Genetic variability and population structure in Melipona scutellaris (Hymenoptera: Apidae) from Bahia, Brazil, based on molecular markers
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1007/s13592-013-0220-y http://www.locus.ufv.br/handle/123456789/24100 |
Resumo: | Melipona scutellaris is an important pollinator in natural and cultured areas from northeastern Brazil. Therefore, the goal of this work was to investigate the genetic diversity and population structure of M. scutellaris within its Bahia range, relating putative geographical influences on population dynamics. A total of 111 colonies from 13 municipalities in Bahia from sea level up to 1,011 m of altitude were sampled. Five species-specific codominant (microsatellites) and ten dominant (ISSR) primers were amplified, yielding from 2 to 13 alleles and 94 bands, respectively. The mean genetic diversity (He) was 0.50 for microsatellites and 0.33 for ISSR markers. AMOVA revealed that most of genetic variation is found within localities (82.6 % for microsatellites and 73.6 % for ISSR), and UPGMA and Bayesian analysis revealed the formation of two genetic groups related to altitude. Therefore, conservation strategies should take altitude variation into consideration to assure the genetic integrity of M. scutellaris. |
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Tavares, Mara GarciaPassamani, Paulo Z.Resende, Helder C.Campos, Lucio Antonio de O.Almeida, Bruno S.Paiva, Samuel R.Alves, Rogério Marcos de O.Waldschmidt, Ana M.2019-03-25T17:05:26Z2019-03-25T17:05:26Z2013-111297-9678https://doi.org/10.1007/s13592-013-0220-yhttp://www.locus.ufv.br/handle/123456789/24100Melipona scutellaris is an important pollinator in natural and cultured areas from northeastern Brazil. Therefore, the goal of this work was to investigate the genetic diversity and population structure of M. scutellaris within its Bahia range, relating putative geographical influences on population dynamics. A total of 111 colonies from 13 municipalities in Bahia from sea level up to 1,011 m of altitude were sampled. Five species-specific codominant (microsatellites) and ten dominant (ISSR) primers were amplified, yielding from 2 to 13 alleles and 94 bands, respectively. The mean genetic diversity (He) was 0.50 for microsatellites and 0.33 for ISSR markers. AMOVA revealed that most of genetic variation is found within localities (82.6 % for microsatellites and 73.6 % for ISSR), and UPGMA and Bayesian analysis revealed the formation of two genetic groups related to altitude. Therefore, conservation strategies should take altitude variation into consideration to assure the genetic integrity of M. scutellaris.engApidologieVolume 44, Issue 6, Pages 720–728, November 2013INRA, DIB and Springer-Verlag Franceinfo:eu-repo/semantics/openAccessConservationISSRMicrosatellitesPopulation geneticsStingless beeGenetic variability and population structure in Melipona scutellaris (Hymenoptera: Apidae) from Bahia, Brazil, based on molecular markersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf238976https://locus.ufv.br//bitstream/123456789/24100/1/artigo.pdfd5ab823b4fa4c175d1ae9ffe9ffa7141MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/24100/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/241002019-03-25 14:07:00.37oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-03-25T17:07LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Genetic variability and population structure in Melipona scutellaris (Hymenoptera: Apidae) from Bahia, Brazil, based on molecular markers |
title |
Genetic variability and population structure in Melipona scutellaris (Hymenoptera: Apidae) from Bahia, Brazil, based on molecular markers |
spellingShingle |
Genetic variability and population structure in Melipona scutellaris (Hymenoptera: Apidae) from Bahia, Brazil, based on molecular markers Tavares, Mara Garcia Conservation ISSR Microsatellites Population genetics Stingless bee |
title_short |
Genetic variability and population structure in Melipona scutellaris (Hymenoptera: Apidae) from Bahia, Brazil, based on molecular markers |
title_full |
Genetic variability and population structure in Melipona scutellaris (Hymenoptera: Apidae) from Bahia, Brazil, based on molecular markers |
title_fullStr |
Genetic variability and population structure in Melipona scutellaris (Hymenoptera: Apidae) from Bahia, Brazil, based on molecular markers |
title_full_unstemmed |
Genetic variability and population structure in Melipona scutellaris (Hymenoptera: Apidae) from Bahia, Brazil, based on molecular markers |
title_sort |
Genetic variability and population structure in Melipona scutellaris (Hymenoptera: Apidae) from Bahia, Brazil, based on molecular markers |
author |
Tavares, Mara Garcia |
author_facet |
Tavares, Mara Garcia Passamani, Paulo Z. Resende, Helder C. Campos, Lucio Antonio de O. Almeida, Bruno S. Paiva, Samuel R. Alves, Rogério Marcos de O. Waldschmidt, Ana M. |
author_role |
author |
author2 |
Passamani, Paulo Z. Resende, Helder C. Campos, Lucio Antonio de O. Almeida, Bruno S. Paiva, Samuel R. Alves, Rogério Marcos de O. Waldschmidt, Ana M. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Tavares, Mara Garcia Passamani, Paulo Z. Resende, Helder C. Campos, Lucio Antonio de O. Almeida, Bruno S. Paiva, Samuel R. Alves, Rogério Marcos de O. Waldschmidt, Ana M. |
dc.subject.pt-BR.fl_str_mv |
Conservation ISSR Microsatellites Population genetics Stingless bee |
topic |
Conservation ISSR Microsatellites Population genetics Stingless bee |
description |
Melipona scutellaris is an important pollinator in natural and cultured areas from northeastern Brazil. Therefore, the goal of this work was to investigate the genetic diversity and population structure of M. scutellaris within its Bahia range, relating putative geographical influences on population dynamics. A total of 111 colonies from 13 municipalities in Bahia from sea level up to 1,011 m of altitude were sampled. Five species-specific codominant (microsatellites) and ten dominant (ISSR) primers were amplified, yielding from 2 to 13 alleles and 94 bands, respectively. The mean genetic diversity (He) was 0.50 for microsatellites and 0.33 for ISSR markers. AMOVA revealed that most of genetic variation is found within localities (82.6 % for microsatellites and 73.6 % for ISSR), and UPGMA and Bayesian analysis revealed the formation of two genetic groups related to altitude. Therefore, conservation strategies should take altitude variation into consideration to assure the genetic integrity of M. scutellaris. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-11 |
dc.date.accessioned.fl_str_mv |
2019-03-25T17:05:26Z |
dc.date.available.fl_str_mv |
2019-03-25T17:05:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1007/s13592-013-0220-y http://www.locus.ufv.br/handle/123456789/24100 |
dc.identifier.issn.none.fl_str_mv |
1297-9678 |
identifier_str_mv |
1297-9678 |
url |
https://doi.org/10.1007/s13592-013-0220-y http://www.locus.ufv.br/handle/123456789/24100 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
Volume 44, Issue 6, Pages 720–728, November 2013 |
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INRA, DIB and Springer-Verlag France info:eu-repo/semantics/openAccess |
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INRA, DIB and Springer-Verlag France |
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openAccess |
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application/pdf |
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Apidologie |
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Apidologie |
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