Exposure to spinosad induces histopathological and cytotoxic effects on the salivary complex of the non-target predator Podisus nigrispinus
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.chemosphere.2019.03.105 http://www.locus.ufv.br/handle/123456789/24301 |
Resumo: | In integrated pest management systems, biological and chemical controls must be compatible. The insecticide spinosad affects some non-target insects and might compromise their fitness. The objective of this study was to evaluate the histopathological and cytotoxic effects of spinosad on the salivary complex of the predatory bug Podisus nigrispinus (Heteroptera: Pentatomidae). Spinosad toxicity and insect survival were determined using six concentrations of insecticide. Ultrastructural changes and cell death of salivary glands were analyzed after P. nigrispinus exposure to spinosad LC50 (3.15 μg L−1). The insecticide caused toxicity to P. nigrispinus; survival was 32% after 48 h of exposure to LC50. The main histological changes in the salivary complex were disorganization of the epithelium, cytoplasmic vacuolization, and apocrine secretion into the gland lumen. Cytotoxic effects, such as release of granules and vacuoles into the lumen, presence of autophagosomes, enlargement of basal plasma membrane infoldings, and apoptosis, were observed. Spinosad causes toxicity, decreases survival, and changes the histology and cytology of the P. nigrispinus salivary complex. The results suggest that the cellular stress induced by the insecticide affects extra-oral digestion, compromising the potential of P. nigrispinus as a biological pest control agent. |
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Santos-Junior, Valdeir Celestino dosMartínez, Luis CarlosPlata-Rueda, AngelicaZanuncio, José ColaSerrão, José EduardoBozdoğan, Hakan2019-04-04T14:02:28Z2019-04-04T14:02:28Z2019-060045-6535https://doi.org/10.1016/j.chemosphere.2019.03.105http://www.locus.ufv.br/handle/123456789/24301In integrated pest management systems, biological and chemical controls must be compatible. The insecticide spinosad affects some non-target insects and might compromise their fitness. The objective of this study was to evaluate the histopathological and cytotoxic effects of spinosad on the salivary complex of the predatory bug Podisus nigrispinus (Heteroptera: Pentatomidae). Spinosad toxicity and insect survival were determined using six concentrations of insecticide. Ultrastructural changes and cell death of salivary glands were analyzed after P. nigrispinus exposure to spinosad LC50 (3.15 μg L−1). The insecticide caused toxicity to P. nigrispinus; survival was 32% after 48 h of exposure to LC50. The main histological changes in the salivary complex were disorganization of the epithelium, cytoplasmic vacuolization, and apocrine secretion into the gland lumen. Cytotoxic effects, such as release of granules and vacuoles into the lumen, presence of autophagosomes, enlargement of basal plasma membrane infoldings, and apoptosis, were observed. Spinosad causes toxicity, decreases survival, and changes the histology and cytology of the P. nigrispinus salivary complex. The results suggest that the cellular stress induced by the insecticide affects extra-oral digestion, compromising the potential of P. nigrispinus as a biological pest control agent.engChemosphereVolume 225, Pages 688-695, June 2019Elsevier B. V.info:eu-repo/semantics/openAccessApoptosisExtra-oral digestionNon-target organismSalivary glandToxicityExposure to spinosad induces histopathological and cytotoxic effects on the salivary complex of the non-target predator Podisus nigrispinusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf3043453https://locus.ufv.br//bitstream/123456789/24301/1/artigo.pdf8004860250095cf24b2a7f1ca270d60fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/24301/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/243012019-04-04 11:04:47.434oai:locus.ufv.br:123456789/24301Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-04-04T14:04:47LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Exposure to spinosad induces histopathological and cytotoxic effects on the salivary complex of the non-target predator Podisus nigrispinus |
title |
Exposure to spinosad induces histopathological and cytotoxic effects on the salivary complex of the non-target predator Podisus nigrispinus |
spellingShingle |
Exposure to spinosad induces histopathological and cytotoxic effects on the salivary complex of the non-target predator Podisus nigrispinus Santos-Junior, Valdeir Celestino dos Apoptosis Extra-oral digestion Non-target organism Salivary gland Toxicity |
title_short |
Exposure to spinosad induces histopathological and cytotoxic effects on the salivary complex of the non-target predator Podisus nigrispinus |
title_full |
Exposure to spinosad induces histopathological and cytotoxic effects on the salivary complex of the non-target predator Podisus nigrispinus |
title_fullStr |
Exposure to spinosad induces histopathological and cytotoxic effects on the salivary complex of the non-target predator Podisus nigrispinus |
title_full_unstemmed |
Exposure to spinosad induces histopathological and cytotoxic effects on the salivary complex of the non-target predator Podisus nigrispinus |
title_sort |
Exposure to spinosad induces histopathological and cytotoxic effects on the salivary complex of the non-target predator Podisus nigrispinus |
author |
Santos-Junior, Valdeir Celestino dos |
author_facet |
Santos-Junior, Valdeir Celestino dos Martínez, Luis Carlos Plata-Rueda, Angelica Zanuncio, José Cola Serrão, José Eduardo Bozdoğan, Hakan |
author_role |
author |
author2 |
Martínez, Luis Carlos Plata-Rueda, Angelica Zanuncio, José Cola Serrão, José Eduardo Bozdoğan, Hakan |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Santos-Junior, Valdeir Celestino dos Martínez, Luis Carlos Plata-Rueda, Angelica Zanuncio, José Cola Serrão, José Eduardo Bozdoğan, Hakan |
dc.subject.pt-BR.fl_str_mv |
Apoptosis Extra-oral digestion Non-target organism Salivary gland Toxicity |
topic |
Apoptosis Extra-oral digestion Non-target organism Salivary gland Toxicity |
description |
In integrated pest management systems, biological and chemical controls must be compatible. The insecticide spinosad affects some non-target insects and might compromise their fitness. The objective of this study was to evaluate the histopathological and cytotoxic effects of spinosad on the salivary complex of the predatory bug Podisus nigrispinus (Heteroptera: Pentatomidae). Spinosad toxicity and insect survival were determined using six concentrations of insecticide. Ultrastructural changes and cell death of salivary glands were analyzed after P. nigrispinus exposure to spinosad LC50 (3.15 μg L−1). The insecticide caused toxicity to P. nigrispinus; survival was 32% after 48 h of exposure to LC50. The main histological changes in the salivary complex were disorganization of the epithelium, cytoplasmic vacuolization, and apocrine secretion into the gland lumen. Cytotoxic effects, such as release of granules and vacuoles into the lumen, presence of autophagosomes, enlargement of basal plasma membrane infoldings, and apoptosis, were observed. Spinosad causes toxicity, decreases survival, and changes the histology and cytology of the P. nigrispinus salivary complex. The results suggest that the cellular stress induced by the insecticide affects extra-oral digestion, compromising the potential of P. nigrispinus as a biological pest control agent. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-04-04T14:02:28Z |
dc.date.available.fl_str_mv |
2019-04-04T14:02:28Z |
dc.date.issued.fl_str_mv |
2019-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.chemosphere.2019.03.105 http://www.locus.ufv.br/handle/123456789/24301 |
dc.identifier.issn.none.fl_str_mv |
0045-6535 |
identifier_str_mv |
0045-6535 |
url |
https://doi.org/10.1016/j.chemosphere.2019.03.105 http://www.locus.ufv.br/handle/123456789/24301 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
Volume 225, Pages 688-695, June 2019 |
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Elsevier B. V. info:eu-repo/semantics/openAccess |
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Elsevier B. V. |
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openAccess |
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Chemosphere |
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Chemosphere |
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