A RAPD marker associated with B chromosomes in Partamona helleri (Hymenoptera, Apidae)

• Autor(a) principal: Tosta, V.C.
• Data de Publicação: 2004
• Outros Autores: Fernandes-Salomão, T.M., Tavares, M.G., Pompolo, S.G., Barros, E.G., Campos, L.A.O.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: https://doi.org/10.1159/000079299; http://www.locus.ufv.br/handle/123456789/19126
• topic: RAPD marker; B chromosomes; Partamona helleri

The hymenopteran Partamona helleri is found in southwestern Brazil in the Mata Atlântica from the north of the state of Santa Catarina until the south of Bahia. This work shows that P. helleri can carry up to four B chromosomes per individual. In order to obtain more information about P. helleri B chromosomes, the RAPD technique was used to detect DNA fragments associated with these chromosomes. The results showed that the RAPD technique is useful to detect specific sequences associated with B chromosomes. One RAPD marker was identified, cloned and used as probe in a DNA blot analysis. This RAPD marker hybridized with sequences present only in individuals containing B chromosomes.