A proteína BiP exerce função protetora contra o déficit hídrico em plantas de soja

Detalhes bibliográficos
Autor(a) principal: Valente, Maria Anete Santana
Data de Publicação: 2008
Tipo de documento: Tese
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/324
Resumo: The plants are under continuous biotic and abiotic stress conditions, and drought is the major abiotic factors which limits agricultural production, triggering biochemical, physiological and molecular changes in plants. Based on the capacity of BiP to increase drought tolerance in Nicotiana tabacum, it was aimed in this research to analyze if such molecular strategy for increasing the tolerance would be effective in soybean. Soybean plants, belonging to the consquista variety, were initially transformed with the soyBiPD gene under control of the 35S constitutive promoter. The transgenic analysis was evaluated in segregating populations until the T6 generation. The transgene expression was analyzed in six transformants called 35S:BiP-1, 35S:BiP-2, 35S:BiP-3, 35S:BiP-4, 35S:BiP-5, 35S:BiP-6. On T6 generation, homozygous plants of the 35S:BiP-4 lineage that were overexpressing BiP were selected. Higher BiP protein accumulation was found in the endoplasmatic reticulum of the transgenic plants, indicating a right cellular addressing of the recombinant protein. T3 soybean plants overexpressing BiP were subjected to hydric stress. After hydric restriction, a tolerant phenotype was observed in plants sense which kept leaf turgescence level without accumulating high levels of sucrose, while soybean control plants did not have the same leaf turgescence level and they accumulated higher levels of sucrose. Under hydric restriction, transformed plants significantly showed higher photosynthetic ability and stomatic conductance than non-transformed plants. T4 and T5 soybean plants were also subjected to severe hydric deficit by complete watering interruption and the transgenic plants revealed higher drought resistance. Nontransformed plants significantly showed lower photosynthetic, stomatic conductance and transpiration rates in this water stress. Proline concentration increased significantly in plants that underwent drought, being noticeably higher in the wild type plants. The stress condition increased malondialdehyde (MDA) contents in soybean plants, occurring peroxidation in the membrane lipids of both plants, but it was significantly higher in the controls. It was also found that control plant roots under osmotic stress showed greater branching. Under conditions of water stress, genes were differentially expressed in transgenic lineages and control plants, standing out the induction of drought responsive genes, such as LEA, antiquitin and GST, in non-transformed plants. BiP superexpression delayed leaf senescence induction, being chlorophyll and protein percentages significantly lower in the control plants. Altogether, these results suggest that BiP protein has a protector function against the water deficit in plants.
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spelling Valente, Maria Anete Santanahttp://lattes.cnpq.br/7809094025770798Aragão, Francisco José Limahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785487A7Loureiro, Marcelo Ehlershttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780851Y3Fontes, Elizabeth Pacheco Batistahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781848H2Fietto, Luciano Gomeshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763824H8Pereira, Maria Cristina Baracathttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780021E62015-03-26T12:15:21Z2013-09-132015-03-26T12:15:21Z2008-07-23VALENTE, Maria Anete Santana. The protein BiP has a protector function against the water deficit in plants of soybean. 2008. 122 f. Tese (Doutorado em Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal) - Universidade Federal de Viçosa, Viçosa, 2008.http://locus.ufv.br/handle/123456789/324The plants are under continuous biotic and abiotic stress conditions, and drought is the major abiotic factors which limits agricultural production, triggering biochemical, physiological and molecular changes in plants. Based on the capacity of BiP to increase drought tolerance in Nicotiana tabacum, it was aimed in this research to analyze if such molecular strategy for increasing the tolerance would be effective in soybean. Soybean plants, belonging to the consquista variety, were initially transformed with the soyBiPD gene under control of the 35S constitutive promoter. The transgenic analysis was evaluated in segregating populations until the T6 generation. The transgene expression was analyzed in six transformants called 35S:BiP-1, 35S:BiP-2, 35S:BiP-3, 35S:BiP-4, 35S:BiP-5, 35S:BiP-6. On T6 generation, homozygous plants of the 35S:BiP-4 lineage that were overexpressing BiP were selected. Higher BiP protein accumulation was found in the endoplasmatic reticulum of the transgenic plants, indicating a right cellular addressing of the recombinant protein. T3 soybean plants overexpressing BiP were subjected to hydric stress. After hydric restriction, a tolerant phenotype was observed in plants sense which kept leaf turgescence level without accumulating high levels of sucrose, while soybean control plants did not have the same leaf turgescence level and they accumulated higher levels of sucrose. Under hydric restriction, transformed plants significantly showed higher photosynthetic ability and stomatic conductance than non-transformed plants. T4 and T5 soybean plants were