Estrutura e ultra-estrutura dos espermatozoides de Diaphorina citri (Hemiptera, Sternorryncha, Liviidae)
Main Author: | |
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Publication Date: | 2014 |
Format: | Master thesis |
Language: | por |
Source: | LOCUS Repositório Institucional da UFV |
Download full: | http://locus.ufv.br/handle/123456789/2374 |
Summary: | The Hemiptera comprises a diverse group of insects with approximately 89,000 described species and distributed throughout the world. Currently, four suborders of Hemiptera are recognized: Heteroptera, Sternorryncha, Coleorryncha and Auchenorryncha. The suborder Sternorryncha form a monophyletic group with approximately 16,000 described species, distributed in four superfamilies: Aleyrodoidea, Aphidoidea, Coccoidea and Psyloidea. Some species of this suborder are among the major pests of agriculture worldwide. One example is Diaphorina citri Kuwayama (Liviidae), popularly known as the leafhopper, which today is one of the most important and devastating citrus pest worldwide. Considering knowledge about the reproductive biology as well as systematic may contribute to the management of pest, in this paper we describe the structure and ultrastructure of spermatozoa of D. citri. For this, were used light, transmission and scanning electronic microscopy. In this species we observed that the sperm measure about 525 μm in length, are free in the seminal vesicle and do not show polymorphism. As observed in other Psyloidea, the sperm when manipulated opens into two filaments, one in which its anterior end is attached to the nuclei and the other free. Through the flagellum there are lateral projections with about 2 μm in length, this characteristic is reported for another Psyloidea and Aleyrodoidea. Furthermore, at the end of the flagellum three appendages are observed with approximately 7 μm, which were observed so far only in D. citri. The head region is formed by nuclei with compact chromatin and a structure comprising by a matherial with median electron density and it extends extends about 25 μm in length in front of the nuclei. The flagellum consists of an axoneme (9 + 9 + 2), two mitochondrial derivatives and two accessory bodies. The presence of lateral projections is a characteristic shared by Psyloidea and Aleirodoidea, already the sperm opening in two filaments when manipulated occurs only in Psyloidea, and the presence of the three appendices at the end of the flagellum distinguishes D. citri from the other Psyloidea species studied. |
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Barcellos, Marcelo Silvahttp://lattes.cnpq.br/8583347449567713Lino Neto, Joséhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786229P1Araújo, Vinícius Albanohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4772784T8Oliveira, Cláudia Vânia Miranda dehttp://lattes.cnpq.br/44864628455347462015-03-26T13:04:20Z2015-01-262015-03-26T13:04:20Z2014-02-19BARCELLOS, Marcelo Silva. Structure and ultrastructure of Diaphorina citri spermatozoa (Hemiptera, Sternorryncha, Liviidae). 2014. 29 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2014.http://locus.ufv.br/handle/123456789/2374The Hemiptera comprises a diverse group of insects with approximately 89,000 described species and distributed throughout the world. Currently, four suborders of Hemiptera are recognized: Heteroptera, Sternorryncha, Coleorryncha and Auchenorryncha. The suborder Sternorryncha form a monophyletic group with approximately 16,000 described species, distributed in four superfamilies: Aleyrodoidea, Aphidoidea, Coccoidea and Psyloidea. Some species of this suborder are among the major pests of agriculture worldwide. One example is Diaphorina citri Kuwayama (Liviidae), popularly known as the leafhopper, which today is one of the most important and devastating citrus pest worldwide. Considering knowledge about the reproductive biology as well as systematic may contribute to the management of pest, in this paper we describe the structure and ultrastructure of spermatozoa of D. citri. For this, were used light, transmission and scanning electronic microscopy. In this species we observed that the sperm measure about 525 μm in length, are free in the seminal vesicle and do not show polymorphism. As observed in other Psyloidea, the sperm when manipulated opens into two filaments, one in which its anterior end is attached to the nuclei and the other free. Through the flagellum there are lateral projections with about 2 μm in length, this characteristic is reported for another Psyloidea and Aleyrodoidea. Furthermore, at the end of the flagellum three appendages are observed with approximately 7 μm, which were observed so far only in D. citri. The head region is formed by nuclei with compact chromatin and a structure comprising by a matherial with median electron density and it extends extends about 25 μm in length