Histological study of micrografting in passionfruit
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1007/s11240-015-0824-1 http://www.locus.ufv.br/handle/123456789/21837 |
Resumo: | Micrografting, used to eliminate viruses, involves the utilization of very small grafts, and detailed structural analyses of the micrografting region in different phases are presented here. Shoot tips with 2–3 leaf primordia, and 600–800 µm in length, were grafted to the hypocotyl of 21–28 day-old rootstock seedlings, and their development was followed for 30 days using scanning electron and visible light microscopy. The success of micrografting was found to depend on the preservation of the vascular tissue of the rootstock and the placement of the scion adjacent to the rootstock phloem. Callus formation, which initiates approximately 3 days after micrografting (DAM) through the proliferation of parenchymatous cells of the rootstock cortex, fills the space left by the incision and guarantees adherence and nutrition for the scion during the initial phases of development. At seven DAM, connective cells that develop at the base of the scion produce a junction with the callus. At 10 DAM, differentiation of procambial strands and parenchymatous cells initiates in the callus. At 15 DAM, parenchymatous cells derived from the callus give rise to procambial strands and initiate the differentiation of tracheal elements and the epidermis in the junction region. Vascular connections are established at 20 DAM, promoting the accelerated development of the scion, which, at 30 DAM, shows shoot development. The developmental phases of micrografting in passionfruit plants therefore include: placement of the scion; callus formation by the rootstock; cellular connections; differentiation of the callus; vascular connection; and shoot development. |
id |
UFV_add7b174659eac5c13504c20e2ff3b80 |
---|---|
oai_identifier_str |
oai:locus.ufv.br:123456789/21837 |
network_acronym_str |
UFV |
network_name_str |
LOCUS Repositório Institucional da UFV |
repository_id_str |
2145 |
spelling |
Nery, Lays AraújoRibeiro, Leonardo MonteiroVieira, Lorena MeloMercadante-Simões, Maria Olívia2018-09-17T11:00:35Z2018-09-17T11:00:35Z2015-07-2315735044http://dx.doi.org/10.1007/s11240-015-0824-1http://www.locus.ufv.br/handle/123456789/21837Micrografting, used to eliminate viruses, involves the utilization of very small grafts, and detailed structural analyses of the micrografting region in different phases are presented here. Shoot tips with 2–3 leaf primordia, and 600–800 µm in length, were grafted to the hypocotyl of 21–28 day-old rootstock seedlings, and their development was followed for 30 days using scanning electron and visible light microscopy. The success of micrografting was found to depend on the preservation of the vascular tissue of the rootstock and the placement of the scion adjacent to the rootstock phloem. Callus formation, which initiates approximately 3 days after micrografting (DAM) through the proliferation of parenchymatous cells of the rootstock cortex, fills the space left by the incision and guarantees adherence and nutrition for the scion during the initial phases of development. At seven DAM, connective cells that develop at the base of the scion produce a junction with the callus. At 10 DAM, differentiation of procambial strands and parenchymatous cells initiates in the callus. At 15 DAM, parenchymatous cells derived from the callus give rise to procambial strands and initiate the differentiation of tracheal elements and the epidermis in the junction region. Vascular connections are established at 20 DAM, promoting the accelerated development of the scion, which, at 30 DAM, shows shoot development. The developmental phases of micrografting in passionfruit plants therefore include: placement of the scion; callus formation by the rootstock; cellular connections; differentiation of the callus; vascular connection; and shoot development.engPlant Cell, Tissue and Organ Culture (PCTOC)v. 123, n. 1, p. 173– 181, out. 2015Springer Nature Switzerland AG.info:eu-repo/semantics/openAccessPassiflora edulis SimsScionRootstockShoot-tip graftingCallus formationVascular connectionHistological study of micrografting in passionfruitinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf2976901https://locus.ufv.br//bitstream/123456789/21837/1/artigo.pdf599e02f7d26cdbd8f608e010df30cd71MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/21837/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5038https://locus.ufv.br//bitstream/123456789/21837/3/artigo.pdf.jpg6fe20aa1d1ad10a7ea19beaf31286995MD53123456789/218372018-09-17 23:00:26.984oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-09-18T02:00:26LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Histological study of micrografting in passionfruit |
