Identification of New Genes Related to Virulence of Xanthomonas axonopodis Pv. Citri during Citrus Host Interactions
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/55488 http://dx.doi.org/10.4236/aim.2017.71003 |
Resumo: | A mutant library of the bacterium Xanthomonas citri subsp. citri strain 306 pathotype A (Xac), the causative agent of most aggressive Asiatic type A citrus canker, was screened regarding altered canker symptoms after inoculations into Citrus sinensis and Citrus limonia host leaves. Twenty-six mutants have shown phenotypic virulence changes and have respectively knocked out gene identified by sequencing. In vivo growth curves were obtained for nine mutants to quantify how the mutations could affect pathogen's adaptability to growth inside and attack host plant infected tissue. Among identified genes in mutated strains, we could find those that until now had not been reported as being involved in Xac adaptation and/or virulence, such as predicted to encode for xylose repressor-like protein (XAC Delta xylR), Fe-S oxidoredutase (XAC Delta aslB), helicase IV (XAC Delta helD), ubiquinol cytochrome c oxidoreductase iron-sulfur subunit (XAC Delta petA), chromosome partitioning protein (XAC Delta parB) and cell division protein FtsB (XAC Delta ftsB), in addition to genes predicted to encode for hypothetical proteins. The new genes found in this study as being relevant to adaptation and virulence, improve the understanding of Xac fitness during citrus plant attack and canker symptoms development. |
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Ferreira, Cristiano B.Moreira, Leandro M.Brigati, Joice B.Lima, Lonjore L.Ferro, Jesus A.Ferro, Maria I. T.de Oliveira, Julio C. F. [UNIFESP]2020-07-17T14:03:33Z2020-07-17T14:03:33Z2017Advances In Microbiology. Irvine, v. 7, n. 1, p. 22-46, 2017.2327-0810https://repositorio.unifesp.br/handle/11600/55488http://dx.doi.org/10.4236/aim.2017.71003WOS000407171800003.pdf10.4236/aim.2017.71003WOS:000407171800003A mutant library of the bacterium Xanthomonas citri subsp. citri strain 306 pathotype A (Xac), the causative agent of most aggressive Asiatic type A citrus canker, was screened regarding altered canker symptoms after inoculations into Citrus sinensis and Citrus limonia host leaves. Twenty-six mutants have shown phenotypic virulence changes and have respectively knocked out gene identified by sequencing. In vivo growth curves were obtained for nine mutants to quantify how the mutations could affect pathogen's adaptability to growth inside and attack host plant infected tissue. Among identified genes in mutated strains, we could find those that until now had not been reported as being involved in Xac adaptation and/or virulence, such as predicted to encode for xylose repressor-like protein (XAC Delta xylR), Fe-S oxidoredutase (XAC Delta aslB), helicase IV (XAC Delta helD), ubiquinol cytochrome c oxidoreductase iron-sulfur subunit (XAC Delta petA), chromosome partitioning protein (XAC Delta parB) and cell division protein FtsB (XAC Delta ftsB), in addition to genes predicted to encode for hypothetical proteins. The new genes found in this study as being relevant to adaptation and virulence, improve the understanding of Xac fitness during citrus plant attack and canker symptoms development.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESPCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior-CAPESCNPqFundo de Defesa da Citricultura (FUNDECITRUS)CAPESFUNDECITRUSFAPESPSao Paulo State Univ UNESP, Fac Agr Sci & Vet, Dept Technol, Jaboticabal, SP, BrazilCitriculture Res Ctr Sylvio Moreira IAC Campinas, Campinas, SP, BrazilUniv Fed Ouro Preto, Inst Exact & Biol Sci, Dept Biol Sci, Ouro Preto, MG, BrazilUniv Fed Ouro Preto, Res Ctr Biol Sci NUPEB, Inst Exact & Biol Sci, Dept Biol Sci, Ouro Preto, MG, BrazilSao Paulo State Univ UNESP, Fac Agr Sci & Vet, Plant Hlth Dept, Jaboticabal, SP, BrazilFed Univ Sao Paulo UNIFESP, Dept Biol Sci, Diadema, SP, BrazilFed Univ Sao Paulo UNIFESP, Dept Biol Sci, Diadema, SP, BrazilFAPESP: 04/02006-7Web of Science22-46engHans PublishersAdvances In MicrobiologyXanthomonas axonopodis Pv. CitriMutantsImpaired VirulenceIdentification of New Genes Related to Virulence of Xanthomonas axonopodis Pv. Citri during Citrus Host Interactionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleIrvine71info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000407171800003.pdfapplication/pdf6360354${dspace.ui.url}/bitstream/11600/55488/1/WOS000407171800003.pdfa027f46a3d6f8e7c479212477a34168fMD51open accessTEXTWOS000407171800003.pdf.txtWOS000407171800003.pdf.txtExtracted texttext/plain65398${dspace.ui.url}/bitstream/11600/55488/8/WOS000407171800003.pdf.txt05acbf6cfcac38e8a341d3e8e4efccfeMD58open accessTHUMBNAILWOS000407171800003.pdf.jpgWOS000407171800003.pdf.jpgIM Thumbnailimage/jpeg7324${dspace.ui.url}/bitstream/11600/55488/10/WOS000407171800003.pdf.jpg3dc91b5228f03a1392efacc20bd0fe76MD510open access11600/554882023-06-05 19:21:07.956open accessoai:repositorio.unifesp.br:11600/55488Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:21:07Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Identification of New Genes Related to Virulence of Xanthomonas axonopodis Pv. Citri during Citrus Host Interactions |
