Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy

Bibliographic Details
Main Author: Coelho, Polyana Galvão Bernardes
Publication Date: 2018
Other Authors: Souza, Maria Verônica de, Conceição, Lissandro Gonçalves, Viloria, Marlene Isabel Vargas, Bedoya, Sirley Adriana Ortiz
Format: Article
Language: eng
Source: LOCUS Repositório Institucional da UFV
Download full: http://dx.doi.org/10.1590/abd1806-4841.20187544
http://www.locus.ufv.br/handle/123456789/24557
Summary: The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the collagen fibers in the tissues. The authors intend to elucidate all steps to obtain and capture images of histological sections stained with picrosirius red and evaluated under polarized light microscopy, as well as possible artefacts that may occur.
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repository_id_str 2145
spelling Coelho, Polyana Galvão BernardesSouza, Maria Verônica deConceição, Lissandro GonçalvesViloria, Marlene Isabel VargasBedoya, Sirley Adriana Ortiz2019-04-15T14:28:10Z2019-04-15T14:28:10Z2018-051806-4841http://dx.doi.org/10.1590/abd1806-4841.20187544http://www.locus.ufv.br/handle/123456789/24557The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the collagen fibers in the tissues. The authors intend to elucidate all steps to obtain and capture images of histological sections stained with picrosirius red and evaluated under polarized light microscopy, as well as possible artefacts that may occur.engAnais Brasileiros de Dermatologiav. 93, n. 3, p. 415-418, mai.- jun. 2018CollagenMicroscopyPolarizationSkinEvaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf914496https://locus.ufv.br//bitstream/123456789/24557/1/artigo.pdfa65f51c46151b92b0f252a645be6552aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/24557/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/245572019-04-15 11:30:40.786oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-04-15T14:30:40LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
title Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
spellingShingle Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
Coelho, Polyana Galvão Bernardes
Collagen
Microscopy
Polarization
Skin
title_short Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
title_full Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
title_fullStr Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
title_full_unstemmed Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
title_sort Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy
author Coelho, Polyana Galvão Bernardes
author_facet Coelho, Polyana Galvão Bernardes
Souza, Maria Verônica de
Conceição, Lissandro Gonçalves
Viloria, Marlene Isabel Vargas
Bedoya, Sirley Adriana Ortiz
author_role author
author2 Souza, Maria Verônica de
Conceição, Lissandro Gonçalves
Viloria, Marlene Isabel Vargas
Bedoya, Sirley Adriana Ortiz
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Coelho, Polyana Galvão Bernardes
Souza, Maria Verônica de
Conceição, Lissandro Gonçalves
Viloria, Marlene Isabel Vargas
Bedoya, Sirley Adriana Ortiz
dc.subject.pt-BR.fl_str_mv Collagen
Microscopy
Polarization
Skin
topic Collagen
Microscopy
Polarization
Skin
description The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the collagen fibers in the tissues. The authors intend to elucidate all steps to obtain and capture images of histological sections stained with picrosirius red and evaluated under polarized light microscopy, as well as possible artefacts that may occur.
publishDate 2018
dc.date.issued.fl_str_mv 2018-05
dc.date.accessioned.fl_str_mv 2019-04-15T14:28:10Z
dc.date.available.fl_str_mv 2019-04-15T14:28:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/abd1806-4841.20187544
http://www.locus.ufv.br/handle/123456789/24557
dc.identifier.issn.none.fl_str_mv 1806-4841
identifier_str_mv 1806-4841
url http://dx.doi.org/10.1590/abd1806-4841.20187544
http://www.locus.ufv.br/handle/123456789/24557
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 93, n. 3, p. 415-418, mai.- jun. 2018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Anais Brasileiros de Dermatologia
publisher.none.fl_str_mv Anais Brasileiros de Dermatologia
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
bitstream.url.fl_str_mv https://locus.ufv.br//bitstream/123456789/24557/1/artigo.pdf
https://locus.ufv.br//bitstream/123456789/24557/2/license.txt
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repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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