Does soaking time during disinfestation affect germination rates in Dendrobium?

Detalhes bibliográficos
Autor(a) principal: José Carlos Sorgato
Data de Publicação: 2020
Outros Autores: Schultz Soares, Jackeline, Scalon, Silvana de Paula Quintão, Targanski Sajovic Pereira, Suzana, de Freitas Brotto, Débora, Marlon Ribeiro, Luan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/42131
Resumo: Asymbiotic germination is considered an efficient and viable technique that can increase germination rates. The effect of type and concentration of disinfestants, and the exposure time to disinfestants may differ according to the plant species. Therefore, species-specific standardization of disinfestation agent and procedure is necessary to achieve optimal germination rates. The objective of this study was to determine a disinfestation methodology to increase in vitro germination rates and the early development of seedlings of Dendrobium nobile and Dendrobium phalaenopsis, using different times for seed disinfestation and different culture media. Seeds were disinfected by soaking in a 0.8% sodium hypochlorite solution for 5 or 15 min under aseptic conditions, after which seed suspensions were either washed with water or left unwashed. Next, they were seeded in culture flasks containing four different culture media (MS, ½MS, K, and VW). The flasks were then transferred to a growth room under controlled photoperiod and temperature, where they remained under an irradiance of 20 μmol m-2 s-1. Germination rates of the species were evaluated 45 days after placement in the culture flasks. A higher germination rate was observed when the seeds were triple washed, regardless of the culture medium or soaking time. Seed soaking disinfestation for 5 min is also recommended. MS and ½MS media were the most effective culture media in promoting in vitro germination of the species under study.  
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spelling Does soaking time during disinfestation affect germination rates in Dendrobium?O tempo de imersão durante a desinfestação influencia nas taxas de germinação de Dendrobium?Asymbiotic germinationSodium hypochloriteOrchidaceaeOrnamental speciesDendrobium nobileDendrobium phalaenopsisGerminação assimbióticaOrchidaceaeDendrobium nobileDendrobium phalaenopsisHipoclorito de sódioEspécies ornamentais Asymbiotic germination is considered an efficient and viable technique that can increase germination rates. The effect of type and concentration of disinfestants, and the exposure time to disinfestants may differ according to the plant species. Therefore, species-specific standardization of disinfestation agent and procedure is necessary to achieve optimal germination rates. The objective of this study was to determine a disinfestation methodology to increase in vitro germination rates and the early development of seedlings of Dendrobium nobile and Dendrobium phalaenopsis, using different times for seed disinfestation and different culture media. Seeds were disinfected by soaking in a 0.8% sodium hypochlorite solution for 5 or 15 min under aseptic conditions, after which seed suspensions were either washed with water or left unwashed. Next, they were seeded in culture flasks containing four different culture media (MS, ½MS, K, and VW). The flasks were then transferred to a growth room under controlled photoperiod and temperature, where they remained under an irradiance of 20 μmol m-2 s-1. Germination rates of the species were evaluated 45 days after placement in the culture flasks. A higher germination rate was observed when the seeds were triple washed, regardless of the culture medium or soaking time. Seed soaking disinfestation for 5 min is also recommended. MS and ½MS media were the most effective culture media in promoting in vitro germination of the species under study.  A germinação assimbiótica é considerada uma técnica eficiente e viável resultando em elevados percentuais de germinação. Apesar do sucesso dessa técnica, o tipo, a concentração e o tempo de exposição do agente desinfestante diferem, necessitando padronização para cada espécie. Assim, a padronização do agente desinfestante e do procedimento são necessários para o aumento das taxas germinativas. Objetivou-se com este trabalho determinar metodologia que aumente a taxa de germinação in vitro e favoreça o desenvolvimento inicial de plântulas de Dendrobium nobile e Dendrobium phalaenopsis, utilizando diferentes métodos de desinfestação de sementes e diferentes meios de cultura. Sementes, sob condições assépticas, foram desinfestadas em solução de hipoclorito de sódio a 0,8%, por cinco ou quinze minutos. Após esses períodos, as suspensões de sementes receberam ou não a tríplice lavagem com água. Em seguida, foram semeadas em frascos de cultivo que continham quatro diferentes meios de cultura (MS, MS½, K e VW). Posteriormente, foram transferidos para sala de crescimento com fotoperíodo e temperatura controlados, onde permaneceram sob irradiância de 20 µmol m-2 s-1. Quarenta e cinco dias após a semeadura foi avaliada a porcentagem de germinação das espécies estudadas. Os resultados neste trabalho indicam que, independentemente do meio de cultura ou do tempo de desinfestação, as sementes quando submetidas à tríplice lavagem apresentaram porcentagem de germinação superior a àquelas que não receberam este procedimento. Recomenda-se a desinfestação das sementes por 5 minutos. Os meios MS e MS½ foram os mais efetivos em promover a germinação in vitro dessas espécies.EDUFU2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4213110.14393/BJ-v36n1a2020-42131Bioscience Journal ; Vol. 36 No. 1 (2020): Jan./Feb.; 42-50Bioscience Journal ; v. 36 n. 1 (2020): Jan./Feb.; 42-501981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/42131/27508Copyright (c) 2020 José Carlos Sorgato, Jackeline Schultz Soares, Silvana de Paula Quintão Scalon, Suzana Targanski Sajovic Pereira, Débora de Freitas Brotto, Luan Marlon Ribeirohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessJosé Carlos SorgatoSchultz Soares, JackelineScalon, Silvana de Paula QuintãoTarganski Sajovic Pereira, Suzanade Freitas Brotto, DéboraMarlon Ribeiro, Luan2022-01-14T20:46:23Zoai:ojs.www.seer.ufu.br:article/42131Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-01-14T20:46:23Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Does soaking time during disinfestation affect germination rates in Dendrobium?
