Enzymatic activity and gene expression related to drought stress tolerance in maize seeds and seedlings
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/53953 |
Resumo: | Drought stress is a major limiting factor for the development of maize, and the identification of the expression of genes related to this stress in seeds and seedlings can be an important tool to accelerate the selection process. The expression of genes related to tolerance to water deficit in seeds and in different tissues of maize seedlings were evaluated. Four tolerant genotypes (91-T, 32-T, 91x75-T, 32x75-T) and four non-tolerant genotypes (37-NT, 57-NT, 37x57-NT and 31x37-NT) were seeded in a substrate with 10% (stress) and 70% (control) water retention capacity. The expression of 4 enzymes were evaluated: catalase (CAT), peroxidase (PO), esterase (EST), and heat-resistant protein (HRP), as well as the relative expression of 6 genes: ZmLEA3, ZmPP2C, ZmCPK11, ZmDREB2A/2.1s, ZmDBP3 and ZmAN13 were evaluated in seed, shoots and roots of seedlings submitted or not to stress. There was variation in the expression of CAT, PO, SOD, EST and HRP enzymes among the evaluated genotypes and also in the different tissues evaluated. Higher expression of the CAT and PO was observed in the shoots. There was a greater expression of the EST in the genotypes non-tolerant to water deficit. HRP was expressed only in seeds. In the aerial part of maize seedlings, classified as tolerant, higher expression of genes ZmLEA3 and ZmCPK11 was observed. There was a higher expression of the ZmAN13 and ZmDREB2A/2.1S genes in roots developed under stress conditions and a higher expression of the ZmPP2C gene in seeds of line 91-T, which is classified as tolerant to drought stress. |
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Enzymatic activity and gene expression related to drought stress tolerance in maize seeds and seedlingsAbiotic stress ProteomicRT-qPCRZea mays.Agricultural SciencesDrought stress is a major limiting factor for the development of maize, and the identification of the expression of genes related to this stress in seeds and seedlings can be an important tool to accelerate the selection process. The expression of genes related to tolerance to water deficit in seeds and in different tissues of maize seedlings were evaluated. Four tolerant genotypes (91-T, 32-T, 91x75-T, 32x75-T) and four non-tolerant genotypes (37-NT, 57-NT, 37x57-NT and 31x37-NT) were seeded in a substrate with 10% (stress) and 70% (control) water retention capacity. The expression of 4 enzymes were evaluated: catalase (CAT), peroxidase (PO), esterase (EST), and heat-resistant protein (HRP), as well as the relative expression of 6 genes: ZmLEA3, ZmPP2C, ZmCPK11, ZmDREB2A/2.1s, ZmDBP3 and ZmAN13 were evaluated in seed, shoots and roots of seedlings submitted or not to stress. There was variation in the expression of CAT, PO, SOD, EST and HRP enzymes among the evaluated genotypes and also in the different tissues evaluated. Higher expression of the CAT and PO was observed in the shoots. There was a greater expression of the EST in the genotypes non-tolerant to water deficit. HRP was expressed only in seeds. In the aerial part of maize seedlings, classified as tolerant, higher expression of genes ZmLEA3 and ZmCPK11 was observed. There was a higher expression of the ZmAN13 and ZmDREB2A/2.1S genes in roots developed under stress conditions and a higher expression of the ZmPP2C gene in seeds of line 91-T, which is classified as tolerant to drought stress.O estresse hídrico é um dos fatores mais limitantes para o desenvolvimento da cultura do milho, e o conhecimento de genes relacionados a este estresse em sementes e em plântulas pode ser uma importante ferramenta para acelerar o processo de seleção. Nós avaliamos a expressão de genes relacionados à tolerância ao estresse hídrico em sementes e em diferentes tecidos de plântulas de milho. Quatro genótipos tolerantes (91-T, 32-T, 91x75-T e 32x75-T) e quatro não tolerantes (37-NT, 57-NT, 37x57-NT e 31x37-NT) foram semeadas em substrato contendo 10% (estresse) e 70% (controle) de capacidade de retenção de água no solo. Foi avaliada a expressão de 4 enzimas: catalase (CAT), peroxidase (PO), esterase (EST) e proteínas resistentes ao calor (HRP), e também a expressão de 6 genes: ZmLEA3, ZmPP2C, ZmCPK11, ZmDREB2A/2.1s, ZmDBP3 e ZmAN13 em sementes, parte aérea e raízes submetidas ou não ao estresse. A expressão da CAT, PO, SOD, EST e HRP variaram entre os genótipos avaliados e também em sementes, raiz e parte aérea de plântulas de milho. Maior expressão das enzimas CAT e PO foram observadas na parte aérea. Houve maior expressão da enzima EST nos genótipos não tolerantes ao estresse hídrico. As HRP se expressaram apenas em sementes. Maior expressão dos genes ZmLEA3 e ZmCPK11 foram observados na parte aérea de plântulas de milho, classificadas como tolerantes. Houve maior expressão dos genes ZmAN13 e ZmDREB2A/2.1S em raízes sob condição de estresse e uma maior expressão do gene ZmPP2C em