The influence of alternative pathways of respiration that utilize branched‐chain amino acids following water shortage in Arabidopsis
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Publication Date: | 2015 |
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Format: | Article |
Language: | eng |
Source: | LOCUS Repositório Institucional da UFV |
Download full: | https://doi.org/10.1111/pce.12682 http://www.locus.ufv.br/handle/123456789/19655 |
Summary: | During dark‐induced senescence isovaleryl‐CoA dehydrogenase (IVDH) and D‐2‐hydroxyglutarate dehydrogenase (D‐2HGDH) act as alternate electron donors to the ubiquinol pool via the electron‐transfer flavoprotein/electron‐transfer flavoprotein:ubiquinone oxidoreductase (ETF/ETFQO) pathway. However, the role of this pathway in response to other stresses still remains unclear. Here, we demonstrated that this alternative pathway is associated with tolerance to drought in Arabidopsis. In comparison with wild type (WT) and lines overexpressing D‐2GHDH, loss‐of‐function etfqo‐1, d2hgdh‐2 and ivdh‐1 mutants displayed compromised respiration rates and were more sensitive to drought. Our results demonstrated that an operational ETF/ETFQO pathway is associated with plants' ability to withstand drought and to recover growth once water becomes replete. Drought‐induced metabolic reprogramming resulted in an increase in tricarboxylic acid (TCA) cycle intermediates and total amino acid levels, as well as decreases in protein, starch and nitrate contents. The enhanced levels of the branched‐chain amino acids in loss‐of‐function mutants appear to be related to their increased utilization as substrates for the TCA cycle under water stress. Our results thus show that mitochondrial metabolism is highly active during drought stress responses and provide support for a role of alternative respiratory pathways within this response. |
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Pires, Marcel V.Pereira Júnior, Adilson A.Medeiros, David B.Daloso, Danilo M.Pham, Phuong AnhBarros, Kallyne A.Engqvist, Martin K. M.Florian, AlexandraKrahnert, InaMaurino, Veronica G.Araújo, Wagner L.Fernie, Alisdair R.2018-05-17T14:54:13Z2018-05-17T14:54:13Z2015-11-3013653040https://doi.org/10.1111/pce.12682http://www.locus.ufv.br/handle/123456789/19655During dark‐induced senescence isovaleryl‐CoA dehydrogenase (IVDH) and D‐2‐hydroxyglutarate dehydrogenase (D‐2HGDH) act as alternate electron donors to the ubiquinol pool via the electron‐transfer flavoprotein/electron‐transfer flavoprotein:ubiquinone oxidoreductase (ETF/ETFQO) pathway. However, the role of this pathway in response to other stresses still remains unclear. Here, we demonstrated that this alternative pathway is associated with tolerance to drought in Arabidopsis. In comparison with wild type (WT) and lines overexpressing D‐2GHDH, loss‐of‐function etfqo‐1, d2hgdh‐2 and ivdh‐1 mutants displayed compromised respiration rates and were more sensitive to drought. Our results demonstrated that an operational ETF/ETFQO pathway is associated with plants' ability to withstand drought and to recover growth once water becomes replete. Drought‐induced metabolic reprogramming resulted in an increase in tricarboxylic acid (TCA) cycle intermediates and total amino acid levels, as well as decreases in protein, starch and nitrate contents. The enhanced levels of the branched‐chain amino acids in loss‐of‐function mutants appear to be related to their increased utilization as substrates for the TCA cycle under water stress. Our results thus show that mitochondrial metabolism is highly active during drought stress responses and provide support for a role of alternative respiratory pathways within this response.engPlant, Cell and Environmentv. 39, Issue 6, p. 1304-1319, June 2016Branched-chain amino acidsDroughtETF/ ETFQO pathwayMetabolomicsRespirationTricarboxylic acid cycleThe influence of alternative pathways of respiration that utilize branched‐chain amino acids following water shortage in Arabidopsisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf1820700https://locus.ufv.br//bitstream/123456789/19655/1/artigo.pdfc64fc02fba9392b81401e08dbbd0943cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/19655/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg6869https://locus.ufv.br//bitstream/123456789/19655/3/artigo.pdf.jpgcb6b3fced3090662f7e72afd6a1cc66aMD53123456789/196552018-05-17 23:00:45.177oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-05-18T02:00:45LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
