Long-Term Humoral and Cellular Immune Responses Elicited by a Heterologous Plasmodium vivax Apical Membrane Antigen 1 Protein Prime/Adenovirus Boost Immunization Protocol
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/33985 http://dx.doi.org/10.1128/IAI.05048-11 |
Resumo: | Apical membrane antigen 1 (AMA-1) is an invasion-related Plasmodium antigen that is expressed during both intracellular and extracellular asexual stages of the parasite's life cycle, making it an ideal target for induction of humoral and cellular immune responses that can protect against malaria. We show here that when it is administered as a recombinant protein (P) in Montanide ISA720 adjuvant, followed by a recombinant human type 5 adenovirus (Ad), intense and long-lasting Plasmodium vivax AMA-1-specific antibody responses (including both IgG1 and IgG2a), as well as proliferative memory T cell responses, can be detected in immunized mice. Memory T cells displayed both central (CD44(hi) CD62L(hi)) and effector (CD44(hi) CD62L(lo)) phenotypes, with the central memory phenotype prevailing (56% of AMA-1-specific proliferating cells). Considering the main traits of the memory immune responses induced against AMA-1, this particular sequence of immunogens (P followed by Ad), but no others (Ad/Ad, Ad/P, or P/P), displayed an optimal synergistic effect. These results give further support to the need for preclinical studies of P. vivax vaccine candidate AMA-1 administered in prime/boost protocols that include recombinant proteins and adenoviral vectors. |
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Maduro Bouillet, Leoneide EricaDias, Mariana OliveiraDorigo, Natalia AlvesMoura, Andrew DouglasRussell, BruceNosten, FrancoisRenia, LaurentBraga, Erika MartinsGazzinelli, Ricardo TostesRodrigues, Mauricio M.Soares, Irene S. [UNIFESP]Bruna-Romero, OscarUniversidade Federal de Minas Gerais (UFMG)Universidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Fiocruz MSAgcy Sci Technol & ResShoklo Malaria Res UnitChurchill HospMahidol Univ2016-01-24T14:17:07Z2016-01-24T14:17:07Z2011-09-01Infection and Immunity. Washington: Amer Soc Microbiology, v. 79, n. 9, p. 3642-3652, 2011.0019-9567http://repositorio.unifesp.br/handle/11600/33985http://dx.doi.org/10.1128/IAI.05048-11WOS000293891000016.pdf10.1128/IAI.05048-11WOS:000293891000016Apical membrane antigen 1 (AMA-1) is an invasion-related Plasmodium antigen that is expressed during both intracellular and extracellular asexual stages of the parasite's life cycle, making it an ideal target for induction of humoral and cellular immune responses that can protect against malaria. We show here that when it is administered as a recombinant protein (P) in Montanide ISA720 adjuvant, followed by a recombinant human type 5 adenovirus (Ad), intense and long-lasting Plasmodium vivax AMA-1-specific antibody responses (including both IgG1 and IgG2a), as well as proliferative memory T cell responses, can be detected in immunized mice. Memory T cells displayed both central (CD44(hi) CD62L(hi)) and effector (CD44(hi) CD62L(lo)) phenotypes, with the central memory phenotype prevailing (56% of AMA-1-specific proliferating cells). Considering the main traits of the memory immune responses induced against AMA-1, this particular sequence of immunogens (P followed by Ad), but no others (Ad/Ad, Ad/P, or P/P), displayed an optimal synergistic effect. These results give further support to the need for preclinical studies of P. vivax vaccine candidate AMA-1 administered in prime/boost protocols that include recombinant proteins and adenoviral vectors.Oswaldo Cruz Foundation (FIOCRUZ)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Wellcome Trust of Great BritainUniv Fed Minas Gerais, Inst Biol Sci, Dept Microbiol, Belo Horizonte, MG, BrazilUniv Fed Minas Gerais, Inst Biol Sci, Dept Parasitol, Belo Horizonte, MG, BrazilUniv Fed Minas Gerais, Inst Biol Sci, Dept Biochem & Immunol, Belo Horizonte, MG, BrazilUniv São Paulo, Fac Pharmaceut Sci, Dept Clin Anal & Toxicol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilFiocruz MS, Rene Rachou Res Ctr, Belo Horizonte, MG, BrazilAgcy Sci Technol & Res, Singapore Immunol Network, Singapore, SingaporeShoklo Malaria Res Unit, Mae Sot, Tak, ThailandChurchill Hosp, Ctr Clin Vaccinol & Trop Med, Oxford OX3 7LJ, EnglandMahidol Univ, Fac Trop Med, Bangkok, ThailandUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilWeb of Science3642-3652engAmer Soc MicrobiologyInfection and ImmunityLong-Term Humoral and Cellular Immune Responses Elicited by a Heterologous Plasmodium vivax Apical Membrane Antigen 1 Protein Prime/Adenovirus Boost Immunization Protocolinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000293891000016.pdfapplication/pdf2312670${dspace.ui.url}/bitstream/11600/33985/1/WOS000293891000016.pdfcab7e926d3b4e6d27933f577a717b4bcMD51open accessTEXTWOS000293891000016.pdf.txtWOS000293891000016.pdf.txtExtracted texttext/plain54790${dspace.ui.url}/bitstream/11600/33985/2/WOS000293891000016.pdf.txt03a8f44ef76ff83617a62979aae2155aMD52open access11600/339852023-01-12 22:12:03.191open accessoai:repositorio.unifesp.br:11600/33985Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:30:08.958987Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Long-Term Humoral and Cellular Immune Responses Elicited by a Heterologous Plasmodium vivax Apical Membrane Antigen 1 Protein Prime/Adenovirus Boost Immunization Protocol |
