Determinantes virais associados às diferentes propriedades biológicas de dois isolados de Lettuce mosaic virus (LMV): cinética da infecção viral e infecção dos tecidos embrionários

Detalhes bibliográficos
Autor(a) principal: Beserra Júnior, José Evando Aguiar
Data de Publicação: 2008
Tipo de documento: Tese
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/1020
Resumo: Lettuce mosaic, caused by the potyvirus Lettuce mosaic virus (LMV), is one of the main diseases of lettuce. The type strain of the virus includes isolates which are seed borne in lettuce, but which cannot infect lettuce cultivars harboring the recessive resistance aleles mo11 e mo12. The Most strain includes isolates which are also seed borne and can infect cultivars with the recessive resistance genes. The Brazilian isolate AF199 belongs to the Most strain. Its seed transmission rate can be as high as 16,5%. This isolate induces severe symptoms in Nicotiana benthamiana and has a shorter latent period in comparison to isolate E , collected in Spain. Furthermore, this isolate induces systemic necrosis in some lettuce cultivars. Isolate E can infect cultivars with the recessive resistance genes, but it is not seed borne in these cultivars. The objectives of this work were: (i) to analyze the tissues of ovules and embryos from lettuce plants infected by isolates AF199 and E; (ii) to study the fitness mechanisms of isolates AF199 and E, and of three recombinants obtained between these two isolates, in lettuce Salinas 88 and N. benthamiana. Isolate AF199 was detected in all tissues of ovules collected before and after fertilization. Conversely, isolate E was not detected in any ovule tissues, before or after fertilization. These results suggest that the absence of seed transmission of isolate E is due to the inability of viral particles in reaching the reproductive tissues, and that viral factors related to movement within the plant could be involved in seed transmission. The kinetics of systemic infection was analyzed in plants in which the inoculated leaf was removed after different periods of time. Viral RNA accumulation was analyzed in inoculated and non-inoculated leaves. The results indicate that isolate AF199 reaches a higher concentration in both inoculated and non-inoculated leaves, earlier than isolate E, in both hosts. The recombinant Rec1 induced distinct symptoms and reached a higher titer in N. benthamiana compared to the parental isolates. Rec4 reached the lowest concentration among the isolates/recombinants tested in N. benthamiana. In lettuce, the relative concentrations of the recombinants Rec3 and Rec4 was up to 10-fold higher in comparison to the AF199 isolate. It is concluded that isolate AF199, and those recombinants having the P1/HC-Pro coding region derived from this isolate, are better adapted than isolates having the P1-HC/Pro region derived from isolate E. This better adaptability could be related to the suppression of RNA silencing induced by P1/HC-Pro.
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spelling Beserra Júnior, José Evando Aguiarhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4762174A2Carvalho, Murilo Geraldo dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787250U5Krause-sakate, Renatehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763879Y2Zerbini Júnior, Francisco Murilohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5Silva, Maitê Vaslin de Freitashttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785843E7Zerbini, Poliane Alfenashttp://lattes.cnpq.br/8632328159533071Paula, Sérgio Oliveira dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767540P42015-03-26T12:41:51Z2008-07-222015-03-26T12:41:51Z2008-05-27BESERRA JÚNIOR, José Evando Aguiar. Viral determinants associated with the distinct biological properties of two isolates of Lettuce mosaic virus (LMV): Kinetics of viral infection and infection of the embrionary tissues. 2008. 