The influence of alternative pathways of respiration that utilize branched‐chain amino acids following water shortage in Arabidopsis |
title |
The influence of alternative pathways of respiration that utilize branched‐chain amino acids following water shortage in Arabidopsis |
spellingShingle |
The influence of alternative pathways of respiration that utilize branched‐chain amino acids following water shortage in Arabidopsis Pires, Marcel V. Branched-chain amino acids Drought ETF/ ETFQO pathway Metabolomics Respiration Tricarboxylic acid cycle |
title_short |
The influence of alternative pathways of respiration that utilize branched‐chain amino acids following water shortage in Arabidopsis |
title_full |
The influence of alternative pathways of respiration that utilize branched‐chain amino acids following water shortage in Arabidopsis |
title_fullStr |
The influence of alternative pathways of respiration that utilize branched‐chain amino acids following water shortage in Arabidopsis |
title_full_unstemmed |
The influence of alternative pathways of respiration that utilize branched‐chain amino acids following water shortage in Arabidopsis |
title_sort |
The influence of alternative pathways of respiration that utilize branched‐chain amino acids following water shortage in Arabidopsis |
author |
Pires, Marcel V. |
author_facet |
Pires, Marcel V. Pereira Júnior, Adilson A. Medeiros, David B. Daloso, Danilo M. Pham, Phuong Anh Barros, Kallyne A. Engqvist, Martin K. M. Florian, Alexandra Krahnert, Ina Maurino, Veronica G. Araújo, Wagner L. Fernie, Alisdair R. |
author_role |
author |
author2 |
Pereira Júnior, Adilson A. Medeiros, David B. Daloso, Danilo M. Pham, Phuong Anh Barros, Kallyne A. Engqvist, Martin K. M. Florian, Alexandra Krahnert, Ina Maurino, Veronica G. Araújo, Wagner L. Fernie, Alisdair R. |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pires, Marcel V. Pereira Júnior, Adilson A. Medeiros, David B. Daloso, Danilo M. Pham, Phuong Anh Barros, Kallyne A. Engqvist, Martin K. M. Florian, Alexandra Krahnert, Ina Maurino, Veronica G. Araújo, Wagner L. Fernie, Alisdair R. |
dc.subject.pt-BR.fl_str_mv |
Branched-chain amino acids Drought ETF/ ETFQO pathway Metabolomics Respiration Tricarboxylic acid cycle |
topic |
Branched-chain amino acids Drought ETF/ ETFQO pathway Metabolomics Respiration Tricarboxylic acid cycle |
description |
During dark‐induced senescence isovaleryl‐CoA dehydrogenase (IVDH) and D‐2‐hydroxyglutarate dehydrogenase (D‐2HGDH) act as alternate electron donors to the ubiquinol pool via the electron‐transfer flavoprotein/electron‐transfer flavoprotein:ubiquinone oxidoreductase (ETF/ETFQO) pathway. However, the role of this pathway in response to other stresses still remains unclear. Here, we demonstrated that this alternative pathway is associated with tolerance to drought in Arabidopsis. In comparison with wild type (WT) and lines overexpressing D‐2GHDH, loss‐of‐function etfqo‐1, d2hgdh‐2 and ivdh‐1 mutants displayed compromised respiration rates and were more sensitive to drought. Our results demonstrated that an operational ETF/ETFQO pathway is associated with plants' ability to withstand drought and to recover growth once water becomes replete. Drought‐induced metabolic reprogramming resulted in an increase in tricarboxylic acid (TCA) cycle intermediates and total amino acid levels, as well as decreases in protein, starch and nitrate contents. The enhanced levels of the branched‐chain amino acids in loss‐of‐function mutants appear to be related to their increased utilization as substrates for the TCA cycle under water stress. Our results thus show that mitochondrial metabolism is highly active during drought stress responses and provide support for a role of alternative respiratory pathways within this response. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-11-30 |
dc.date.accessioned.fl_str_mv |
2018-05-17T14:54:13Z |
dc.date.available.fl_str_mv |
2018-05-17T14:54:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1111/pce.12682 http://www.locus.ufv.br/handle/123456789/19655 |
dc.identifier.issn.none.fl_str_mv |
13653040 |
identifier_str_mv |
13653040 |
url |
https://doi.org/10.1111/pce.12682 http://www.locus.ufv.br/handle/123456789/19655 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 39, Issue 6, p. 1304-1319, June 2016 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Plant, Cell and Environment |
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Plant, Cell and Environment |
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