Acute toxicity of Jatropha curcas oil on plant cell cycle

Detalhes bibliográficos
Autor(a) principal: Andrade-Vieira, Larissa Fonseca
Data de Publicação: 2019
Outros Autores: Botelho, Carolina Mastella, Laviola, Bruno Galvêas, Ferreira, Adésio, Fontes, Milene Miranda Praça
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/42562
Resumo: Today, a great interest in Jatropha-based products exists worldwide, mainly for the production of biofuel. However, the oil obtained from this plant is known to be toxic due to contained curcins and phorbol esters. Bioassays, including plant cytogenetic assays based on cell cycle observation, are useful for determining the toxicity of J. curcas oil. Hence, the aim of this study was to describe the mechanism of action of J. curcas oil by cell cycle analysis using Lactuca sativa as plant testing model. A decrease in root growth was observed, closely related to the reduction in mitotic index, along with an increase in condensed nuclei. J. curcas chemicals act both as aneugenic agents, leading to the formation of lagged, sticky chromosomes and c-metaphase cells, as well as clastogenic agents, inducing the formation of chromosome bridges and fragments. The cytotoxicity and genotoxicity of phorbol esters and other chemical components of J. curcas oil was determined and discussed.
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spelling Acute toxicity of Jatropha curcas oil on plant cell cycleToxicidade aguda do óleo de Jatropha curcas no ciclo celular de plantasAneugenic effects.Lactuca sativa.Mitotic indexPhorbol estersRoot growth.Biological SciencesToday, a great interest in Jatropha-based products exists worldwide, mainly for the production of biofuel. However, the oil obtained from this plant is known to be toxic due to contained curcins and phorbol esters. Bioassays, including plant cytogenetic assays based on cell cycle observation, are useful for determining the toxicity of J. curcas oil. Hence, the aim of this study was to describe the mechanism of action of J. curcas oil by cell cycle analysis using Lactuca sativa as plant testing model. A decrease in root growth was observed, closely related to the reduction in mitotic index, along with an increase in condensed nuclei. J. curcas chemicals act both as aneugenic agents, leading to the formation of lagged, sticky chromosomes and c-metaphase cells, as well as clastogenic agents, inducing the formation of chromosome bridges and fragments. The cytotoxicity and genotoxicity of phorbol esters and other chemical components of J. curcas oil was determined and discussed.Um grande interesse mundial existe em produtos à base de pinhão manso, principalmente para a produção de biocombustíveis. No entanto, o óleo obtido a partir desta planta é conhecidamente tóxico por conter curcina e ésteres de forbol. Bioensaios, incluindo ensaios citogenéticos em plantas-modelo com base na observação do ciclo celular, são úteis para determinar a toxicidade do óleo de J. curcas. Assim, o objetivo deste estudo foi descrever o mecanismo de ação do óleo de J. curcas por análise do ciclo celular usando Lactuca sativa como modelo de teste em plantas. Foi observada uma redução no crescimento das raízes, intimamente relacionada com a redução do índice mitótico e com um aumento de núcleos condensados. Os constituintes químicos de J. curcas atuam simultaneamente como agentes aneugênicos, levando à formação de cromossomos perdidos e pegajosos e células em c-metáfase, bem como agentes clastogênicos, induzindo a formação de pontes e fragmentos cromossômicos. A citotoxicidade e genotoxicidade do éster de forbol e outros componentes químicos do óleo de J. curcas foram determinados e discutidos.EDUFU2019-10-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4256210.14393/BJ-v35n5a2019-42562Bioscience Journal ; Vol. 35 No. 5 (2019): Sept./Oct.; 1544-1551Bioscience Journal ; v. 35 n. 5 (2019): Sept./Oct.; 1544-15511981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/42562/27080Brazil; ContemporaryCopyright (c) 2019 Larissa Fonseca Andrade-Vieira, Carolina Mastella Botelho, Bruno Galvêas Laviola, Adésio Ferreira, Milene Miranda Praça Fonteshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAndrade-Vieira, Larissa Fonseca Botelho, Carolina Mastella Laviola, Bruno Galvêas Ferreira, Adésio Fontes, Milene Miranda Praça2022-01-26T10:38:44Zoai:ojs.www.seer.ufu.br:article/42562Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-01-26T10:38:44Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Acute toxicity of Jatropha curcas oil on plant cell cycle
Toxicidade aguda do óleo de Jatropha curcas no ciclo celular de plantas
title Acute toxicity of Jatropha curcas oil on plant cell cycle
spellingShingle Acute toxicity of Jatropha curcas oil on plant cell cycle
Andrade-Vieira, Larissa Fonseca
Aneugenic effects.
Lactuca sativa.
Mitotic index
Phorbol esters
Root growth.
Biological Sciences
title_short Acute toxicity of Jatropha curcas oil on plant cell cycle
title_full Acute toxicity of Jatropha curcas oil on plant cell cycle
title_fullStr Acute toxicity of Jatropha curcas oil on plant cell cycle
title_full_unstemmed Acute toxicity of Jatropha curcas oil on plant cell cycle
title_sort Acute toxicity of Jatropha curcas oil on plant cell cycle
author Andrade-Vieira, Larissa Fonseca
author_facet Andrade-Vieira, Larissa Fonseca
Botelho, Carolina Mastella
Laviola, Bruno Galvêas
Ferreira, Adésio
Fontes, Milene Miranda Praça
author_role author
author2 Botelho, Carolina Mastella
Laviola, Bruno Galvêas
Ferreira, Adésio
Fontes, Milene Miranda Praça
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Andrade-Vieira, Larissa Fonseca
Botelho, Carolina Mastella
Laviola, Bruno Galvêas
Ferreira, Adésio
Fontes, Milene Miranda Praça
dc.subject.por.fl_str_mv Aneugenic effects.
Lactuca sativa.
Mitotic index
Phorbol esters
Root growth.
Biological Sciences
topic Aneugenic effects.
Lactuca sativa.
Mitotic index
Phorbol esters
Root growth.
Biological Sciences
description Today, a great interest in Jatropha-based products exists worldwide, mainly for the production of biofuel. However, the oil obtained from this plant is known to be toxic due to contained curcins and phorbol esters. Bioassays, including plant cytogenetic assays based on cell cycle observation, are useful for determining the toxicity of J. curcas oil. Hence, the aim of this study was to describe the mechanism of action of J. curcas oil by cell cycle analysis using Lactuca sativa as plant testing model. A decrease in root growth was observed, closely related to the reduction in mitotic index, along with an increase in condensed nuclei. J. curcas chemicals act both as aneugenic agents, leading to the formation of lagged, sticky chromosomes and c-metaphase cells, as well as clastogenic agents, inducing the formation of chromosome bridges and fragments. The cytotoxicity and genotoxicity of phorbol esters and other chemical components of J. curcas oil was determined and discussed.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/42562
10.14393/BJ-v35n5a2019-42562
url https://seer.ufu.br/index.php/biosciencejournal/article/view/42562
identifier_str_mv 10.14393/BJ-v35n5a2019-42562
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/42562/27080
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporary
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 35 No. 5 (2019): Sept./Oct.; 1544-1551
Bioscience Journal ; v. 35 n. 5 (2019): Sept./Oct.; 1544-1551
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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