89 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2008.http://locus.ufv.br/handle/123456789/1020Lettuce mosaic, caused by the potyvirus Lettuce mosaic virus (LMV), is one of the main diseases of lettuce. The type strain of the virus includes isolates which are seed borne in lettuce, but which cannot infect lettuce cultivars harboring the recessive resistance aleles mo11 e mo12. The Most strain includes isolates which are also seed borne and can infect cultivars with the recessive resistance genes. The Brazilian isolate AF199 belongs to the Most strain. Its seed transmission rate can be as high as 16,5%. This isolate induces severe symptoms in Nicotiana benthamiana and has a shorter latent period in comparison to isolate E , collected in Spain. Furthermore, this isolate induces systemic necrosis in some lettuce cultivars. Isolate E can infect cultivars with the recessive resistance genes, but it is not seed borne in these cultivars. The objectives of this work were: (i) to analyze the tissues of ovules and embryos from lettuce plants infected by isolates AF199 and E; (ii) to study the fitness mechanisms of isolates AF199 and E, and of three recombinants obtained between these two isolates, in lettuce Salinas 88 and N. benthamiana. Isolate AF199 was detected in all tissues of ovules collected before and after fertilization. Conversely, isolate E was not detected in any ovule tissues, before or after fertilization. These results suggest that the absence of seed transmission of isolate E is due to the inability of viral particles in reaching the reproductive tissues, and that viral factors related to movement within the plant could be involved in seed transmission. The kinetics of systemic infection was analyzed in plants in which the inoculated leaf was removed after different periods of time. Viral RNA accumulation was analyzed in inoculated and non-inoculated leaves. The results indicate that isolate AF199 reaches a higher concentration in both inoculated and non-inoculated leaves, earlier than isolate E, in both hosts. The recombinant Rec1 induced distinct symptoms and reached a higher titer in N. benthamiana compared to the parental isolates. Rec4 reached the lowest concentration among the isolates/recombinants tested in N. benthamiana. In lettuce, the relative concentrations of the recombinants Rec3 and Rec4 was up to 10-fold higher in comparison to the AF199 isolate. It is concluded that isolate AF199, and those recombinants having the P1/HC-Pro coding region derived from this isolate, are better adapted than isolates having the P1-HC/Pro region derived from isolate E. This better adaptability could be related to the suppression of RNA silencing induced by P1/HC-Pro.O mosaico da alface, causado pelo potyvírus Lettuce mosaic virus (LMV), é uma das principais doenças da cultura. A estirpe típica do vírus inclui isolados transmitidos pela semente, mas que não infectam cultivares de alface contendo os alelos de resistência recessivos mo11 e mo12. A estirpe Most inclui isolados transmitidos pela semente e que infectam tais cultivares. O isolado brasileiro AF199 pertence à estirpe Most. A taxa de transmissão pela semente desse isolado chega a 16,5%. Esse isolado induz sintomas mais severos em Nicotiana benthamiana e possui período latente mais curto em comparação ao isolado E , coletado na Espanha. Além disso, esse isolado é capaz de induzir necrose sistêmica em certas cultivares de alface. O isolado E infecta cultivares contendo os genes de resistência recessivos, mas não é transmitido pela semente nessas cultivares. O presente trabalho teve como objetivos: (i) analisar os tecidos de óvulos e embriões de alface infectados pelos isolados AF199 e E; (ii) estudar os mecanismos de adaptabilidade dos isolados AF199 e E, e de três recombinantes obtidos entre eles, na cultivar de alface Salinas 88 e em N. benthamiana. O isolado AF199 foi detectado em todos os tecidos de óvulos coletados antes ou após a fertilização. Já o isolado E não foi detectado em nenhum dos tecidos do óvulo, antes ou após a fertilização. Esses resultados indicam que a não transmissão pela semente do isolado E se deve ao fato das partículas virais não serem capazes de atingir o tecido reprodutivo feminino, e sugerem que fatores relacionados ao movimento do vírus na planta podem estar envolvidos no processo de transmissão do LMV pela semente. A cinética de infecção viral foi analisada em plantas nas quais a folha inoculada foi destacada após diferentes períodos de tempo. O acúmulo de RNA viral foi analisado em folhas inoculadas e não-inoculadas. Os resultados indicam que o isolado AF199 atinge maior concentração nas folhas inoculada e não-inoculada mais cedo que o isolado E, em ambos os hospedeiros. O recombinante Rec1 induziu sintomas distintos dos parentais e acumulou mais em N. benthamiana. O recombinante Rec4 apresentou menor acúmulo dentre todos os isolados/recombinantes testados em N. benthamiana. Em alface, os recombinantes Rec3 e Rec4 apresentaram acúmulo cerca de 10 vezes superior ao isolado AF199. Conclui-se que o isolado AF199, e os recombinantes que possuem a região P1/HC-Pro desse isolado, são mais adaptados do que isolados que possuem essa região do isolado E. Essa melhor adaptação poderia estar relacionada à supressão do silenciamento gênico induzida por P1/HC-Pro.