also subjected to severe hydric deficit by complete watering interruption and the transgenic plants revealed higher drought resistance. Nontransformed plants significantly showed lower photosynthetic, stomatic conductance and transpiration rates in this water stress. Proline concentration increased significantly in plants that underwent drought, being noticeably higher in the wild type plants. The stress condition increased malondialdehyde (MDA) contents in soybean plants, occurring peroxidation in the membrane lipids of both plants, but it was significantly higher in the controls. It was also found that control plant roots under osmotic stress showed greater branching. Under conditions of water stress, genes were differentially expressed in transgenic lineages and control plants, standing out the induction of drought responsive genes, such as LEA, antiquitin and GST, in non-transformed plants. BiP superexpression delayed leaf senescence induction, being chlorophyll and protein percentages significantly lower in the control plants. Altogether, these results suggest that BiP protein has a protector function against the water deficit in plants.As plantas estão sob constantes condições de estresses bióticos e abióticos, e a seca é um dos principais fatores abióticos limitantes da produção agrícola, desencadeando alterações bioquímicas, fisiológicas e moleculares nas plantas. Baseada na capacidade de BiP em aumentar tolerância à seca em Nicotiana tabacum, propôs-se analisar se esta estratégia molecular para aumento de tolerância seria efetiva em soja. Inicialmente, plantas de soja, variedade ‘Conquista’, foram transformadas com o gene soyBiPD, sob o controle do promotor constitutivo 35S e a análise de transgenia foi avaliada em populações segregantes até a geração T6. A expressão do transgene foi analisada em seis transformantes, designados 35S:BiP-1, 35S:BiP-2, 35S:BiP-3, 35S:BiP-4, 35S:BiP-5, 35S:BiP-6. Na geração T6, foram selecionadas plantas homozigotas, superexpressando BiP, da linhagem 35S:BiP-4. Maior acúmulo da proteína BiP foi observado no retículo endoplasmático das plantas transgênicas, indicando um correto endereçamento celular da proteína recombinante. Plantas de soja T3 superexpressando BiP foram submetidas a condições de estresse hídrico. Após condição de restrição hídrica, foi observado um fenótipo tolerante nas plantas senso com manutenção do nível de turgescência foliar e não acumularam níveis elevados de sacarose, enquanto as plantas de soja controle não mantiveram o mesmo nível de turgescência foliar e acumularam maiores níveis de sacarose. Sob condições de restrição hídrica, as plantas senso apresentaram capacidade fotossintética e de condutância estomática significativamente superiores às plantas não transformadas. Plantas de soja T4 e T5 também foram submetidas a déficit hídrico severo pela suspensão total de irrigação e as plantas transgênicas mostraram maior tolerância à seca. As plantas não transformadas apresentaram taxa fotossintética, condutância estomática e transpiração significativamente inferiores nessas condições. As concentrações de prolina aumentaram significativamente nas plantas sob seca, apresentando-se significativamente superior nas plantas wild type. A condição de estresse elevou o conteúdo de malondialdeído (MDA) nas plantas de soja, ocorrendo peroxidação de lipídios em ambas, mas significativamente superior nas plantas controle. Foi constatado também que as raízes das plantas controle, sob estresse osmótico, apresentaram ramificação maior. Em condições de estresse hídrico genes foram diferencialmente expressos nas linhagens transgênicas e nas plantas controle, destacando a indução superior de genes responsivos à seca, como genes LEA, antiquitina e GST nas plantas não transformadas. A superexpressão de BiP retardou a indução do processo de senescência foliar, sendo o teor de clorofila e de proteína significativamente inferiores nas plantas controle. Coletivamente, estes resultados sugerem que a proteína BiP exerce função protetora contra o déficit hídrico em plantas.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaDoutorado em Bioquímica AgrícolaUFVBRBioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animalBIPSojaDéficit hídricoBIPSoybeanWater deficitCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULARA proteína BiP exerce função protetora contra o déficit hídrico em plantas de sojaThe protein BiP has a protector function against the water deficit in plants of soybeaninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf3858802https://locus.ufv.br//bitstream/123456789/324/1/texto%20completo.pdfc71f6de3a2325d80a234b24a7b6314b4MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain161083https://locus.ufv.br//bitstream/123456789/324/2/texto%20completo.pdf.txt3ef30bb2c5b4fcace56c821ef5f33ac2MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3525https://locus.ufv.br//bitstream/123456789/324/3/texto%20completo.pdf.jpgd8f3c3ebe29ee8a6336624834a956a2fMD53123456789/3242016-04-06 23:01:15.652oai:locus.ufv.br:123456789/324Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-07T02:01:15LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv A proteína BiP exerce função protetora contra o déficit hídrico em plantas de soja
dc.title.alternative.eng.fl_str_mv The protein BiP has a protector function against the water deficit in plants of soybean
title A proteína BiP exerce função protetora contra o déficit hídrico em plantas de soja
spellingShingle A proteína BiP exerce função protetora contra o déficit hídrico em plantas de soja
