Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum

Detalhes bibliográficos
Autor(a) principal: Ribon, A. O. B.
Data de Publicação: 1999
Outros Autores: Coelho, J. L. C., Barros, E. G. de, Araújo, E. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1023/A:1005419225412
http://www.locus.ufv.br/handle/123456789/22655
Resumo: A conserved region of a polygalacturonase (PG) gene from Penicillium griseoroseum was PCR amplified and used to screen a genomic library from this fungus. The nucleotide sequence of the isolated clone (pggI) consisted of 1497 bp, including a coding region of 1251 bp. This region potentially encodes a protein of 376 amino acids, and is interrupted by two introns. Extensive homology was observed between this protein and several fungal endopolygalacturonases. DNA hybridization analyses revealed that there is a low copy number of pggI in the P. griseoroseum genome, probably one or two copies.
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spelling Ribon, A. O. B.Coelho, J. L. C.Barros, E. G. deAraújo, E. F.2018-11-29T17:14:44Z2018-11-29T17:14:44Z1999-0515736776http://dx.doi.org/10.1023/A:1005419225412http://www.locus.ufv.br/handle/123456789/22655A conserved region of a polygalacturonase (PG) gene from Penicillium griseoroseum was PCR amplified and used to screen a genomic library from this fungus. The nucleotide sequence of the isolated clone (pggI) consisted of 1497 bp, including a coding region of 1251 bp. This region potentially encodes a protein of 376 amino acids, and is interrupted by two introns. Extensive homology was observed between this protein and several fungal endopolygalacturonases. DNA hybridization analyses revealed that there is a low copy number of pggI in the P. griseoroseum genome, probably one or two copies.engBiotechnology LettersVolume 21, Issue 5, Pages 395– 399, May 19991999 Kluwer Academic Publishers. Printed in the Netherlands.info:eu-repo/semantics/openAccessFilamentous fungiGene cloningPectinaseSequence homologyCloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf122603https://locus.ufv.br//bitstream/123456789/22655/1/artigo.pdf1926e5a3967ac21c13a126753458245eMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/22655/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/226552018-11-29 15:52:12.07oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-11-29T18:52:12LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum
title Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum
spellingShingle Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum
Ribon, A. O. B.
Filamentous fungi
Gene cloning
Pectinase
Sequence homology
title_short Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum
title_full Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum
title_fullStr Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum
title_full_unstemmed Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum
title_sort Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum
author Ribon, A. O. B.
author_facet Ribon, A. O. B.
Coelho, J. L. C.
Barros, E. G. de
Araújo, E. F.
author_role author
author2 Coelho, J. L. C.
Barros, E. G. de
Araújo, E. F.
author2_role author
author
author
dc.contributor.author.fl_str_mv Ribon, A. O. B.
Coelho, J. L. C.
Barros, E. G. de
Araújo, E. F.
dc.subject.pt-BR.fl_str_mv Filamentous fungi
Gene cloning
Pectinase
Sequence homology
topic Filamentous fungi
Gene cloning
Pectinase
Sequence homology
description A conserved region of a polygalacturonase (PG) gene from Penicillium griseoroseum was PCR amplified and used to screen a genomic library from this fungus. The nucleotide sequence of the isolated clone (pggI) consisted of 1497 bp, including a coding region of 1251 bp. This region potentially encodes a protein of 376 amino acids, and is interrupted by two introns. Extensive homology was observed between this protein and several fungal endopolygalacturonases. DNA hybridization analyses revealed that there is a low copy number of pggI in the P. griseoroseum genome, probably one or two copies.
publishDate 1999
dc.date.issued.fl_str_mv 1999-05
dc.date.accessioned.fl_str_mv 2018-11-29T17:14:44Z
dc.date.available.fl_str_mv 2018-11-29T17:14:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1023/A:1005419225412
http://www.locus.ufv.br/handle/123456789/22655
dc.identifier.issn.none.fl_str_mv 15736776
identifier_str_mv 15736776
url http://dx.doi.org/10.1023/A:1005419225412
http://www.locus.ufv.br/handle/123456789/22655
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv Volume 21, Issue 5, Pages 395– 399, May 1999
dc.rights.driver.fl_str_mv 1999 Kluwer Academic Publishers. Printed in the Netherlands.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv 1999 Kluwer Academic Publishers. Printed in the Netherlands.
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Biotechnology Letters
publisher.none.fl_str_mv Biotechnology Letters
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
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