Xanthomonas axonopodis pv. eucalyptorum pv. nov. causing bacterial leaf blight on eucalypt in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.5423/PPJ.OA.01.2018.0014 http://www.locus.ufv.br/handle/123456789/23076 |
Resumo: | Bacterial leaf blight is a major disease of eucalypt, especially under nursery conditions. Different bacterial species have been associated with the disease in several countries, and despite its importance worldwide, it is not clear to date whether similar disease symptoms are caused by the same or by different etiological agents. In this study, 43 bacterial strains were isolated from blighted eucalypt leaves collected in different geographic areas of Brazil and inoculated onto a susceptible eucalypt clone. Polyphasic taxonomy, including morphological, physiological, biochemical, molecular, and pathogenicity tests showed that only certain strains of Xanthomonas axonopodis caused symptoms of the disease. Strains varied in their aggressiveness, but no correlation with geographic origin was observed. MLSA-based phylogenetic analysis using concatenated dnaK, fyuA, gyrB and rpoD gene sequences allocated the strains in a well-defined clade, corresponding to Rade-marker’s group RG 9.6. Inoculation of nineteen plant species belonging to seven botanical families with representative strain LPF 602 showed it to be pathogenic only on Eucalyptus spp, and Corymbia spp. Based on distinct biochemical and pathogenic characteristics that differentiate the eucalypt strains from other pathovars of the X. axonopodis species, here we propose their allocation into the new pathovar X. axonopodis pv. eucalyptorum pv. nov. |
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Ferraz, Hélvio Gledson MacielBadel, Jorge LuisGuimarães, Lúcio Mauro da SilvaReis, Bruna PaolinelliTótola, Marcos RogérioGonçalves, Rivadalve CoelhoAlfenas, Acelino Couto2019-01-17T15:56:46Z2019-01-17T15:56:46Z2018-082093-9280http://dx.doi.org/10.5423/PPJ.OA.01.2018.0014http://www.locus.ufv.br/handle/123456789/23076Bacterial leaf blight is a major disease of eucalypt, especially under nursery conditions. Different bacterial species have been associated with the disease in several countries, and despite its importance worldwide, it is not clear to date whether similar disease symptoms are caused by the same or by different etiological agents. In this study, 43 bacterial strains were isolated from blighted eucalypt leaves collected in different geographic areas of Brazil and inoculated onto a susceptible eucalypt clone. Polyphasic taxonomy, including morphological, physiological, biochemical, molecular, and pathogenicity tests showed that only certain strains of Xanthomonas axonopodis caused symptoms of the disease. Strains varied in their aggressiveness, but no correlation with geographic origin was observed. MLSA-based phylogenetic analysis using concatenated dnaK, fyuA, gyrB and rpoD gene sequences allocated the strains in a well-defined clade, corresponding to Rade-marker’s group RG 9.6. Inoculation of nineteen plant species belonging to seven botanical families with representative strain LPF 602 showed it to be pathogenic only on Eucalyptus spp, and Corymbia spp. Based on distinct biochemical and pathogenic characteristics that differentiate the eucalypt strains from other pathovars of the X. axonopodis species, here we propose their allocation into the new pathovar X. axonopodis pv. eucalyptorum pv. nov.engThe Plant Pathology JournalV. 34, N. 4, P. 269– 285, Aug. 2018Eucalyptus spp.Fatty acid profileMetabolism fingerprintingMultilocus sequence analysisXanthomonas axonopodisXanthomonas axonopodis pv. eucalyptorum pv. nov. causing bacterial leaf blight on eucalypt in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf1928399https://locus.ufv.br//bitstream/123456789/23076/1/artigo.pdf2d0715b9f47e70e3cbfb4a41e638c2d2MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/23076/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/230762019-01-17 14:37:49.068oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-01-17T17:37:49LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Xanthomonas axonopodis pv. eucalyptorum pv. nov. causing bacterial leaf blight on eucalypt in Brazil |