Valente, Maria Anete Santana
BIP
Soja
Déficit hídrico
BIP
Soybean
Water deficit
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR
title_short A proteína BiP exerce função protetora contra o déficit hídrico em plantas de soja
title_full A proteína BiP exerce função protetora contra o déficit hídrico em plantas de soja
title_fullStr A proteína BiP exerce função protetora contra o déficit hídrico em plantas de soja
title_full_unstemmed A proteína BiP exerce função protetora contra o déficit hídrico em plantas de soja
title_sort A proteína BiP exerce função protetora contra o déficit hídrico em plantas de soja
author Valente, Maria Anete Santana
author_facet Valente, Maria Anete Santana
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/7809094025770798
dc.contributor.author.fl_str_mv Valente, Maria Anete Santana
dc.contributor.advisor-co1.fl_str_mv Aragão, Francisco José Lima
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785487A7
dc.contributor.advisor-co2.fl_str_mv Loureiro, Marcelo Ehlers
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780851Y3
dc.contributor.advisor1.fl_str_mv Fontes, Elizabeth Pacheco Batista
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781848H2
dc.contributor.referee1.fl_str_mv Fietto, Luciano Gomes
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763824H8
dc.contributor.referee2.fl_str_mv Pereira, Maria Cristina Baracat
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780021E6
contributor_str_mv Aragão, Francisco José Lima
Loureiro, Marcelo Ehlers
Fontes, Elizabeth Pacheco Batista
Fietto, Luciano Gomes
Pereira, Maria Cristina Baracat
dc.subject.por.fl_str_mv BIP
Soja
Déficit hídrico
topic BIP
Soja
Déficit hídrico
BIP
Soybean
Water deficit
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR
dc.subject.eng.fl_str_mv BIP
Soybean
Water deficit
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR
description The plants are under continuous biotic and abiotic stress conditions, and drought is the major abiotic factors which limits agricultural production, triggering biochemical, physiological and molecular changes in plants. Based on the capacity of BiP to increase drought tolerance in Nicotiana tabacum, it was aimed in this research to analyze if such molecular strategy for increasing the tolerance would be effective in soybean. Soybean plants, belonging to the consquista variety, were initially transformed with the soyBiPD gene under control of the 35S constitutive promoter. The transgenic analysis was evaluated in segregating populations until the T6 generation. The transgene expression was analyzed in six transformants called 35S:BiP-1, 35S:BiP-2, 35S:BiP-3, 35S:BiP-4, 35S:BiP-5, 35S:BiP-6. On T6 generation, homozygous plants of the 35S:BiP-4 lineage that were overexpressing BiP were selected. Higher BiP protein accumulation was found in the endoplasmatic reticulum of the transgenic plants, indicating a right cellular addressing of the recombinant protein. T3 soybean plants overexpressing BiP were subjected to hydric stress. After hydric restriction, a tolerant phenotype was observed in plants sense which kept leaf turgescence level without accumulating high levels of sucrose, while soybean control plants did not have the same leaf turgescence level and they accumulated higher levels of sucrose. Under hydric restriction, transformed plants significantly showed higher photosynthetic ability and stomatic conductance than non-transformed plants. T4 and T5 soybean plants were also subjected to severe hydric deficit by complete watering interruption and the transgenic plants revealed higher drought resistance. Nontransformed plants significantly showed lower photosynthetic, stomatic conductance and transpiration rates in this water stress. Proline concentration increased significantly in plants that underwent drought, being noticeably higher in the wild type plants. The stress condition increased malondialdehyde (MDA) contents in soybean plants, occurring peroxidation in the membrane lipids of both plants, but it was significantly higher in the controls. It was also found that control plant roots under osmotic stress showed greater branching. Under conditions of water stress, genes were differentially expressed in transgenic lineages and control plants, standing out the induction of drought responsive genes, such as LEA, antiquitin and GST, in non-transformed plants. BiP superexpression delayed leaf senescence induction, being chlorophyll and protein percentages significantly lower in the control plants. Altogether, these results suggest that BiP protein has a protector function against the water deficit in plants.
publishDate 2008
dc.date.issued.fl_str_mv 2008-07-23
dc.date.available.fl_str_mv 2013-09-13
2015-03-26T12:15:21Z
dc.date.accessioned.fl_str_mv 2015-03-26T12:15:21Z
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dc.identifier.citation.fl_str_mv VALENTE, Maria Anete Santana. The protein BiP has a protector function against the water deficit in plants of soybean. 2008. 122 f. Tese (Doutorado em Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal) - Universidade Federal de Viçosa, Viçosa, 2008.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/324
identifier_str_mv VALENTE, Maria Anete Santana. The protein BiP has a protector function against the water deficit in plants of soybean. 2008. 122 f. Tese (Doutorado em Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal) - Universidade Federal de Viçosa, Viçosa, 2008.
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