in front of the nuclei. The flagellum consists of an axoneme (9 + 9 + 2), two mitochondrial derivatives and two accessory bodies. The presence of lateral projections is a characteristic shared by Psyloidea and Aleirodoidea, already the sperm opening in two filaments when manipulated occurs only in Psyloidea, and the presence of the three appendices at the end of the flagellum distinguishes D. citri from the other Psyloidea species studied.Atualmente quatro subordens de Hemiptera são reconhecidas: Heteroptera, Sternorryncha, Coleorryncha e Auchenorryncha. A subordem Sternorryncha forma um grupo monofilético com aproximadamente 16 mil espécies descritas e distribuídas em quatro superfamílias: Aleyrodoidea, Aphidoidea, Coccoidea e Psylloidea. Algumas espécies dessa subordem estão entre as principais pragas da agricultura mundial. Um exemplo é a Diaphorina citri Kuwayama (Liviidae), popularmente conhecida como cigarrinha- do-limão, que hoje é uma das mais importantes e devastadoras pragas da citricultura mundial. Considerando que conhecer a biologia reprodutiva, bem como a sistemática, pode contribuir para o manejo de espécies-praga, neste trabalho descrevemos a estrutura e ultra-estrutura dos espermatozóides de D. citri. Para isso foram utilizadas microscopias de luz e eletrônicas de transmissão e varredura. Nesta espécie observamos que os espermatozoides medem cerca de 525 μm de comprimento, estão livres na vesícula seminal e não apresentam polimorfismo. Como observado em outros Psylloidea, o espermatozoide, quando manipulado, se abre em dois filamentos, onde um deles em sua extremidade anterior está preso ao núcleo e o outro livre. Ao longo de todo o flagelo existem projeções laterais com cerca de 2 μm de comprimento, característica descrita para outros Psylloidea e, também, Aleyrodoidea. Ainda, na extremidade final da cauda são observadas três apêndices de aproximadamente 7 μm, os quais foram observados até o momento apenas em D. citri. A região da cabeça é formada pelo núcleo com cromatina bastante compacta e por uma estrutura composta de material de densidade eletrônica mediana que se estende cerca de 25 μm de comprimento à frente do núcleo. O flagelo é constituído pelo axonema (9 + 9 + 2), dois derivados mitocondriais e dois corpos acessórios. A presença de projeções laterais é uma característica compartilhada por Psylloidea e Aleirodoidea, já os espermatozoides se abrindo em dois filamentos quando manipulados ocorre apenas em Psylloidea e, a presença dos três apêndices no final do flagelo, distingue D. citri das demais espécies de Psylloidea estudadas.Fundação de Amparo a Pesquisa do Estado de Minas Geraisapplication/pdfporUniversidade Federal de ViçosaMestrado em Biologia Celular e EstruturalUFVBRAnálises quantitativas e moleculares do Genoma; Biologia das células e dos tecidosDiaphorina citriCigarra - EspermatozoidesCigarra- Doenças e pragasDiaphorina citriCicada - SpermCicada - Diseases and pestsCNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERALEstrutura e ultra-estrutura dos espermatozoides de Diaphorina citri (Hemiptera, Sternorryncha, Liviidae)Structure and ultrastructure of Diaphorina citri spermatozoa (Hemiptera, Sternorryncha, Liviidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf385304https://locus.ufv.br//bitstream/123456789/2374/1/texto%20completo.pdf1e6fbd00f08825bd3b21e66ec25f3bd4MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain54400https://locus.ufv.br//bitstream/123456789/2374/2/texto%20completo.pdf.txt3b80d853600ddecf990f17b0d04ee401MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3633https://locus.ufv.br//bitstream/123456789/2374/3/texto%20completo.pdf.jpg58702e59eed6bab5e1adafe422ee2b58MD53123456789/23742016-04-08 23:06:03.62oai:locus.ufv.br:123456789/2374Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-09T02:06:03LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Estrutura e ultra-estrutura dos espermatozoides de Diaphorina citri (Hemiptera, Sternorryncha, Liviidae) |
dc.title.alternative.eng.fl_str_mv |
Structure and ultrastructure of Diaphorina citri spermatozoa (Hemiptera, Sternorryncha, Liviidae) |
title |
Estrutura e ultra-estrutura dos espermatozoides de Diaphorina citri (Hemiptera, Sternorryncha, Liviidae) |
spellingShingle |
Estrutura e ultra-estrutura dos espermatozoides de Diaphorina citri (Hemiptera, Sternorryncha, Liviidae) Barcellos, Marcelo Silva Diaphorina citri Cigarra - Espermatozoides Cigarra- Doenças e pragas Diaphorina citri Cicada - Sperm Cicada - Diseases and pests CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Estrutura e ultra-estrutura dos espermatozoides de Diaphorina citri (Hemiptera, Sternorryncha, Liviidae) |
title_full |
Estrutura e ultra-estrutura dos espermatozoides de Diaphorina citri (Hemiptera, Sternorryncha, Liviidae) |
title_fullStr |
Estrutura e ultra-estrutura dos espermatozoides de Diaphorina citri (Hemiptera, Sternorryncha, Liviidae) |