title |
Long-Term Humoral and Cellular Immune Responses Elicited by a Heterologous Plasmodium vivax Apical Membrane Antigen 1 Protein Prime/Adenovirus Boost Immunization Protocol |
spellingShingle |
Long-Term Humoral and Cellular Immune Responses Elicited by a Heterologous Plasmodium vivax Apical Membrane Antigen 1 Protein Prime/Adenovirus Boost Immunization Protocol Maduro Bouillet, Leoneide Erica |
title_short |
Long-Term Humoral and Cellular Immune Responses Elicited by a Heterologous Plasmodium vivax Apical Membrane Antigen 1 Protein Prime/Adenovirus Boost Immunization Protocol |
title_full |
Long-Term Humoral and Cellular Immune Responses Elicited by a Heterologous Plasmodium vivax Apical Membrane Antigen 1 Protein Prime/Adenovirus Boost Immunization Protocol |
title_fullStr |
Long-Term Humoral and Cellular Immune Responses Elicited by a Heterologous Plasmodium vivax Apical Membrane Antigen 1 Protein Prime/Adenovirus Boost Immunization Protocol |
title_full_unstemmed |
Long-Term Humoral and Cellular Immune Responses Elicited by a Heterologous Plasmodium vivax Apical Membrane Antigen 1 Protein Prime/Adenovirus Boost Immunization Protocol |
title_sort |
Long-Term Humoral and Cellular Immune Responses Elicited by a Heterologous Plasmodium vivax Apical Membrane Antigen 1 Protein Prime/Adenovirus Boost Immunization Protocol |
author |
Maduro Bouillet, Leoneide Erica |
author_facet |
Maduro Bouillet, Leoneide Erica Dias, Mariana Oliveira Dorigo, Natalia Alves Moura, Andrew Douglas Russell, Bruce Nosten, Francois Renia, Laurent Braga, Erika Martins Gazzinelli, Ricardo Tostes Rodrigues, Mauricio M. Soares, Irene S. [UNIFESP] Bruna-Romero, Oscar |
author_role |
author |
author2 |
Dias, Mariana Oliveira Dorigo, Natalia Alves Moura, Andrew Douglas Russell, Bruce Nosten, Francois Renia, Laurent Braga, Erika Martins Gazzinelli, Ricardo Tostes Rodrigues, Mauricio M. Soares, Irene S. [UNIFESP] Bruna-Romero, Oscar |
author2_role |
author author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG) Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) Fiocruz MS Agcy Sci Technol & Res Shoklo Malaria Res Unit Churchill Hosp Mahidol Univ |
dc.contributor.author.fl_str_mv |
Maduro Bouillet, Leoneide Erica Dias, Mariana Oliveira Dorigo, Natalia Alves Moura, Andrew Douglas Russell, Bruce Nosten, Francois Renia, Laurent Braga, Erika Martins Gazzinelli, Ricardo Tostes Rodrigues, Mauricio M. Soares, Irene S. [UNIFESP] Bruna-Romero, Oscar |
description |
Apical membrane antigen 1 (AMA-1) is an invasion-related Plasmodium antigen that is expressed during both intracellular and extracellular asexual stages of the parasite's life cycle, making it an ideal target for induction of humoral and cellular immune responses that can protect against malaria. We show here that when it is administered as a recombinant protein (P) in Montanide ISA720 adjuvant, followed by a recombinant human type 5 adenovirus (Ad), intense and long-lasting Plasmodium vivax AMA-1-specific antibody responses (including both IgG1 and IgG2a), as well as proliferative memory T cell responses, can be detected in immunized mice. Memory T cells displayed both central (CD44(hi) CD62L(hi)) and effector (CD44(hi) CD62L(lo)) phenotypes, with the central memory phenotype prevailing (56% of AMA-1-specific proliferating cells). Considering the main traits of the memory immune responses induced against AMA-1, this particular sequence of immunogens (P followed by Ad), but no others (Ad/Ad, Ad/P, or P/P), displayed an optimal synergistic effect. These results give further support to the need for preclinical studies of P. vivax vaccine candidate AMA-1 administered in prime/boost protocols that include recombinant proteins and adenoviral vectors. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-09-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:17:07Z |
dc.date.available.fl_str_mv |
2016-01-24T14:17:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Infection and Immunity. Washington: Amer Soc Microbiology, v. 79, n. 9, p. 3642-3652, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/33985 http://dx.doi.org/10.1128/IAI.05048-11 |
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0019-9567 |
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WOS000293891000016.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1128/IAI.05048-11 |
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WOS:000293891000016 |
identifier_str_mv |
Infection and Immunity. Washington: Amer Soc Microbiology, v. 79, n. 9, p. 3642-3652, 2011. 0019-9567 WOS000293891000016.pdf 10.1128/IAI.05048-11 WOS:000293891000016 |
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http://repositorio.unifesp.br/handle/11600/33985 http://dx.doi.org/10.1128/IAI.05048-11 |
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Amer Soc Microbiology |
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Amer Soc Microbiology |
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