Avaliação da ação do fungo predador de nematóide Duddingtonia flagrans sobre larvas infectantes de Ancylostoma ceylanicum

• Autor(a) principal: Fernandes, Fernanda Mara
• Data de Publicação: 2011
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/5069
• topic: Controle biológico; Ancylostoma ceylanicum; Biological control; Ancylostoma ceylanicum; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA

Ancylostoma ceylanicum is a gastrointestinal nematode of dogs and cats, can parasitize humans. Is important to use alternative ways that can be used to fighting the spread of these parasites and their environmental infective forms, such as the use of natural antagonists of nematodes The biological control with nematophagous fungi has been suggested as an alternative to reduce this and other gastrointestinal infections caused by nematodes. The genus Duddingtonia stands out by control of nematodes in the environment. Duddingtonia flagrans is considered as the most promising species, due to the abundance of chlamydospores, which are structures of resistance. This study evaluated a fungus isolated from D. flagrans (AC001) in vitro for their ability to prey infective L3 larvae of A. ceylanicum, and its effectiveness in capturing larvae. Then, evaluated the ability to withstand the passage through the gastrointestinal tract of hamsters and ability to prey on the larvae. Was assessed on the dose of the mycelium would be more effective in relapse of the infection, were used 50 hamsters that received orally 100 L3 of A. ceylanicum. After, were formed five groups of 10 animals each, receiving fungal mycelia (25grams by weight) for a period of 14 days, every two days, and four groups treated with the fungus D. flagrans in different concentrations: group 1 (0.04 grams), group 2 (0.08 grams), group 3 (0.16 grams), group 4 (0.32 grams) and a control group that don t received this treatment. The animals were placed in separate cages and after the period of patency by A.ceylanicum (20 days) every two days, 2 grams of faeces from each group were collected from beds of hamsters for further proceed with the count of eggs per gram of feces (OPG).The results showed that there was difference (p<0.05) in reduction of the OPG of treated groups when compared to the control group.There was no difference (p>0.05) in reducing the OPG in the treated groups at different concentrations. The results of this study suggest that the fungus D. flagrans could be used as a tool in biological control of A. ceylanicum in natural conditions.