The renin-angiotensin system is upregulated in the cortex and hippocampus of patients with temporal lobe epilepsy related to mesial temporal sclerosis
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/30809 http://dx.doi.org/10.1111/j.1528-1167.2008.01581.x |
Resumo: | Purpose: As reported by several authors, angiotensin II (AngII) is a proinflammatory molecule that stimulates the release of inflammatory cytokines and activates nuclear factor kappa B (NF kappa B), being also associated with the increase of cellular oxidative stress. Its production depends on the activity of the angiotensin converting enzyme (ACE) that hydrolyzes the inactive precursor angiotensin I (AngI) into AngII. It has been suggested that AngII underlies the physiopathological mechanisms of several brain disorders such as stroke, bipolar disorder, schizophrenia, and disease. the aim of the present work was to localize and quantify AngII AT1 and AT2 receptors in the cortex and hippocampus of patients with temporal lobe epilepsy related to mesial temporal sclerosis (MTS) submitted to corticoamygdalohippocampectomy for seizure control.Method: Immunohistochemistry, Western blot, and real-time PCR techniques were employed to analyze the expression of these receptors.Results: the results showed an upregulation of AngII AT1 receptor as well as its messenger ribonucleic acid (mRNA) expression in the cortex and hippocampus of patients with MTS. in addition, an increased immunoexpression of AngII AT2 receptors was found only in the hippocampus of these patients with no changes in its mRNA levels.Discussion: These data show, for the first time, changes in components of renin-angiotensin system (RAS) that could be implicated in the physiopathology of MTS. |
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Arganaraz, Gustavo Adolfo [UNIFESP]Konno, Ana Carla [UNIFESP]Perosa, Sandra Regina [UNIFESP]Santiago, Joselita Ferreira Carvalho [UNIFESP]Boim, Mirian A. [UNIFESP]Vidotti, Daniela Berguio [UNIFESP]Varella, Pedro Paulo Vasconcellos [UNIFESP]Costa, Luciana Gilbert [UNIFESP]Canzian, MauroPorcionatto, Marimelia Aparecida [UNIFESP]Yacubian, Elza Márcia Targas [UNIFESP]Sakamoto, Americo Ceiki [UNIFESP]Carrete, Henrique [UNIFESP]Centeno, Ricardo Silva [UNIFESP]Amado, Debora [UNIFESP]Cavalheiro, Esper Abrao [UNIFESP]Silva, Jose AntonioNaffah-Mazzacoratti, Maria da Graca [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Nove Julho Univ2016-01-24T13:51:34Z2016-01-24T13:51:34Z2008-08-01Epilepsia. Oxford: Blackwell Publishing, v. 49, n. 8, p. 1348-1357, 2008.0013-9580http://repositorio.unifesp.br/handle/11600/30809http://dx.doi.org/10.1111/j.1528-1167.2008.01581.x10.1111/j.1528-1167.2008.01581.xWOS:000257988300008Purpose: As reported by several authors, angiotensin II (AngII) is a proinflammatory molecule that stimulates the release of inflammatory cytokines and activates nuclear factor kappa B (NF kappa B), being also associated with the increase of cellular oxidative stress. Its production depends on the activity of the angiotensin converting enzyme (ACE) that hydrolyzes the inactive precursor angiotensin I (AngI) into AngII. It has been suggested that AngII underlies the physiopathological mechanisms of several brain disorders such as stroke, bipolar disorder, schizophrenia, and disease. the aim of the present work was to localize and quantify AngII AT1 and AT2 receptors in the cortex and hippocampus of patients with temporal lobe epilepsy related to mesial temporal sclerosis (MTS) submitted to corticoamygdalohippocampectomy for seizure control.Method: Immunohistochemistry, Western blot, and real-time PCR techniques were employed to analyze the expression of these receptors.Results: the results showed an upregulation of AngII AT1 receptor as well as its messenger ribonucleic acid (mRNA) expression in the cortex and hippocampus of patients with MTS. in addition, an increased immunoexpression of AngII AT2 receptors was found only in the hippocampus