Adaptação do método rápido de Scharer para detecção da atividade de fosfatase alcalina residual em queijo Minas Padrão de acordo com as exigências internacionais

• Autor(a) principal: Martins, Fabiana de Oliveira
• Data de Publicação: 2006
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2893
• topic: Fosfatase alcalina; Queijo Minas Padrão; Pasteurização; Alkaline Phosphatase; Minas Padrão Cheese; Pasteurization; CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS

In 2003, in the United States, the Food and Drug Administration established as legal limit the activity of 350 mU/Kg for milk products, and European legislations that adopted this limit for milk, study the change for other milk products. This research evaluated the sensibility and the linearity response of the fluorimetric and Rapid Scharer s methods in the determination of the of residual alkaline phosphatase (ALP) activity in Minas Padrão cheese. Another purpose was to develop an analytic methodology (Adapted Rapid Scharer s method) for detection of residual ALP activity in Minas Padrão cheese with sensibility corresponding of the fluorimetric method in the activity of 350 mU/Kg, however with smaller cost. Was appraised the residual ALP activity in commercial samples of this cheese type, verifying the acting of Adapted Rapid Scharer s method. The fluorimetric method presented appropriate in the determination of residual ALP activity in Minas Padrão cheese in agreement with the FDA limit. The same cannot be said for the Rapid Scharer s method, that detected as positive only samples with concentrations of raw milk added to the milk pasteurized larger to 0,5 % (activity corresponding to 8.849±797 mU/Kg and 2 µg of cheese fenol/g). The Adapted Rapid Scharer s method presented excellent correlation with the fluorimetric method in the strip of activity of 350 mU/Kg, and samples with approximate activity of 350 mU/Kg (corresponding to 0,010 %) were detected as positive. In commercial Minas Padrão cheese, was verified, by the fluorimetric method, that 81,25 % of the samples had larger activities to 350 mU/Kg. The same result was obtained by the Adapted Rapid Scharer s. Therefore, to evaluate the efficiency of the pasteurization of the milk destined to the production of Minas Padrão cheese isn t advisable the use of the Rapid Scharer s Method. The fluorimetric method is appropriate and is suggested your legalization in Brazil. The Adapted Rapid Scharer s appears as efficient alternative, of accessible cost, for determination of residual ALP activity in Minas Padrão cheese, in agreement with the level of international demand established for FDA. It s suggested that the quantitative criterion be adopted in Brazil.