Acute toxicity of Jatropha curcas oil on plant cell cycle
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/42562 |
Resumo: | Today, a great interest in Jatropha-based products exists worldwide, mainly for the production of biofuel. However, the oil obtained from this plant is known to be toxic due to contained curcins and phorbol esters. Bioassays, including plant cytogenetic assays based on cell cycle observation, are useful for determining the toxicity of J. curcas oil. Hence, the aim of this study was to describe the mechanism of action of J. curcas oil by cell cycle analysis using Lactuca sativa as plant testing model. A decrease in root growth was observed, closely related to the reduction in mitotic index, along with an increase in condensed nuclei. J. curcas chemicals act both as aneugenic agents, leading to the formation of lagged, sticky chromosomes and c-metaphase cells, as well as clastogenic agents, inducing the formation of chromosome bridges and fragments. The cytotoxicity and genotoxicity of phorbol esters and other chemical components of J. curcas oil was determined and discussed. |
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Acute toxicity of Jatropha curcas oil on plant cell cycleToxicidade aguda do óleo de Jatropha curcas no ciclo celular de plantasAneugenic effects.Lactuca sativa.Mitotic indexPhorbol estersRoot growth.Biological SciencesToday, a great interest in Jatropha-based products exists worldwide, mainly for the production of biofuel. However, the oil obtained from this plant is known to be toxic due to contained curcins and phorbol esters. Bioassays, including plant cytogenetic assays based on cell cycle observation, are useful for determining the toxicity of J. curcas oil. Hence, the aim of this study was to describe the mechanism of action of J. curcas oil by cell cycle analysis using Lactuca sativa as plant testing model. A decrease in root growth was observed, closely related to the reduction in mitotic index, along with an increase in condensed nuclei. J. curcas chemicals act both as aneugenic agents, leading to the formation of lagged, sticky chromosomes and c-metaphase cells, as well as clastogenic agents, inducing the formation of chromosome bridges and fragments. The cytotoxicity and genotoxicity of phorbol esters and other chemical components of J. curcas oil was determined and discussed.Um grande interesse mundial existe em produtos à base de pinhão manso, principalmente para a produção de biocombustíveis. No entanto, o óleo obtido a partir desta planta é conhecidamente tóxico por conter curcina e ésteres de forbol. Bioensaios, incluindo ensaios citogenéticos em plantas-modelo com base na observação do ciclo celular, são úteis para determinar a toxicidade do óleo de J. curcas. Assim, o objetivo deste estudo foi descrever o mecanismo de ação do óleo de J. curcas por análise do ciclo celular usando Lactuca sativa como modelo de teste em plantas. Foi observada uma redução no crescimento das raízes, intimamente relacionada com a redução do índice mitótico e com um aumento de núcleos condensados. Os constituintes químicos de J. curcas atuam simultaneamente como agentes aneugênicos, levando à formação de cromossomos perdidos e pegajosos e células em c-metáfase, bem como agentes clastogênicos, induzindo a formação de pontes e fragmentos cromossômicos. A citotoxicidade e genotoxicidade do éster de forbol e outros componentes químicos do óleo de J. curcas foram determinados e discutidos.EDUFU2019-10-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4256210.14393/BJ-v35n5a2019-42562Bioscience Journal ; Vol. 35 No. 5 (2019): Sept./Oct.; 1544-1551Bioscience Journal ; v. 35 n. 5 (2019): Sept./Oct.; 1544-15511981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/42562/27080Brazil; ContemporaryCopyright (c) 2019 Larissa Fonseca Andrade-Vieira, Carolina Mastella Botelho, Bruno Galvêas Laviola, Adésio Ferreira, Milene Miranda Praça Fonteshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAndrade-Vieira, Larissa Fonseca Botelho, Carolina Mastella Laviola, Bruno Galvêas Ferreira, Adésio Fontes, Milene Miranda Praça2022-01-26T10:38:44Zoai:ojs.www.seer.ufu.br:article/42562Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-01-26T10:38:44Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Acute toxicity of Jatropha curcas oil on plant cell cycle Toxicidade aguda do óleo de Jatropha curcas no ciclo celular de plantas |
title |
Acute toxicity of Jatropha curcas oil on plant cell cycle |
spellingShingle |
Acute toxicity of Jatropha curcas oil on plant cell cycle Andrade-Vieira, Larissa Fonseca Aneugenic effects. Lactuca sativa. Mitotic index Phorbol esters Root growth. Biological Sciences |
title_short |
Acute toxicity of Jatropha curcas oil on plant cell cycle |
title_full |
Acute toxicity of Jatropha curcas oil on plant cell cycle |
title_fullStr |
Acute toxicity of Jatropha curcas oil on plant cell cycle |
title_full_unstemmed |
Acute toxicity of Jatropha curcas oil on plant cell cycle |
title_sort |
Acute toxicity of Jatropha curcas oil on plant cell cycle |
author |
Andrade-Vieira, Larissa Fonseca |
author_facet |
Andrade-Vieira, Larissa Fonseca Botelho, Carolina Mastella Laviola, Bruno Galvêas Ferreira, Adésio Fontes, Milene Miranda Praça |
author_role |
author |
author2 |
Botelho, Carolina Mastella Laviola, Bruno Galvêas Ferreira, Adésio Fontes, Milene Miranda Praça |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Andrade-Vieira, Larissa Fonseca Botelho, Carolina Mastella Laviola, Bruno Galvêas Ferreira, Adésio Fontes, Milene Miranda Praça |
dc.subject.por.fl_str_mv |
Aneugenic effects. Lactuca sativa. Mitotic index Phorbol esters Root growth. Biological Sciences |
topic |
Aneugenic effects. Lactuca sativa. Mitotic index Phorbol esters Root growth. Biological Sciences |
description |
Today, a great interest in Jatropha-based products exists worldwide, mainly for the production of biofuel. However, the oil obtained from this plant is known to be toxic due to contained curcins and phorbol esters. Bioassays, including plant cytogenetic assays based on cell cycle observation, are useful for determining the toxicity of J. curcas oil. Hence, the aim of this study was to describe the mechanism of action of J. curcas oil by cell cycle analysis using Lactuca sativa as plant testing model. A decrease in root growth was observed, closely related to the reduction in mitotic index, along with an increase in condensed nuclei. J. curcas chemicals act both as aneugenic agents, leading to the formation of lagged, sticky chromosomes and c-metaphase cells, as well as clastogenic agents, inducing the formation of chromosome bridges and fragments. The cytotoxicity and genotoxicity of phorbol esters and other chemical components of J. curcas oil was determined and discussed. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/42562 10.14393/BJ-v35n5a2019-42562 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/42562 |
identifier_str_mv |
10.14393/BJ-v35n5a2019-42562 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/42562/27080 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 35 No. 5 (2019): Sept./Oct.; 1544-1551 Bioscience Journal ; v. 35 n. 5 (2019): Sept./Oct.; 1544-1551 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069080832770048 |