title_full_unstemmed |
Estrutura e ultra-estrutura dos espermatozoides de Diaphorina citri (Hemiptera, Sternorryncha, Liviidae) |
title_sort |
Estrutura e ultra-estrutura dos espermatozoides de Diaphorina citri (Hemiptera, Sternorryncha, Liviidae) |
author |
Barcellos, Marcelo Silva |
author_facet |
Barcellos, Marcelo Silva |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/8583347449567713 |
dc.contributor.author.fl_str_mv |
Barcellos, Marcelo Silva |
dc.contributor.advisor1.fl_str_mv |
Lino Neto, José |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786229P1 |
dc.contributor.referee1.fl_str_mv |
Araújo, Vinícius Albano |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4772784T8 |
dc.contributor.referee2.fl_str_mv |
Oliveira, Cláudia Vânia Miranda de |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/4486462845534746 |
contributor_str_mv |
Lino Neto, José Araújo, Vinícius Albano Oliveira, Cláudia Vânia Miranda de |
dc.subject.por.fl_str_mv |
Diaphorina citri Cigarra - Espermatozoides Cigarra- Doenças e pragas |
topic |
Diaphorina citri Cigarra - Espermatozoides Cigarra- Doenças e pragas Diaphorina citri Cicada - Sperm Cicada - Diseases and pests CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Diaphorina citri Cicada - Sperm Cicada - Diseases and pests |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
The Hemiptera comprises a diverse group of insects with approximately 89,000 described species and distributed throughout the world. Currently, four suborders of Hemiptera are recognized: Heteroptera, Sternorryncha, Coleorryncha and Auchenorryncha. The suborder Sternorryncha form a monophyletic group with approximately 16,000 described species, distributed in four superfamilies: Aleyrodoidea, Aphidoidea, Coccoidea and Psyloidea. Some species of this suborder are among the major pests of agriculture worldwide. One example is Diaphorina citri Kuwayama (Liviidae), popularly known as the leafhopper, which today is one of the most important and devastating citrus pest worldwide. Considering knowledge about the reproductive biology as well as systematic may contribute to the management of pest, in this paper we describe the structure and ultrastructure of spermatozoa of D. citri. For this, were used light, transmission and scanning electronic microscopy. In this species we observed that the sperm measure about 525 μm in length, are free in the seminal vesicle and do not show polymorphism. As observed in other Psyloidea, the sperm when manipulated opens into two filaments, one in which its anterior end is attached to the nuclei and the other free. Through the flagellum there are lateral projections with about 2 μm in length, this characteristic is reported for another Psyloidea and Aleyrodoidea. Furthermore, at the end of the flagellum three appendages are observed with approximately 7 μm, which were observed so far only in D. citri. The head region is formed by nuclei with compact chromatin and a structure comprising by a matherial with median electron density and it extends extends about 25 μm in length in front of the nuclei. The flagellum consists of an axoneme (9 + 9 + 2), two mitochondrial derivatives and two accessory bodies. The presence of lateral projections is a characteristic shared by Psyloidea and Aleirodoidea, already the sperm opening in two filaments when manipulated occurs only in Psyloidea, and the presence of the three appendices at the end of the flagellum distinguishes D. citri from the other Psyloidea species studied. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-02-19 |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:04:20Z |
dc.date.available.fl_str_mv |
2015-01-26 2015-03-26T13:04:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BARCELLOS, Marcelo Silva. Structure and ultrastructure of Diaphorina citri spermatozoa (Hemiptera, Sternorryncha, Liviidae). 2014. 29 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2014. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/2374 |
identifier_str_mv |
BARCELLOS, Marcelo Silva. Structure and ultrastructure of Diaphorina citri spermatozoa (Hemiptera, Sternorryncha, Liviidae). 2014. 29 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2014. |
url |
http://locus.ufv.br/handle/123456789/2374 |
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por |
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Universidade Federal de Viçosa |
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Mestrado em Biologia Celular e Estrutural |
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UFV |
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BR |
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Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos |
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Universidade Federal de Viçosa |
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