title |
Xanthomonas axonopodis pv. eucalyptorum pv. nov. causing bacterial leaf blight on eucalypt in Brazil |
spellingShingle |
Xanthomonas axonopodis pv. eucalyptorum pv. nov. causing bacterial leaf blight on eucalypt in Brazil Ferraz, Hélvio Gledson Maciel Eucalyptus spp. Fatty acid profile Metabolism fingerprinting Multilocus sequence analysis Xanthomonas axonopodis |
title_short |
Xanthomonas axonopodis pv. eucalyptorum pv. nov. causing bacterial leaf blight on eucalypt in Brazil |
title_full |
Xanthomonas axonopodis pv. eucalyptorum pv. nov. causing bacterial leaf blight on eucalypt in Brazil |
title_fullStr |
Xanthomonas axonopodis pv. eucalyptorum pv. nov. causing bacterial leaf blight on eucalypt in Brazil |
title_full_unstemmed |
Xanthomonas axonopodis pv. eucalyptorum pv. nov. causing bacterial leaf blight on eucalypt in Brazil |
title_sort |
Xanthomonas axonopodis pv. eucalyptorum pv. nov. causing bacterial leaf blight on eucalypt in Brazil |
author |
Ferraz, Hélvio Gledson Maciel |
author_facet |
Ferraz, Hélvio Gledson Maciel Badel, Jorge Luis Guimarães, Lúcio Mauro da Silva Reis, Bruna Paolinelli Tótola, Marcos Rogério Gonçalves, Rivadalve Coelho Alfenas, Acelino Couto |
author_role |
author |
author2 |
Badel, Jorge Luis Guimarães, Lúcio Mauro da Silva Reis, Bruna Paolinelli Tótola, Marcos Rogério Gonçalves, Rivadalve Coelho Alfenas, Acelino Couto |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Ferraz, Hélvio Gledson Maciel Badel, Jorge Luis Guimarães, Lúcio Mauro da Silva Reis, Bruna Paolinelli Tótola, Marcos Rogério Gonçalves, Rivadalve Coelho Alfenas, Acelino Couto |
dc.subject.pt-BR.fl_str_mv |
Eucalyptus spp. Fatty acid profile Metabolism fingerprinting Multilocus sequence analysis Xanthomonas axonopodis |
topic |
Eucalyptus spp. Fatty acid profile Metabolism fingerprinting Multilocus sequence analysis Xanthomonas axonopodis |
description |
Bacterial leaf blight is a major disease of eucalypt, especially under nursery conditions. Different bacterial species have been associated with the disease in several countries, and despite its importance worldwide, it is not clear to date whether similar disease symptoms are caused by the same or by different etiological agents. In this study, 43 bacterial strains were isolated from blighted eucalypt leaves collected in different geographic areas of Brazil and inoculated onto a susceptible eucalypt clone. Polyphasic taxonomy, including morphological, physiological, biochemical, molecular, and pathogenicity tests showed that only certain strains of Xanthomonas axonopodis caused symptoms of the disease. Strains varied in their aggressiveness, but no correlation with geographic origin was observed. MLSA-based phylogenetic analysis using concatenated dnaK, fyuA, gyrB and rpoD gene sequences allocated the strains in a well-defined clade, corresponding to Rade-marker’s group RG 9.6. Inoculation of nineteen plant species belonging to seven botanical families with representative strain LPF 602 showed it to be pathogenic only on Eucalyptus spp, and Corymbia spp. Based on distinct biochemical and pathogenic characteristics that differentiate the eucalypt strains from other pathovars of the X. axonopodis species, here we propose their allocation into the new pathovar X. axonopodis pv. eucalyptorum pv. nov. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-08 |
dc.date.accessioned.fl_str_mv |
2019-01-17T15:56:46Z |
dc.date.available.fl_str_mv |
2019-01-17T15:56:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5423/PPJ.OA.01.2018.0014 http://www.locus.ufv.br/handle/123456789/23076 |
dc.identifier.issn.none.fl_str_mv |
2093-9280 |
identifier_str_mv |
2093-9280 |
url |
http://dx.doi.org/10.5423/PPJ.OA.01.2018.0014 http://www.locus.ufv.br/handle/123456789/23076 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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V. 34, N. 4, P. 269– 285, Aug. 2018 |
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openAccess |
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The Plant Pathology Journal |
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The Plant Pathology Journal |
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