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaDoutorado em FitopatologiaUFVBREtiologia; Epidemiologia; ControleLettuce mosaic virusPotyvirusAlfaceResistênciaLettuce mosaic virusPotyvirusLettuceResistanceCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIADeterminantes virais associados às diferentes propriedades biológicas de dois isolados de Lettuce mosaic virus (LMV): cinética da infecção viral e infecção dos tecidos embrionáriosViral determinants associated with the distinct biological properties of two isolates of Lettuce mosaic virus (LMV): Kinetics of viral infection and infection of the embrionary tissuesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf905646https://locus.ufv.br//bitstream/123456789/1020/1/texto%20completo.pdf83b810ad4d298ca7fd427a16b6bfa011MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain156421https://locus.ufv.br//bitstream/123456789/1020/2/texto%20completo.pdf.txt64f675c7da1826d544be265dca384eb8MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3658https://locus.ufv.br//bitstream/123456789/1020/3/texto%20completo.pdf.jpgf1f47e81632b1b70be0de988bf121e75MD53123456789/10202016-04-06 23:16:34.946oai:locus.ufv.br:123456789/1020Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-07T02:16:34LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Determinantes virais associados às diferentes propriedades biológicas de dois isolados de Lettuce mosaic virus (LMV): cinética da infecção viral e infecção dos tecidos embrionários
dc.title.alternative.eng.fl_str_mv Viral determinants associated with the distinct biological properties of two isolates of Lettuce mosaic virus (LMV): Kinetics of viral infection and infection of the embrionary tissues
title Determinantes virais associados às diferentes propriedades biológicas de dois isolados de Lettuce mosaic virus (LMV): cinética da infecção viral e infecção dos tecidos embrionários
spellingShingle Determinantes virais associados às diferentes propriedades biológicas de dois isolados de Lettuce mosaic virus (LMV): cinética da infecção viral e infecção dos tecidos embrionários
Beserra Júnior, José Evando Aguiar
Lettuce mosaic virus
Potyvirus
Alface
Resistência
Lettuce mosaic virus
Potyvirus
Lettuce
Resistance
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
title_short Determinantes virais associados às diferentes propriedades biológicas de dois isolados de Lettuce mosaic virus (LMV): cinética da infecção viral e infecção dos tecidos embrionários
title_full Determinantes virais associados às diferentes propriedades biológicas de dois isolados de Lettuce mosaic virus (LMV): cinética da infecção viral e infecção dos tecidos embrionários
title_fullStr Determinantes virais associados às diferentes propriedades biológicas de dois isolados de Lettuce mosaic virus (LMV): cinética da infecção viral e infecção dos tecidos embrionários
title_full_unstemmed Determinantes virais associados às diferentes propriedades biológicas de dois isolados de Lettuce mosaic virus (LMV): cinética da infecção viral e infecção dos tecidos embrionários
title_sort Determinantes virais associados às diferentes propriedades biológicas de dois isolados de Lettuce mosaic virus (LMV): cinética da infecção viral e infecção dos tecidos embrionários
author Beserra Júnior, José Evando Aguiar
author_facet Beserra Júnior, José Evando Aguiar
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4762174A2
dc.contributor.author.fl_str_mv Beserra Júnior, José Evando Aguiar
dc.contributor.advisor-co1.fl_str_mv Carvalho, Murilo Geraldo de
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787250U5
dc.contributor.advisor-co2.fl_str_mv Krause-sakate, Renate
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763879Y2
dc.contributor.advisor1.fl_str_mv Zerbini Júnior, Francisco Murilo
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5
dc.contributor.referee1.fl_str_mv Silva, Maitê Vaslin de Freitas
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785843E7
dc.contributor.referee2.fl_str_mv Zerbini, Poliane Alfenas
