Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1590/0102-33062014abb0038 http://locus.ufv.br//handle/123456789/25822 |
Resumo: | Studies that evaluate the physiological and biochemical mechanisms of germination within forest species are needed in order to improve our understanding of such processes. Mercury and dithiothreitol are indicated as important tools in studies that assess the activity of aquaporins during imbibition and germination of seeds. To investigate the alterations caused by mercury inPlathymenia reticulata seedsdifferent doses of mercury were used in the presence and absence of dithiothreitol. Mercury had a dose-dependent effect on the seeds; in the most dilute solutions mercury partially inhibited the imbibition process, whereas in the most concentrated solutions it caused the death of the embryos. A delay in the hydration of the seeds may have caused decreased germination as a result of the reduced functionality of the aquaporins that were oxidized by mercury. In the presence of the reducing agent dithiothreitol, the activity of these proteins was restored and the germination process was re-established. These findings indicate the importance of aquaporins in the imbibition and germination stages of P. reticulataseeds, and they provide a better understanding of these important developmental events in plants. |
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Cardoso, Amanda ÁvilaBorges, Eduardo Euclydes de Lima eSouza, Genaina Aparecida deSilva, Cristiane Jovelina daPires, Raquel Maria de OliveiraDias, Denise Cunha Fernandes dos Santos2019-06-13T16:22:19Z2019-06-13T16:22:19Z2015-071677-941Xhttp://dx.doi.org/10.1590/0102-33062014abb0038http://locus.ufv.br//handle/123456789/25822Studies that evaluate the physiological and biochemical mechanisms of germination within forest species are needed in order to improve our understanding of such processes. Mercury and dithiothreitol are indicated as important tools in studies that assess the activity of aquaporins during imbibition and germination of seeds. To investigate the alterations caused by mercury inPlathymenia reticulata seedsdifferent doses of mercury were used in the presence and absence of dithiothreitol. Mercury had a dose-dependent effect on the seeds; in the most dilute solutions mercury partially inhibited the imbibition process, whereas in the most concentrated solutions it caused the death of the embryos. A delay in the hydration of the seeds may have caused decreased germination as a result of the reduced functionality of the aquaporins that were oxidized by mercury. In the presence of the reducing agent dithiothreitol, the activity of these proteins was restored and the germination process was re-established. These findings indicate the importance of aquaporins in the imbibition and germination stages of P. reticulataseeds, and they provide a better understanding of these important developmental events in plants.engActa Botanica Brasilicav. 29, n. 3, p. 285- 291, jul.- set. 2015AquaporinsDithiothreitolForest speciesMercurySeedSeed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporinsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf567358https://locus.ufv.br//bitstream/123456789/25822/1/artigo.pdf5ad6135c65eae2f96697c47122908e47MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/25822/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/258222019-06-13 15:23:09.967oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-06-13T18:23:09LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins |
title |
Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins |
spellingShingle |
Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins Cardoso, Amanda Ávila Aquaporins Dithiothreitol Forest species Mercury Seed |
title_short |
Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins |
title_full |
Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins |
title_fullStr |
Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins |
title_full_unstemmed |
Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins |
title_sort |
Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins |
author |
Cardoso, Amanda Ávila |
author_facet |
Cardoso, Amanda Ávila Borges, Eduardo Euclydes de Lima e Souza, Genaina Aparecida de Silva, Cristiane Jovelina da Pires, Raquel Maria de Oliveira Dias, Denise Cunha Fernandes dos Santos |
author_role |
author |
author2 |
Borges, Eduardo Euclydes de Lima e Souza, Genaina Aparecida de Silva, Cristiane Jovelina da Pires, Raquel Maria de Oliveira Dias, Denise Cunha Fernandes dos Santos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Cardoso, Amanda Ávila Borges, Eduardo Euclydes de Lima e Souza, Genaina Aparecida de Silva, Cristiane Jovelina da Pires, Raquel Maria de Oliveira Dias, Denise Cunha Fernandes dos Santos |
dc.subject.pt-BR.fl_str_mv |
Aquaporins Dithiothreitol Forest species Mercury Seed |
topic |
Aquaporins Dithiothreitol Forest species Mercury Seed |
description |
Studies that evaluate the physiological and biochemical mechanisms of germination within forest species are needed in order to improve our understanding of such processes. Mercury and dithiothreitol are indicated as important tools in studies that assess the activity of aquaporins during imbibition and germination of seeds. To investigate the alterations caused by mercury inPlathymenia reticulata seedsdifferent doses of mercury were used in the presence and absence of dithiothreitol. Mercury had a dose-dependent effect on the seeds; in the most dilute solutions mercury partially inhibited the imbibition process, whereas in the most concentrated solutions it caused the death of the embryos. A delay in the hydration of the seeds may have caused decreased germination as a result of the reduced functionality of the aquaporins that were oxidized by mercury. In the presence of the reducing agent dithiothreitol, the activity of these proteins was restored and the germination process was re-established. These findings indicate the importance of aquaporins in the imbibition and germination stages of P. reticulataseeds, and they provide a better understanding of these important developmental events in plants. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-07 |
dc.date.accessioned.fl_str_mv |
2019-06-13T16:22:19Z |
dc.date.available.fl_str_mv |
2019-06-13T16:22:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0102-33062014abb0038 http://locus.ufv.br//handle/123456789/25822 |
dc.identifier.issn.none.fl_str_mv |
1677-941X |
identifier_str_mv |
1677-941X |
url |
http://dx.doi.org/10.1590/0102-33062014abb0038 http://locus.ufv.br//handle/123456789/25822 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 29, n. 3, p. 285- 291, jul.- set. 2015 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Acta Botanica Brasilica |
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Acta Botanica Brasilica |
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