Strychnos pseudoquina and its purified compounds present an effective in vitro antileishmanial activity

• Autor(a) principal: Lage, Paula Sousa
• Data de Publicação: 2013
• Outros Autores: Andrade, Pedro Henrique Rocha de, Lopes, Amanda de Santana, Fumagalli, Miguel Angel Chávez, Valadares, Diogo Garcia, Duarte, Mariana Costa, Lage, Daniela Pagliara, Costa, Lourena Emanuele, Martins, Vivian Tamietti, Ribeiro, Tatiana Gomes, Souza Filho, José Dias de, Tavares, Carlos Alberto Pereira, Pádua, Rodrigo Maia de, Leite, João Paulo Viana, Coelho, Eduardo Antonio Ferraz
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://dx.doi.org/10.1155/2013/304354; http://www.locus.ufv.br/handle/123456789/17021
• topic: Strychnos pseudoquina; In Vitro

The development of new and cost-effective alternative therapeutic strategies to treat leishmaniasis has become a high priority. In the present study, the antileishmanial activity of Strychnos pseudoquina St. Hil. was investigated and pure compounds that presented this biological effect were isolated. An ethyl acetate extract was prepared, and it proved to be effective against Leishmania amazonensis. A bioactivity-guided fractionation was performed, and two flavonoids were identified, quercetin 3-O-methyl ether and strychnobiflavone, which presented an effective antileishmanial activity against L. amazonensis, and studies were extended to establish their minimum inhibitory concentrations (IC50), their leishmanicidal effects on the intra-macrophage Leishmania stage, as well as their cytotoxic effects on murine macrophages (CC50), and in O+ human red blood cells. The data presented in this study showed the potential of an ethyl acetate extract of S. pseudoquina, as well as two flavonoids purified from it, which can be used as a therapeutic alternative on its own, or in association with other drugs, to treat disease evoked by L. amazonensis.