An integrated cytogenetic, flow and image cytometry procedure used to measure the DNA content of Zea mays A and B chromosomes

Detalhes bibliográficos
Autor(a) principal: Rosado, Tatiana Barbosa
Data de Publicação: 2008
Outros Autores: Clarindo, Wellington Ronildo, Carvalho, Carlos Roberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1016/j.plantsci.2008.10.007
http://www.locus.ufv.br/handle/123456789/22358
Resumo: The DNA content of some Zea mays chromosomes has been measured by flow karyotyping because similar chromosomes could not been discriminated. This technique requires suspensions with a high amount of intact chromosomes and protocols that provide enough resolution to quantify the DNA content of individual chromosomes. In order to measure the DNA content of A and B chromosomes of Z. mays ‘Black Mexican Sweet Corn’, we associated cytogenetic, flow and image cytometry tools. The cytogenetic methodology provided slides with A and B chromosomes adequate for karyogram assembly. The flow cytometry histograms exhibited distinct G0/G1 and G2 peaks for ‘Black Mexican Sweet Corn’ with (sample) and without (standard) B chromosomes. As a result, the nuclear DNA content of five plants showing B chromosomes was quantified. The nuclear genome size value was proportionally distributed according to the integrated optical density value, which was measured for individual chromosomes by image cytometry. Then, the DNA content of each A and B chromosome was calculated in picograms and base pairs. Based on qualitative (cytogenetic) and quantitative (flow and image cytometry) analyses, it was possible to discriminate and characterize the A and B chromosomes of ‘Black Mexican Sweet Corn’.
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spelling Rosado, Tatiana BarbosaClarindo, Wellington RonildoCarvalho, Carlos Roberto2018-10-19T11:32:01Z2018-10-19T11:32:01Z2008-10-3101689452https://doi.org/10.1016/j.plantsci.2008.10.007http://www.locus.ufv.br/handle/123456789/22358The DNA content of some Zea mays chromosomes has been measured by flow karyotyping because similar chromosomes could not been discriminated. This technique requires suspensions with a high amount of intact chromosomes and protocols that provide enough resolution to quantify the DNA content of individual chromosomes. In order to measure the DNA content of A and B chromosomes of Z. mays ‘Black Mexican Sweet Corn’, we associated cytogenetic, flow and image cytometry tools. The cytogenetic methodology provided slides with A and B chromosomes adequate for karyogram assembly. The flow cytometry histograms exhibited distinct G0/G1 and G2 peaks for ‘Black Mexican Sweet Corn’ with (sample) and without (standard) B chromosomes. As a result, the nuclear DNA content of five plants showing B chromosomes was quantified. The nuclear genome size value was proportionally distributed according to the integrated optical density value, which was measured for individual chromosomes by image cytometry. Then, the DNA content of each A and B chromosome was calculated in picograms and base pairs. Based on qualitative (cytogenetic) and quantitative (flow and image cytometry) analyses, it was possible to discriminate and characterize the A and B chromosomes of ‘Black Mexican Sweet Corn’.engPlant Sciencev. 176, n. 1, p. 154- 158, jan. 2009Elsevier Ireland Ltd.info:eu-repo/semantics/openAccessZea maysImage cytometryFlow cytometryCytogeneticB chromosomesChromosomal DNA contentAn integrated cytogenetic, flow and image cytometry procedure used to measure the DNA content of Zea mays A and B chromosomesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf323377https://locus.ufv.br//bitstream/123456789/22358/1/artigo.pdf0040bdae105311dd02f35ed82b7a0200MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/22358/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/223582018-10-19 08:35:27.721oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-10-19T11:35:27LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv An integrated cytogenetic, flow and image cytometry procedure used to measure the DNA content of Zea mays A and B chromosomes
title An integrated cytogenetic, flow and image cytometry procedure used to measure the DNA content of Zea mays A and B chromosomes
spellingShingle An integrated cytogenetic, flow and image cytometry procedure used to measure the DNA content of Zea mays A and B chromosomes
Rosado, Tatiana Barbosa
Zea mays
Image cytometry
Flow cytometry
Cytogenetic
B chromosomes
Chromosomal DNA content
title_short An integrated cytogenetic, flow and image cytometry procedure used to measure the DNA content of Zea mays A and B chromosomes
title_full An integrated cytogenetic, flow and image cytometry procedure used to measure the DNA content of Zea mays A and B chromosomes
title_fullStr An integrated cytogenetic, flow and image cytometry procedure used to measure the DNA content of Zea mays A and B chromosomes
title_full_unstemmed An integrated cytogenetic, flow and image cytometry procedure used to measure the DNA content of Zea mays A and B chromosomes
title_sort An integrated cytogenetic, flow and image cytometry procedure used to measure the DNA content of Zea mays A and B chromosomes
author Rosado, Tatiana Barbosa
author_facet Rosado, Tatiana Barbosa
Clarindo, Wellington Ronildo
Carvalho, Carlos Roberto
author_role author
author2 Clarindo, Wellington Ronildo
Carvalho, Carlos Roberto
author2_role author
author
dc.contributor.author.fl_str_mv Rosado, Tatiana Barbosa
Clarindo, Wellington Ronildo
Carvalho, Carlos Roberto
dc.subject.pt-BR.fl_str_mv Zea mays
Image cytometry
Flow cytometry
Cytogenetic
B chromosomes
Chromosomal DNA content
topic Zea mays
Image cytometry
Flow cytometry
Cytogenetic
B chromosomes
Chromosomal DNA content
description The DNA content of some Zea mays chromosomes has been measured by flow karyotyping because similar chromosomes could not been discriminated. This technique requires suspensions with a high amount of intact chromosomes and protocols that provide enough resolution to quantify the DNA content of individual chromosomes. In order to measure the DNA content of A and B chromosomes of Z. mays ‘Black Mexican Sweet Corn’, we associated cytogenetic, flow and image cytometry tools. The cytogenetic methodology provided slides with A and B chromosomes adequate for karyogram assembly. The flow cytometry histograms exhibited distinct G0/G1 and G2 peaks for ‘Black Mexican Sweet Corn’ with (sample) and without (standard) B chromosomes. As a result, the nuclear DNA content of five plants showing B chromosomes was quantified. The nuclear genome size value was proportionally distributed according to the integrated optical density value, which was measured for individual chromosomes by image cytometry. Then, the DNA content of each A and B chromosome was calculated in picograms and base pairs. Based on qualitative (cytogenetic) and quantitative (flow and image cytometry) analyses, it was possible to discriminate and characterize the A and B chromosomes of ‘Black Mexican Sweet Corn’.
publishDate 2008
dc.date.issued.fl_str_mv 2008-10-31
dc.date.accessioned.fl_str_mv 2018-10-19T11:32:01Z
dc.date.available.fl_str_mv 2018-10-19T11:32:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1016/j.plantsci.2008.10.007
http://www.locus.ufv.br/handle/123456789/22358
dc.identifier.issn.none.fl_str_mv 01689452
identifier_str_mv 01689452
url https://doi.org/10.1016/j.plantsci.2008.10.007
http://www.locus.ufv.br/handle/123456789/22358
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 176, n. 1, p. 154- 158, jan. 2009
dc.rights.driver.fl_str_mv Elsevier Ireland Ltd.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Elsevier Ireland Ltd.
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Plant Science
publisher.none.fl_str_mv Plant Science
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
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