O tempo de imersão durante a desinfestação influencia nas taxas de germinação de Dendrobium?
title Does soaking time during disinfestation affect germination rates in Dendrobium?
spellingShingle Does soaking time during disinfestation affect germination rates in Dendrobium?
José Carlos Sorgato
Asymbiotic germination
Sodium hypochlorite
Orchidaceae
Ornamental species
Dendrobium nobile
Dendrobium phalaenopsis
Germinação assimbiótica
Orchidaceae
Dendrobium nobile
Dendrobium phalaenopsis
Hipoclorito de sódio
Espécies ornamentais
title_short Does soaking time during disinfestation affect germination rates in Dendrobium?
title_full Does soaking time during disinfestation affect germination rates in Dendrobium?
title_fullStr Does soaking time during disinfestation affect germination rates in Dendrobium?
title_full_unstemmed Does soaking time during disinfestation affect germination rates in Dendrobium?
title_sort Does soaking time during disinfestation affect germination rates in Dendrobium?
author José Carlos Sorgato
author_facet José Carlos Sorgato
Schultz Soares, Jackeline
Scalon, Silvana de Paula Quintão
Targanski Sajovic Pereira, Suzana
de Freitas Brotto, Débora
Marlon Ribeiro, Luan
author_role author
author2 Schultz Soares, Jackeline
Scalon, Silvana de Paula Quintão
Targanski Sajovic Pereira, Suzana
de Freitas Brotto, Débora
Marlon Ribeiro, Luan
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv José Carlos Sorgato
Schultz Soares, Jackeline
Scalon, Silvana de Paula Quintão
Targanski Sajovic Pereira, Suzana
de Freitas Brotto, Débora
Marlon Ribeiro, Luan
dc.subject.por.fl_str_mv Asymbiotic germination
Sodium hypochlorite
Orchidaceae
Ornamental species
Dendrobium nobile
Dendrobium phalaenopsis
Germinação assimbiótica
Orchidaceae
Dendrobium nobile
Dendrobium phalaenopsis
Hipoclorito de sódio
Espécies ornamentais
topic Asymbiotic germination
Sodium hypochlorite
Orchidaceae
Ornamental species
Dendrobium nobile
Dendrobium phalaenopsis
Germinação assimbiótica
Orchidaceae
Dendrobium nobile
Dendrobium phalaenopsis
Hipoclorito de sódio
Espécies ornamentais
description Asymbiotic germination is considered an efficient and viable technique that can increase germination rates. The effect of type and concentration of disinfestants, and the exposure time to disinfestants may differ according to the plant species. Therefore, species-specific standardization of disinfestation agent and procedure is necessary to achieve optimal germination rates. The objective of this study was to determine a disinfestation methodology to increase in vitro germination rates and the early development of seedlings of Dendrobium nobile and Dendrobium phalaenopsis, using different times for seed disinfestation and different culture media. Seeds were disinfected by soaking in a 0.8% sodium hypochlorite solution for 5 or 15 min under aseptic conditions, after which seed suspensions were either washed with water or left unwashed. Next, they were seeded in culture flasks containing four different culture media (MS, ½MS, K, and VW). The flasks were then transferred to a growth room under controlled photoperiod and temperature, where they remained under an irradiance of 20 μmol m-2 s-1. Germination rates of the species were evaluated 45 days after placement in the culture flasks. A higher germination rate was observed when the seeds were triple washed, regardless of the culture medium or soaking time. Seed soaking disinfestation for 5 min is also recommended. MS and ½MS media were the most effective culture media in promoting in vitro germination of the species under study.  
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/42131
10.14393/BJ-v36n1a2020-42131
url https://seer.ufu.br/index.php/biosciencejournal/article/view/42131
identifier_str_mv 10.14393/BJ-v36n1a2020-42131
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/42131/27508
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 36 No. 1 (2020): Jan./Feb.; 42-50
Bioscience Journal ; v. 36 n. 1 (2020): Jan./Feb.; 42-50
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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