In vitro susceptibility of antifungal drugs against Sporothrix brasiliensis recovered from cats with sporotrichosis in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/57889 http://dx.doi.org/10.1093/mmy/myv039 |
Resumo: | Sporotrichosis is an important subcutaneous mycosis of humans and animals. Classically, the disease is acquired upon traumatic inoculation of Sporothrix propagules from contaminated soil and plant debris. In addition, the direct horizontal transmission of Sporothrix among animals and the resulting zoonotic infection in humans highlight an alternative and efficient rout of transmission through biting and scratching. Sporothrix brasiliensis is themost virulent species of the Sporothrix schenckii complex and is responsible for the long-lasting outbreak of feline sporotrichosis in Brazil. However, antifungal susceptibility data of animal-borne isolates is scarce. Therefore, this study evaluated the in vitro activity of amphotericin B, caspofungin, itraconazole, voriconazole, fluconazole, and ketoconazole against animal-borne isolates of S. brasiliensis. The susceptibility tests were performed through broth microdilution (M38-A2). The results show the relevant activity of itraconazole, amphotericin B, and ketoconazole against S. brasiliensis, with the following MIC ranges: 0.125-2, 0.125-4 and 0.0312-2 mu g/ml, respectively. Caspofungin was moderately effective, displaying higher variation in MIC values (0.25-64 mu g/ml). Voriconazole (2-64 mu g/ml) and fluconazole (62.5-500 mu g/ml) showed low activity against S. brasiliensis strains. This study contributed to the characterization of the in vitro antifungal susceptibility of strains of S. brasiliensis recovered from cats with sporotrichosis, which have recently been considered the main source of human infections. |
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Nogueira Brilhante, Raimunda Samia [UNIFESP]Rodrigues, Anderson Messias [UNIFESP]Costa Sidrim, Jose Julio [UNIFESP]Gadelha Rocha, Marcos Fabio [UNIFESP]Pereira, Sandro AntonioFerreira Gremiao, Isabella DibPacheco Schubach, Tania Mariade Camargo, Zoilo Pires [UNIFESP]2020-08-21T17:00:09Z2020-08-21T17:00:09Z2016Medical Mycology. Oxford, v. 54, n. 3, p. 275-279, 2016.1369-3786https://repositorio.unifesp.br/handle/11600/57889http://dx.doi.org/10.1093/mmy/myv03910.1093/mmy/myv039WOS:000372105200007Sporotrichosis is an important subcutaneous mycosis of humans and animals. Classically, the disease is acquired upon traumatic inoculation of Sporothrix propagules from contaminated soil and plant debris. In addition, the direct horizontal transmission of Sporothrix among animals and the resulting zoonotic infection in humans highlight an alternative and efficient rout of transmission through biting and scratching. Sporothrix brasiliensis is themost virulent species of the Sporothrix schenckii complex and is responsible for the long-lasting outbreak of feline sporotrichosis in Brazil. However, antifungal susceptibility data of animal-borne isolates is scarce. Therefore, this study evaluated the in vitro activity of amphotericin B, caspofungin, itraconazole, voriconazole, fluconazole, and ketoconazole against animal-borne isolates of S. brasiliensis. The susceptibility tests were performed through broth microdilution (M38-A2). The results show the relevant activity of itraconazole, amphotericin B, and ketoconazole against S. brasiliensis, with the following MIC ranges: 0.125-2, 0.125-4 and 0.0312-2 mu g/ml, respectively. Caspofungin was moderately effective, displaying higher variation in MIC values (0.25-64 mu g/ml). Voriconazole (2-64 mu g/ml) and fluconazole (62.5-500 mu g/ml) showed low activity against S. brasiliensis strains. This study contributed to the characterization of the in vitro antifungal susceptibility of strains of S. brasiliensis recovered from cats with sporotrichosis, which have recently been considered the main source of human infections.Sao Paulo Research Foundation (FAPESP)National Council for Scientific and Technological Development (CNPq 304779/20113)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Programa Estrategico de Apoio a Pesquisa em Saude VI - Fiocruz/CNPqFundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ)Univ Fed Ceara, Specialized Med Mycol Ctr, Postgrad Program Med Microbiol, Fortaleza, Ceara, BrazilUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Cellular Biol Div, Sao Paulo, BrazilFundacao Oswaldo Cruz, Evandro Chagas Natl Inst Infect Dis, Rio De Janeiro, BrazilUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Cellular Biol Div, Sao Paulo, BrazilFAPESP: 2011/07350-1FAPESP: 2009/54024-2CNPq: 304779/20113CAPES: AE1-0052-000650100/11Fiocruz/CNPq: 407771/2013-3FAPERJ: E-26/102.255/2013Web of Science275-279engOxford Univ PressMedical MycologySporothrix brasiliensiscatsantifungal susceptibilitysporotrichosisSporothrix schenckii complexIn vitro susceptibility of antifungal drugs against Sporothrix brasiliensis recovered from cats with sporotrichosis in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleOxford543info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/578892022-02-08 11:52:11.563metadata only accessoai:repositorio.unifesp.br:11600/57889Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:18:07.361143Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
