Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | LOCUS Repositório Institucional da UFV |
| Texto Completo: | http://dx.doi.org/10.3389/fpls.2013.00308 http://www.locus.ufv.br/handle/123456789/17823 |
Resumo: | O artigo não contém o resumo em português. |
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Nunes-Nesi, AdrianoOsorio, SoniaStratmann, MarinaFernie, Alisdair R.2018-02-22T11:17:35Z2018-02-22T11:17:35Z2013-08-091664462Xhttp://dx.doi.org/10.3389/fpls.2013.00308http://www.locus.ufv.br/handle/123456789/17823O artigo não contém o resumo em português.Ascorbate (vitamin C) deficiency leads to low immunity, scurvy, and other human diseases and is therefore a global health problem. Given that plants are major ascorbate sources for humans, biofortification of this vitamin in our foodstuffs is of considerable importance. Ascorbate is synthetized by a number of alternative pathways: (i) from the glycolytic intermediates D-glucose-6P (the key intermediates are GDP-D-mannose and L-galactose), (ii) from the breakdown of the cell wall polymer pectin which uses the methyl ester of D-galacturonic acid as precursor, and (iii) from myo-inositol as precursor via myo-inositol oxygenase. We report here the engineering of fruit-specific overexpression of a bacterial pyrophosphatase, which hydrolyzes the inorganic pyrophosphate (PPi) to orthophosphate (Pi). This strategy resulted in increased vitamin C levels up to 2.5-fold in ripe fruit as well as increasing in the major sugars, sucrose, and glucose, yet decreasing the level of starch. When considered together, these finding indicate an intimate linkage between ascorbate and sugar biosynthesis in plants. Moreover, the combined data reveal the importance of PPi metabolism in tomato fruit metabolism and development.engFrontiers in Plant Sciencev. 4, Article 308, p. 01-13, Agosto 2013Tomato fruitRipeningAscorbateSugarsPyrophosphatasePyrophosphate levels strongly influence ascorbate and starch content in tomato fruitinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf2431215https://locus.ufv.br//bitstream/123456789/17823/1/artigo.pdfbad3cefe414d584d427effb9e7140f0cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/17823/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5114https://locus.ufv.br//bitstream/123456789/17823/3/artigo.pdf.jpga8ce64a4da13edd5066454d7604da55dMD53123456789/178232018-02-22 23:00:50.301oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-02-23T02:00:50LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
| dc.title.en.fl_str_mv |
Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit |
| title |
Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit |
| spellingShingle |
Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit Nunes-Nesi, Adriano Tomato fruit Ripening Ascorbate Sugars Pyrophosphatase |
| title_short |
Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit |
| title_full |
Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit |
| title_fullStr |
Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit |
| title_full_unstemmed |
Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit |
| title_sort |
Pyrophosphate levels strongly influence ascorbate and starch content in tomato fruit |
| author |
Nunes-Nesi, Adriano |
| author_facet |
Nunes-Nesi, Adriano Osorio, Sonia Stratmann, Marina Fernie, Alisdair R. |
| author_role |
author |
| author2 |
Osorio, Sonia Stratmann, Marina Fernie, Alisdair R. |
| author2_role |
author author author |
| dc.contributor.author.fl_str_mv |
Nunes-Nesi, Adriano Osorio, Sonia Stratmann, Marina Fernie, Alisdair R. |
| dc.subject.pt-BR.fl_str_mv |
Tomato fruit Ripening Ascorbate Sugars Pyrophosphatase |
| topic |
Tomato fruit Ripening Ascorbate Sugars Pyrophosphatase |
| description |
O artigo não contém o resumo em português. |
| publishDate |
2013 |
| dc.date.issued.fl_str_mv |
2013-08-09 |
| dc.date.accessioned.fl_str_mv |
2018-02-22T11:17:35Z |
| dc.date.available.fl_str_mv |
2018-02-22T11:17:35Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://dx.doi.org/10.3389/fpls.2013.00308 http://www.locus.ufv.br/handle/123456789/17823 |
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1664462X |
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1664462X |
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http://dx.doi.org/10.3389/fpls.2013.00308 http://www.locus.ufv.br/handle/123456789/17823 |
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eng |
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v. 4, Article 308, p. 01-13, Agosto 2013 |
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Frontiers in Plant Science |
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Frontiers in Plant Science |
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