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/8632328159533071
dc.contributor.referee3.fl_str_mv Paula, Sérgio Oliveira de
dc.contributor.referee3Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767540P4
contributor_str_mv Carvalho, Murilo Geraldo de
Krause-sakate, Renate
Zerbini Júnior, Francisco Murilo
Silva, Maitê Vaslin de Freitas
Zerbini, Poliane Alfenas
Paula, Sérgio Oliveira de
dc.subject.por.fl_str_mv Lettuce mosaic virus
Potyvirus
Alface
Resistência
topic Lettuce mosaic virus
Potyvirus
Alface
Resistência
Lettuce mosaic virus
Potyvirus
Lettuce
Resistance
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
dc.subject.eng.fl_str_mv Lettuce mosaic virus
Potyvirus
Lettuce
Resistance
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
description Lettuce mosaic, caused by the potyvirus Lettuce mosaic virus (LMV), is one of the main diseases of lettuce. The type strain of the virus includes isolates which are seed borne in lettuce, but which cannot infect lettuce cultivars harboring the recessive resistance aleles mo11 e mo12. The Most strain includes isolates which are also seed borne and can infect cultivars with the recessive resistance genes. The Brazilian isolate AF199 belongs to the Most strain. Its seed transmission rate can be as high as 16,5%. This isolate induces severe symptoms in Nicotiana benthamiana and has a shorter latent period in comparison to isolate E , collected in Spain. Furthermore, this isolate induces systemic necrosis in some lettuce cultivars. Isolate E can infect cultivars with the recessive resistance genes, but it is not seed borne in these cultivars. The objectives of this work were: (i) to analyze the tissues of ovules and embryos from lettuce plants infected by isolates AF199 and E; (ii) to study the fitness mechanisms of isolates AF199 and E, and of three recombinants obtained between these two isolates, in lettuce Salinas 88 and N. benthamiana. Isolate AF199 was detected in all tissues of ovules collected before and after fertilization. Conversely, isolate E was not detected in any ovule tissues, before or after fertilization. These results suggest that the absence of seed transmission of isolate E is due to the inability of viral particles in reaching the reproductive tissues, and that viral factors related to movement within the plant could be involved in seed transmission. The kinetics of systemic infection was analyzed in plants in which the inoculated leaf was removed after different periods of time. Viral RNA accumulation was analyzed in inoculated and non-inoculated leaves. The results indicate that isolate AF199 reaches a higher concentration in both inoculated and non-inoculated leaves, earlier than isolate E, in both hosts. The recombinant Rec1 induced distinct symptoms and reached a higher titer in N. benthamiana compared to the parental isolates. Rec4 reached the lowest concentration among the isolates/recombinants tested in N. benthamiana. In lettuce, the relative concentrations of the recombinants Rec3 and Rec4 was up to 10-fold higher in comparison to the AF199 isolate. It is concluded that isolate AF199, and those recombinants having the P1/HC-Pro coding region derived from this isolate, are better adapted than isolates having the P1-HC/Pro region derived from isolate E. This better adaptability could be related to the suppression of RNA silencing induced by P1/HC-Pro.
publishDate 2008
dc.date.available.fl_str_mv 2008-07-22
2015-03-26T12:41:51Z
dc.date.issued.fl_str_mv 2008-05-27
dc.date.accessioned.fl_str_mv 2015-03-26T12:41:51Z
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dc.identifier.citation.fl_str_mv BESERRA JÚNIOR, José Evando Aguiar. Viral determinants associated with the distinct biological properties of two isolates of Lettuce mosaic virus (LMV): Kinetics of viral infection and infection of the embrionary tissues. 2008. 89 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2008.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/1020
identifier_str_mv BESERRA JÚNIOR, José Evando Aguiar. Viral determinants associated with the distinct biological properties of two isolates of Lettuce mosaic virus (LMV): Kinetics of viral infection and infection of the embrionary tissues. 2008. 89 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2008.
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dc.publisher.department.fl_str_mv Etiologia; Epidemiologia; Controle
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