In vitro susceptibility of antifungal drugs against Sporothrix brasiliensis recovered from cats with sporotrichosis in Brazil |
title |
In vitro susceptibility of antifungal drugs against Sporothrix brasiliensis recovered from cats with sporotrichosis in Brazil |
spellingShingle |
In vitro susceptibility of antifungal drugs against Sporothrix brasiliensis recovered from cats with sporotrichosis in Brazil Nogueira Brilhante, Raimunda Samia [UNIFESP] Sporothrix brasiliensis cats antifungal susceptibility sporotrichosis Sporothrix schenckii complex |
title_short |
In vitro susceptibility of antifungal drugs against Sporothrix brasiliensis recovered from cats with sporotrichosis in Brazil |
title_full |
In vitro susceptibility of antifungal drugs against Sporothrix brasiliensis recovered from cats with sporotrichosis in Brazil |
title_fullStr |
In vitro susceptibility of antifungal drugs against Sporothrix brasiliensis recovered from cats with sporotrichosis in Brazil |
title_full_unstemmed |
In vitro susceptibility of antifungal drugs against Sporothrix brasiliensis recovered from cats with sporotrichosis in Brazil |
title_sort |
In vitro susceptibility of antifungal drugs against Sporothrix brasiliensis recovered from cats with sporotrichosis in Brazil |
author |
Nogueira Brilhante, Raimunda Samia [UNIFESP] |
author_facet |
Nogueira Brilhante, Raimunda Samia [UNIFESP] Rodrigues, Anderson Messias [UNIFESP] Costa Sidrim, Jose Julio [UNIFESP] Gadelha Rocha, Marcos Fabio [UNIFESP] Pereira, Sandro Antonio Ferreira Gremiao, Isabella Dib Pacheco Schubach, Tania Maria de Camargo, Zoilo Pires [UNIFESP] |
author_role |
author |
author2 |
Rodrigues, Anderson Messias [UNIFESP] Costa Sidrim, Jose Julio [UNIFESP] Gadelha Rocha, Marcos Fabio [UNIFESP] Pereira, Sandro Antonio Ferreira Gremiao, Isabella Dib Pacheco Schubach, Tania Maria de Camargo, Zoilo Pires [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Nogueira Brilhante, Raimunda Samia [UNIFESP] Rodrigues, Anderson Messias [UNIFESP] Costa Sidrim, Jose Julio [UNIFESP] Gadelha Rocha, Marcos Fabio [UNIFESP] Pereira, Sandro Antonio Ferreira Gremiao, Isabella Dib Pacheco Schubach, Tania Maria de Camargo, Zoilo Pires [UNIFESP] |
dc.subject.eng.fl_str_mv |
Sporothrix brasiliensis cats antifungal susceptibility sporotrichosis Sporothrix schenckii complex |
topic |
Sporothrix brasiliensis cats antifungal susceptibility sporotrichosis Sporothrix schenckii complex |
description |
Sporotrichosis is an important subcutaneous mycosis of humans and animals. Classically, the disease is acquired upon traumatic inoculation of Sporothrix propagules from contaminated soil and plant debris. In addition, the direct horizontal transmission of Sporothrix among animals and the resulting zoonotic infection in humans highlight an alternative and efficient rout of transmission through biting and scratching. Sporothrix brasiliensis is themost virulent species of the Sporothrix schenckii complex and is responsible for the long-lasting outbreak of feline sporotrichosis in Brazil. However, antifungal susceptibility data of animal-borne isolates is scarce. Therefore, this study evaluated the in vitro activity of amphotericin B, caspofungin, itraconazole, voriconazole, fluconazole, and ketoconazole against animal-borne isolates of S. brasiliensis. The susceptibility tests were performed through broth microdilution (M38-A2). The results show the relevant activity of itraconazole, amphotericin B, and ketoconazole against S. brasiliensis, with the following MIC ranges: 0.125-2, 0.125-4 and 0.0312-2 mu g/ml, respectively. Caspofungin was moderately effective, displaying higher variation in MIC values (0.25-64 mu g/ml). Voriconazole (2-64 mu g/ml) and fluconazole (62.5-500 mu g/ml) showed low activity against S. brasiliensis strains. This study contributed to the characterization of the in vitro antifungal susceptibility of strains of S. brasiliensis recovered from cats with sporotrichosis, which have recently been considered the main source of human infections. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2020-08-21T17:00:09Z |
dc.date.available.fl_str_mv |
2020-08-21T17:00:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Medical Mycology. Oxford, v. 54, n. 3, p. 275-279, 2016. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/57889 http://dx.doi.org/10.1093/mmy/myv039 |
dc.identifier.issn.none.fl_str_mv |
1369-3786 |
dc.identifier.doi.none.fl_str_mv |
10.1093/mmy/myv039 |
dc.identifier.wos.none.fl_str_mv |
WOS:000372105200007 |
identifier_str_mv |
Medical Mycology. Oxford, v. 54, n. 3, p. 275-279, 2016. 1369-3786 10.1093/mmy/myv039 WOS:000372105200007 |
url |
https://repositorio.unifesp.br/handle/11600/57889 http://dx.doi.org/10.1093/mmy/myv039 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Medical Mycology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
275-279 |
dc.coverage.none.fl_str_mv |
Oxford |
dc.publisher.none.fl_str_mv |
Oxford Univ Press |
publisher.none.fl_str_mv |
Oxford Univ Press |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1783460274104172544 |