of these patients with no changes in its mRNA levels.Discussion: These data show, for the first time, changes in components of renin-angiotensin system (RAS) that could be implicated in the physiopathology of MTS.Universidade Federal de São Paulo, Dept Neurol & Neurosurgery, Discipline Expt Neurol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Neurol & Neurosurgery, Discipline Nephrol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, São Paulo, BrazilUniv São Paulo, Sch Med, Dept Pathol, São Paulo, BrazilUniversidade Federal de São Paulo, Discipline Clin Neurol, Dept Biochem, São Paulo, BrazilUniversidade Federal de São Paulo, Discipline Clin Neurol, Dept Neurol & Neurosurg, São Paulo, BrazilNove Julho Univ, Dept Sci Rehabil, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Neurol & Neurosurgery, Discipline Expt Neurol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Neurol & Neurosurgery, Discipline Nephrol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, São Paulo, BrazilUniversidade Federal de São Paulo, Discipline Clin Neurol, Dept Biochem, São Paulo, BrazilUniversidade Federal de São Paulo, Discipline Clin Neurol, Dept Neurol & Neurosurg, São Paulo, BrazilWeb of Science1348-1357engBlackwell PublishingEpilepsiaangiotensin II AT1 and AT2 receptorstemporal lobe epilepsycortexhippocampusThe renin-angiotensin system is upregulated in the cortex and hippocampus of patients with temporal lobe epilepsy related to mesial temporal sclerosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/308092022-02-18 10:09:40.206metadata only accessoai:repositorio.unifesp.br:11600/30809Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:07:26.576722Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
The renin-angiotensin system is upregulated in the cortex and hippocampus of patients with temporal lobe epilepsy related to mesial temporal sclerosis |
title |
The renin-angiotensin system is upregulated in the cortex and hippocampus of patients with temporal lobe epilepsy related to mesial temporal sclerosis |
spellingShingle |
The renin-angiotensin system is upregulated in the cortex and hippocampus of patients with temporal lobe epilepsy related to mesial temporal sclerosis Arganaraz, Gustavo Adolfo [UNIFESP] angiotensin II AT1 and AT2 receptors temporal lobe epilepsy cortex hippocampus |
title_short |
The renin-angiotensin system is upregulated in the cortex and hippocampus of patients with temporal lobe epilepsy related to mesial temporal sclerosis |
title_full |
The renin-angiotensin system is upregulated in the cortex and hippocampus of patients with temporal lobe epilepsy related to mesial temporal sclerosis |
title_fullStr |
The renin-angiotensin system is upregulated in the cortex and hippocampus of patients with temporal lobe epilepsy related to mesial temporal sclerosis |
title_full_unstemmed |
The renin-angiotensin system is upregulated in the cortex and hippocampus of patients with temporal lobe epilepsy related to mesial temporal sclerosis |
title_sort |
The renin-angiotensin system is upregulated in the cortex and hippocampus of patients with temporal lobe epilepsy related to mesial temporal sclerosis |
author |
Arganaraz, Gustavo Adolfo [UNIFESP] |
author_facet |
Arganaraz, Gustavo Adolfo [UNIFESP] Konno, Ana Carla [UNIFESP] Perosa, Sandra Regina [UNIFESP] Santiago, Joselita Ferreira Carvalho [UNIFESP] Boim, Mirian A. [UNIFESP] Vidotti, Daniela Berguio [UNIFESP] Varella, Pedro Paulo Vasconcellos [UNIFESP] Costa, Luciana Gilbert [UNIFESP] Canzian, Mauro Porcionatto, Marimelia Aparecida [UNIFESP] Yacubian, Elza Márcia Targas [UNIFESP] Sakamoto, Americo Ceiki [UNIFESP] Carrete, Henrique [UNIFESP] Centeno, Ricardo Silva [UNIFESP] Amado, Debora [UNIFESP] Cavalheiro, Esper Abrao [UNIFESP] Silva, Jose Antonio Naffah-Mazzacoratti, Maria da Graca [UNIFESP] |
author_role |
author |
author2 |
