Ann Arbor: Neoplasia Press, v. 9, n. 6, p. 504-510, 2007. 1522-8002 10.1593/neo.07241 WOS:000247366800007 |
url |
http://repositorio.unifesp.br/handle/11600/29779 http://dx.doi.org/10.1593/neo.07241 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Neoplasia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
504-510 |
dc.publisher.none.fl_str_mv |
Neoplasia Press |
publisher.none.fl_str_mv |
Neoplasia Press |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1783460299753390080 |