Desenvolvimento de nanopart?culas lip?dicas contendo paclitaxel

Detalhes bibliográficos
Autor(a) principal: Marcial, Sara Pacelli de Sousa
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFVJM
Texto Completo: http://acervo.ufvjm.edu.br/jspui/handle/1/1128
Resumo: ?rea de concentra??o: Ci?ncias farmac?uticas.
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spelling Marcial, Sara Pacelli de SousaLeite, Elaine AmaralCarneiro, GuilhermeVerly, Rodrigo MoreiraCarvalho Jr, ?lvaro Dutra deCarneiro, GuilhermeUniversidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)Leite, Elaine Amaral2017-01-17T18:55:36Z2017-01-17T18:55:36Z20162016MARCIAL, Sara Pacelli de Sousa. Desenvolvimento de nanopart?culas lip?dicas contendo paclitaxel. 2016. 98 p. Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Ci?ncias Farmac?uticas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2016.http://acervo.ufvjm.edu.br/jspui/handle/1/1128?rea de concentra??o: Ci?ncias farmac?uticas.Data de aprova??o ausente.Disponibiliza??o do conte?do parcial, conforme Termo de Autoriza??o no trabalho.O paclitaxel (PTX) ? um agente quimioter?pico que tem uma importante fun??o no tratamento de v?rios tipos de c?ncer, especialmente o c?ncer de mama. No entanto, a baixa solubilidade do PTX em meio aquoso (coeficiente de parti??o log = 3,96) representa uma limita??o para a administra??o intravenosa. A formula??o convencional do PTX cont?m uma alta concentra??o de Cremofor-EL? (derivado polietoxilado do ?leo de r?cino), o qual induz significante toxicidade, restringindo sua utiliza??o cl?nica. A encapsula??o do PTX em sistema de libera??o de f?rmacos pode melhorar a absor??o e aumentar a sua efic?cia terap?utica. Neste estudo, tr?s diferentes nanossistemas lip?dicos contendo PTX, nanopart?culas lip?dicas s?lidas (NLS), nanoemuls?o (NE) e carreadores lip?dicos nanoestruturados (CLN) foram preparados e as propriedades f?sico-qu?micas e a atividade citotoxicidade in vitro foram avaliadas. Em rela??o ao di?metro m?dio, o CLN branco mostrou valor de di?metro aproximadamente 2 e 1,7 vezes menor que os obtidos para NLS e NE, respectivamente. A presen?a de PTX levou a um aumento significativo no di?metro das part?culas em todos os sistemas avaliados, exceto no NE. Al?m disso, o aumento da concentra??o do f?rmaco (0,01% para 0,025%) produziu um aumento do di?metro para a prepara??o de CLN. Todas as formula??es com PTX mostraram ?ndice de polidispers?o superior a 0,3, exceto para NE-PTX na concentra??o do f?rmaco igual a 0,01% (p/v). Valores negativos de potencial zeta foram observados para todas as formula??es avaliadas. CLN-PTX foi o sistema mais est?vel ap?s armazenado por 30 dias a 4 ?C. O estudo de citotoxicidade nas linhagens celulares de c?ncer de mama (MDA-MB-231 e MCF-7) demonstrou atividade citot?xica mais pronunciada para CLN-PTX do que para o PTX livre em ambos as linhagens celulares do tumor. Baseado nesses resultados, CLN-PTX parece ser uma ferramenta potencial para o tratamento do c?ncer de mama.Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2016-12-20T19:20:11Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) sara_pacelli_sousa_marcial_parcial.pdf: 166236 bytes, checksum: c01c4f087337cd82c64b06b90a1cc4dc (MD5)Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-01-17T18:55:36Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) sara_pacelli_sousa_marcial_parcial.pdf: 166236 bytes, checksum: c01c4f087337cd82c64b06b90a1cc4dc (MD5)Made available in DSpace on 2017-01-17T18:55:36Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) sara_pacelli_sousa_marcial_parcial.pdf: 166236 bytes, checksum: c01c4f087337cd82c64b06b90a1cc4dc (MD5) Previous issue date: 2016Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais (FAPEMIG)Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Ci?ncias Farmac?uticas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, [2016].Paclitaxel (PTX) is a chemotherapeutic agent that plays an important role in the treatment of several types of human cancer, especially breast cancer. However, the low solubility of PTX in aqueous medium (partition coefficient log of 3.96) represents a barrier for intravenous administration. The conventional PTX formulation contains a high concentration of Cremophor-EL? (polyethoxylated castor oil), which is associated with significant toxicities restricting its clinical use. The encapsulation of the PTX in drug delivery systems could improve the uptake and increase its therapeutic efficacy. In this study, three different lipid nanosystems containing PTX, solid lipid nanoparticle (SLN), nanoemulsion (NE), and nanostructured lipid carrier (NLC) were prepared, and the physicochemical properties and in vitro cytotoxic activity were evaluated. Concerning the mean diameter, NLC blank showed diameter values approximately 2 and 1.7-fold lower than those obtained for SLN and NE, respectively. The presence of PTX leads to a significant increase in the particle diameter in all systems evaluated, except NE. In addition, increases in drug concentration (0.01% to 0.025%) produced an enhanced diameter for NLC preparation. All formulations containing PTX showed PI higher than 