title |
Histological study of micrografting in passionfruit |
spellingShingle |
Histological study of micrografting in passionfruit Nery, Lays Araújo Passiflora edulis Sims Scion Rootstock Shoot-tip grafting Callus formation Vascular connection |
title_short |
Histological study of micrografting in passionfruit |
title_full |
Histological study of micrografting in passionfruit |
title_fullStr |
Histological study of micrografting in passionfruit |
title_full_unstemmed |
Histological study of micrografting in passionfruit |
title_sort |
Histological study of micrografting in passionfruit |
author |
Nery, Lays Araújo |
author_facet |
Nery, Lays Araújo Ribeiro, Leonardo Monteiro Vieira, Lorena Melo Mercadante-Simões, Maria Olívia |
author_role |
author |
author2 |
Ribeiro, Leonardo Monteiro Vieira, Lorena Melo Mercadante-Simões, Maria Olívia |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Nery, Lays Araújo Ribeiro, Leonardo Monteiro Vieira, Lorena Melo Mercadante-Simões, Maria Olívia |
dc.subject.pt-BR.fl_str_mv |
Passiflora edulis Sims Scion Rootstock Shoot-tip grafting Callus formation Vascular connection |
topic |
Passiflora edulis Sims Scion Rootstock Shoot-tip grafting Callus formation Vascular connection |
description |
Micrografting, used to eliminate viruses, involves the utilization of very small grafts, and detailed structural analyses of the micrografting region in different phases are presented here. Shoot tips with 2–3 leaf primordia, and 600–800 µm in length, were grafted to the hypocotyl of 21–28 day-old rootstock seedlings, and their development was followed for 30 days using scanning electron and visible light microscopy. The success of micrografting was found to depend on the preservation of the vascular tissue of the rootstock and the placement of the scion adjacent to the rootstock phloem. Callus formation, which initiates approximately 3 days after micrografting (DAM) through the proliferation of parenchymatous cells of the rootstock cortex, fills the space left by the incision and guarantees adherence and nutrition for the scion during the initial phases of development. At seven DAM, connective cells that develop at the base of the scion produce a junction with the callus. At 10 DAM, differentiation of procambial strands and parenchymatous cells initiates in the callus. At 15 DAM, parenchymatous cells derived from the callus give rise to procambial strands and initiate the differentiation of tracheal elements and the epidermis in the junction region. Vascular connections are established at 20 DAM, promoting the accelerated development of the scion, which, at 30 DAM, shows shoot development. The developmental phases of micrografting in passionfruit plants therefore include: placement of the scion; callus formation by the rootstock; cellular connections; differentiation of the callus; vascular connection; and shoot development. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-07-23 |
dc.date.accessioned.fl_str_mv |
2018-09-17T11:00:35Z |
dc.date.available.fl_str_mv |
2018-09-17T11:00:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11240-015-0824-1 http://www.locus.ufv.br/handle/123456789/21837 |
dc.identifier.issn.none.fl_str_mv |
15735044 |
identifier_str_mv |
15735044 |
url |
http://dx.doi.org/10.1007/s11240-015-0824-1 http://www.locus.ufv.br/handle/123456789/21837 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 123, n. 1, p. 173– 181, out. 2015 |
dc.rights.driver.fl_str_mv |
Springer Nature Switzerland AG. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Springer Nature Switzerland AG. |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Plant Cell, Tissue and Organ Culture (PCTOC) |
publisher.none.fl_str_mv |
Plant Cell, Tissue and Organ Culture (PCTOC) |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
bitstream.url.fl_str_mv |
https://locus.ufv.br//bitstream/123456789/21837/1/artigo.pdf https://locus.ufv.br//bitstream/123456789/21837/2/license.txt https://locus.ufv.br//bitstream/123456789/21837/3/artigo.pdf.jpg |
bitstream.checksum.fl_str_mv |
599e02f7d26cdbd8f608e010df30cd71 8a4605be74aa9ea9d79846c1fba20a33 6fe20aa1d1ad10a7ea19beaf31286995 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1801213061695012864 |