sementes da linhagem 91-T, que é classificada como tolerante ao déficit hídrico.EDUFU2021-12-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/5395310.14393/BJ-v37n0a2021-53953Bioscience Journal ; Vol. 37 (2021): Continuous Publication; e37079Bioscience Journal ; v. 37 (2021): Continuous Publication; e370791981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/53953/32956Brazil; Contemporary Copyright (c) 2021 Milena Christy Santos, Édila Vilela de Resende Von Pinho, Heloisa Oliveira dos Santos, Danielle Rezende Vilela, Izabel Costa Silva Neta, Viviane Maria de Abreu, Renato Coelho de Castro Vasconcelloshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessChristy Santos, MilenaVilela de Resende Von Pinho, ÉdilaOliveira dos Santos, HeloisaRezende Vilela, DanielleCosta Silva Neta, IzabelMaria de Abreu, VivianeCoelho de Castro Vasconcellos, Renato2022-05-25T13:29:12Zoai:ojs.www.seer.ufu.br:article/53953Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-05-25T13:29:12Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Enzymatic activity and gene expression related to drought stress tolerance in maize seeds and seedlings |
title |
Enzymatic activity and gene expression related to drought stress tolerance in maize seeds and seedlings |
spellingShingle |
Enzymatic activity and gene expression related to drought stress tolerance in maize seeds and seedlings Christy Santos, Milena Abiotic stress Proteomic RT-qPCR Zea mays. Agricultural Sciences |
title_short |
Enzymatic activity and gene expression related to drought stress tolerance in maize seeds and seedlings |
title_full |
Enzymatic activity and gene expression related to drought stress tolerance in maize seeds and seedlings |
title_fullStr |
Enzymatic activity and gene expression related to drought stress tolerance in maize seeds and seedlings |
title_full_unstemmed |
Enzymatic activity and gene expression related to drought stress tolerance in maize seeds and seedlings |
title_sort |
Enzymatic activity and gene expression related to drought stress tolerance in maize seeds and seedlings |
author |
Christy Santos, Milena |
author_facet |
Christy Santos, Milena Vilela de Resende Von Pinho, Édila Oliveira dos Santos, Heloisa Rezende Vilela, Danielle Costa Silva Neta, Izabel Maria de Abreu, Viviane Coelho de Castro Vasconcellos, Renato |
author_role |
author |
author2 |
Vilela de Resende Von Pinho, Édila Oliveira dos Santos, Heloisa Rezende Vilela, Danielle Costa Silva Neta, Izabel Maria de Abreu, Viviane Coelho de Castro Vasconcellos, Renato |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Christy Santos, Milena Vilela de Resende Von Pinho, Édila Oliveira dos Santos, Heloisa Rezende Vilela, Danielle Costa Silva Neta, Izabel Maria de Abreu, Viviane Coelho de Castro Vasconcellos, Renato |
dc.subject.por.fl_str_mv |
Abiotic stress Proteomic RT-qPCR Zea mays. Agricultural Sciences |
topic |
Abiotic stress Proteomic RT-qPCR Zea mays. Agricultural Sciences |
description |
Drought stress is a major limiting factor for the development of maize, and the identification of the expression of genes related to this stress in seeds and seedlings can be an important tool to accelerate the selection process. The expression of genes related to tolerance to water deficit in seeds and in different tissues of maize seedlings were evaluated. Four tolerant genotypes (91-T, 32-T, 91x75-T, 32x75-T) and four non-tolerant genotypes (37-NT, 57-NT, 37x57-NT and 31x37-NT) were seeded in a substrate with 10% (stress) and 70% (control) water retention capacity. The expression of 4 enzymes were evaluated: catalase (CAT), peroxidase (PO), esterase (EST), and heat-resistant protein (HRP), as well as the relative expression of 6 genes: ZmLEA3, ZmPP2C, ZmCPK11, ZmDREB2A/2.1s, ZmDBP3 and ZmAN13 were evaluated in seed, shoots and roots of seedlings submitted or not to stress. There was variation in the expression of CAT, PO, SOD, EST and HRP enzymes among the evaluated genotypes and also in the different tissues evaluated. Higher expression of the CAT and PO was observed in the shoots. There was a greater expression of the EST in the genotypes non-tolerant to water deficit. HRP was expressed only in seeds. In the aerial part of maize seedlings, classified as tolerant, higher expression of genes ZmLEA3 and ZmCPK11 was observed. There was a higher expression of the ZmAN13 and ZmDREB2A/2.1S genes in roots developed under stress conditions and a higher expression of the ZmPP2C gene in seeds of line 91-T, which is classified as tolerant to drought stress. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/53953 10.14393/BJ-v37n0a2021-53953 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/53953 |
identifier_str_mv |
10.14393/BJ-v37n0a2021-53953 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/53953/32956 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 37 (2021): Continuous Publication; e37079 Bioscience Journal ; v. 37 (2021): Continuous Publication; e37079 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069082523074560 |