Estrutura genética da população de Sclerotinia sclerotiorum em feijoais de Minas Gerais

• Autor(a) principal: Lehner, Miller da Silva
• Data de Publicação: 2011
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/4755
• topic: Diversidade genética; Mofo-branco do feijoeiro; Recombinação; Genetic diversity; White mold of bean; Recombination; CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS

Knowledge about the genetic structure of the population of Sclerotinia sclerotiorum can help decide the most adequate white mold management strategies, especially the development and use of resistant cultivars. The objective of this work was to study the genetic structure of populations of S. sclerotiorum on bean crops in Minas Gerais. We quantified the genetic variability of 127 isolates from four beanproducing regions (subpopulations), based on eight microsatellite markers. There was high genetic variability, with most variation due to differences among individuals within subpopulations. We identified 59 alleles and 65 haplotypes, with 49 haplotypes represented by a single isolate. There was no evidence of random mating in the subpopulations. Highest clonal fraction was recorded for the Zona da Mata subpopulation. The total population is moderately structured. However, there was no genetic differentiation between subpopulations of Noroeste, Zona da Mata, and Triângulo. However, all three subpopulations differed from that of Sul. Six cluster of isolates were detected, with evidence of immigrants among them. Most of the isolates from Sul subpopulation constituted a single group. In other groups there was mixing of individuals from other subpopulations. The high genetic variability observed in the population of S. sclerotiorum warns for greater care in the selection of bean genotypes resistant to white mold. The selection process should be performed in different regions of Minas Gerais in order to minimize the risk of supplanting resistance.