title |
Identification of New Genes Related to Virulence of Xanthomonas axonopodis Pv. Citri during Citrus Host Interactions |
spellingShingle |
Identification of New Genes Related to Virulence of Xanthomonas axonopodis Pv. Citri during Citrus Host Interactions Ferreira, Cristiano B. Xanthomonas axonopodis Pv. Citri Mutants Impaired Virulence |
title_short |
Identification of New Genes Related to Virulence of Xanthomonas axonopodis Pv. Citri during Citrus Host Interactions |
title_full |
Identification of New Genes Related to Virulence of Xanthomonas axonopodis Pv. Citri during Citrus Host Interactions |
title_fullStr |
Identification of New Genes Related to Virulence of Xanthomonas axonopodis Pv. Citri during Citrus Host Interactions |
title_full_unstemmed |
Identification of New Genes Related to Virulence of Xanthomonas axonopodis Pv. Citri during Citrus Host Interactions |
title_sort |
Identification of New Genes Related to Virulence of Xanthomonas axonopodis Pv. Citri during Citrus Host Interactions |
author |
Ferreira, Cristiano B. |
author_facet |
Ferreira, Cristiano B. Moreira, Leandro M. Brigati, Joice B. Lima, Lonjore L. Ferro, Jesus A. Ferro, Maria I. T. de Oliveira, Julio C. F. [UNIFESP] |
author_role |
author |
author2 |
Moreira, Leandro M. Brigati, Joice B. Lima, Lonjore L. Ferro, Jesus A. Ferro, Maria I. T. de Oliveira, Julio C. F. [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira, Cristiano B. Moreira, Leandro M. Brigati, Joice B. Lima, Lonjore L. Ferro, Jesus A. Ferro, Maria I. T. de Oliveira, Julio C. F. [UNIFESP] |
dc.subject.eng.fl_str_mv |
Xanthomonas axonopodis Pv. Citri Mutants Impaired Virulence |
topic |
Xanthomonas axonopodis Pv. Citri Mutants Impaired Virulence |
description |
A mutant library of the bacterium Xanthomonas citri subsp. citri strain 306 pathotype A (Xac), the causative agent of most aggressive Asiatic type A citrus canker, was screened regarding altered canker symptoms after inoculations into Citrus sinensis and Citrus limonia host leaves. Twenty-six mutants have shown phenotypic virulence changes and have respectively knocked out gene identified by sequencing. In vivo growth curves were obtained for nine mutants to quantify how the mutations could affect pathogen's adaptability to growth inside and attack host plant infected tissue. Among identified genes in mutated strains, we could find those that until now had not been reported as being involved in Xac adaptation and/or virulence, such as predicted to encode for xylose repressor-like protein (XAC Delta xylR), Fe-S oxidoredutase (XAC Delta aslB), helicase IV (XAC Delta helD), ubiquinol cytochrome c oxidoreductase iron-sulfur subunit (XAC Delta petA), chromosome partitioning protein (XAC Delta parB) and cell division protein FtsB (XAC Delta ftsB), in addition to genes predicted to encode for hypothetical proteins. The new genes found in this study as being relevant to adaptation and virulence, improve the understanding of Xac fitness during citrus plant attack and canker symptoms development. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-07-17T14:03:33Z |
dc.date.available.fl_str_mv |
2020-07-17T14:03:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Advances In Microbiology. Irvine, v. 7, n. 1, p. 22-46, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/55488 http://dx.doi.org/10.4236/aim.2017.71003 |
dc.identifier.issn.none.fl_str_mv |
2327-0810 |
dc.identifier.file.none.fl_str_mv |
WOS000407171800003.pdf |
dc.identifier.doi.none.fl_str_mv |
10.4236/aim.2017.71003 |
dc.identifier.wos.none.fl_str_mv |
WOS:000407171800003 |
identifier_str_mv |
Advances In Microbiology. Irvine, v. 7, n. 1, p. 22-46, 2017. 2327-0810 WOS000407171800003.pdf 10.4236/aim.2017.71003 WOS:000407171800003 |
url |
https://repositorio.unifesp.br/handle/11600/55488 http://dx.doi.org/10.4236/aim.2017.71003 |
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eng |
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Advances In Microbiology |
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Hans Publishers |
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Hans Publishers |
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