0.3, except for NE-PTX at drug concentration equal to 0.01% (w/v). Negative zeta potential values were observed in all formulations evaluated. NLC-PTX was the system more stable after storage for 30 days at 4 oC. The cytotoxicity studies on breast cancer cell lines (MDA-MB-231 and MCF-7) demonstrated cytotoxic activity more pronounced for NCL-PTX than for free PTX for both tumor cell lines. Thus, the results showed that NCL-PTX seems to be a potential tool for the treatment of breast cancer.porUFVJMA concess?o da licen?a deste item refere-se ao ? termo de autoriza??o impresso assinado pelo autor, assim como na licen?a Creative Commons, com as seguintes condi??es: Na qualidade de titular dos direitos de autor da publica??o, autorizo a Universidade Federal dos Vales do Jequitinhonha e Mucuri e o IBICT a disponibilizar por meio de seus reposit?rios, sem ressarcimento dos direitos autorais, de acordo com a Lei n? 9610/98, o texto parcial da obra disponibilizada, conforme permiss?es assinaladas, para fins de leitura, impress?o e/ou download, a t?tulo de divulga??o da produ??o cient?fica brasileira, e preserva??o, a partir desta data.info:eu-repo/semantics/openAccessDesenvolvimento de nanopart?culas lip?dicas contendo paclitaxelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPaclitaxelNanocarreadores lip?dicosC?ncerNanoemuls?oNanopart?cula lip?dica s?lidaCarreador lip?dico nanoestruturadoPaclitaxelLipid nanosystemsCancerNanoemulsionSolid lipid nanoparticleNanostructured lipid carrierreponame:Repositório Institucional da UFVJMinstname:Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)instacron:UFVJMTEXTsara_pacelli_sousa_marcial_parcial.pdf.txtsara_pacelli_sousa_marcial_parcial.pdf.txtExtracted texttext/plain26004http://acervo.ufvjm.edu.br/jspui/bitstream/1/1128/6/sara_pacelli_sousa_marcial_parcial.pdf.txt47d4882fb9698fd793b57d29db521945MD56LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Desenvolvimento de nanopart?culas lip?dicas contendo paclitaxel
title Desenvolvimento de nanopart?culas lip?dicas contendo paclitaxel
spellingShingle Desenvolvimento de nanopart?culas lip?dicas contendo paclitaxel
Marcial, Sara Pacelli de Sousa
Paclitaxel
Nanocarreadores lip?dicos
C?ncer
Nanoemuls?o
Nanopart?cula lip?dica s?lida
Carreador lip?dico nanoestruturado
Paclitaxel
Lipid nanosystems
Cancer
Nanoemulsion
Solid lipid nanoparticle
Nanostructured lipid carrier
title_short Desenvolvimento de nanopart?culas lip?dicas contendo paclitaxel
title_full Desenvolvimento de nanopart?culas lip?dicas contendo paclitaxel
title_fullStr Desenvolvimento de nanopart?culas lip?dicas contendo paclitaxel
title_full_unstemmed Desenvolvimento de nanopart?culas lip?dicas contendo paclitaxel
title_sort Desenvolvimento de nanopart?culas lip?dicas contendo paclitaxel
author Marcial, Sara Pacelli de Sousa
author_facet Marcial, Sara Pacelli de Sousa
author_role author
dc.contributor.references.none.fl_str_mv Leite, Elaine Amaral
Carneiro, Guilherme
Verly, Rodrigo Moreira
Carvalho Jr, ?lvaro Dutra de
dc.contributor.advisorco.none.fl_str_mv Carneiro, Guilherme
dc.contributor.institution.pt_BR.fl_str_mv Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
dc.contributor.author.fl_str_mv Marcial, Sara Pacelli de Sousa
dc.contributor.advisor1.fl_str_mv Leite, Elaine Amaral
contributor_str_mv Leite, Elaine Amaral
dc.subject.keyword.pt_BR.fl_str_mv Paclitaxel
Nanocarreadores lip?dicos
C?ncer
Nanoemuls?o
Nanopart?cula lip?dica s?lida
Carreador lip?dico nanoestruturado
topic Paclitaxel
Nanocarreadores lip?dicos
C?ncer
Nanoemuls?o
Nanopart?cula lip?dica s?lida
Carreador lip?dico nanoestruturado
Paclitaxel
Lipid nanosystems
Cancer
Nanoemulsion
Solid lipid nanoparticle
Nanostructured lipid carrier
dc.subject.keyword.en.fl_str_mv Paclitaxel
Lipid nanosystems
Cancer
Nanoemulsion
Solid lipid nanoparticle
Nanostructured lipid carrier
description ?rea de concentra??o: Ci?ncias farmac?uticas.
publishDate 2016
dc.date.submitted.none.fl_str_mv 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-01-17T18:55:36Z
dc.date.available.fl_str_mv 2017-01-17T18:55:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARCIAL, Sara Pacelli de Sousa. Desenvolvimento de nanopart?culas lip?dicas contendo paclitaxel. 2016. 98 p. Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Ci?ncias Farmac?uticas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2016.
dc.identifier.uri.fl_str_mv http://acervo.ufvjm.edu.br/jspui/handle/1/1128
identifier_str_mv MARCIAL, Sara Pacelli de Sousa. Desenvolvimento de nanopart?culas lip?dicas contendo paclitaxel. 2016. 98 p. Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Ci?ncias Farmac?uticas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, 2016.
url http://acervo.ufvjm.edu.br/jspui/handle/1/1128
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv UFVJM
publisher.none.fl_str_mv UFVJM
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFVJM
instname:Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
instacron:UFVJM
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reponame_str Repositório Institucional da UFVJM
collection Repositório Institucional da UFVJM
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