Konno, Ana Carla [UNIFESP] Perosa, Sandra Regina [UNIFESP] Santiago, Joselita Ferreira Carvalho [UNIFESP] Boim, Mirian A. [UNIFESP] Vidotti, Daniela Berguio [UNIFESP] Varella, Pedro Paulo Vasconcellos [UNIFESP] Costa, Luciana Gilbert [UNIFESP] Canzian, Mauro Porcionatto, Marimelia Aparecida [UNIFESP] Yacubian, Elza Márcia Targas [UNIFESP] Sakamoto, Americo Ceiki [UNIFESP] Carrete, Henrique [UNIFESP] Centeno, Ricardo Silva [UNIFESP] Amado, Debora [UNIFESP] Cavalheiro, Esper Abrao [UNIFESP] Silva, Jose Antonio Naffah-Mazzacoratti, Maria da Graca [UNIFESP] |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) Nove Julho Univ |
dc.contributor.author.fl_str_mv |
Arganaraz, Gustavo Adolfo [UNIFESP] Konno, Ana Carla [UNIFESP] Perosa, Sandra Regina [UNIFESP] Santiago, Joselita Ferreira Carvalho [UNIFESP] Boim, Mirian A. [UNIFESP] Vidotti, Daniela Berguio [UNIFESP] Varella, Pedro Paulo Vasconcellos [UNIFESP] Costa, Luciana Gilbert [UNIFESP] Canzian, Mauro Porcionatto, Marimelia Aparecida [UNIFESP] Yacubian, Elza Márcia Targas [UNIFESP] Sakamoto, Americo Ceiki [UNIFESP] Carrete, Henrique [UNIFESP] Centeno, Ricardo Silva [UNIFESP] Amado, Debora [UNIFESP] Cavalheiro, Esper Abrao [UNIFESP] Silva, Jose Antonio Naffah-Mazzacoratti, Maria da Graca [UNIFESP] |
dc.subject.eng.fl_str_mv |
angiotensin II AT1 and AT2 receptors temporal lobe epilepsy cortex hippocampus |
topic |
angiotensin II AT1 and AT2 receptors temporal lobe epilepsy cortex hippocampus |
description |
Purpose: As reported by several authors, angiotensin II (AngII) is a proinflammatory molecule that stimulates the release of inflammatory cytokines and activates nuclear factor kappa B (NF kappa B), being also associated with the increase of cellular oxidative stress. Its production depends on the activity of the angiotensin converting enzyme (ACE) that hydrolyzes the inactive precursor angiotensin I (AngI) into AngII. It has been suggested that AngII underlies the physiopathological mechanisms of several brain disorders such as stroke, bipolar disorder, schizophrenia, and disease. the aim of the present work was to localize and quantify AngII AT1 and AT2 receptors in the cortex and hippocampus of patients with temporal lobe epilepsy related to mesial temporal sclerosis (MTS) submitted to corticoamygdalohippocampectomy for seizure control.Method: Immunohistochemistry, Western blot, and real-time PCR techniques were employed to analyze the expression of these receptors.Results: the results showed an upregulation of AngII AT1 receptor as well as its messenger ribonucleic acid (mRNA) expression in the cortex and hippocampus of patients with MTS. in addition, an increased immunoexpression of AngII AT2 receptors was found only in the hippocampus of these patients with no changes in its mRNA levels.Discussion: These data show, for the first time, changes in components of renin-angiotensin system (RAS) that could be implicated in the physiopathology of MTS. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-08-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T13:51:34Z |
dc.date.available.fl_str_mv |
2016-01-24T13:51:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Epilepsia. Oxford: Blackwell Publishing, v. 49, n. 8, p. 1348-1357, 2008. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/30809 http://dx.doi.org/10.1111/j.1528-1167.2008.01581.x |
dc.identifier.issn.none.fl_str_mv |
0013-9580 |
dc.identifier.doi.none.fl_str_mv |
10.1111/j.1528-1167.2008.01581.x |
dc.identifier.wos.none.fl_str_mv |
WOS:000257988300008 |
identifier_str_mv |
Epilepsia. Oxford: Blackwell Publishing, v. 49, n. 8, p. 1348-1357, 2008. 0013-9580 10.1111/j.1528-1167.2008.01581.x WOS:000257988300008 |
url |
http://repositorio.unifesp.br/handle/11600/30809 http://dx.doi.org/10.1111/j.1528-1167.2008.01581.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Epilepsia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1348-1357 |
dc.publisher.none.fl_str_mv |
Blackwell Publishing |
publisher.none.fl_str_mv |
Blackwell Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1783460252174254080 |