In vitro responses in Passiflora species with different chromosome numbers, ploidy levels and nuclear 2C values: revisiting and providing new insights
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1007/s11240-018-01536-9 http://www.locus.ufv.br/handle/123456789/23880 |
Resumo: | Tissue culture in Passiflora has emerged as a strategy to propagate species with agronomic relevance, which is the main focus of most in vitro studies. Different morphogenic responses have been obtained under the same environmental in vitro conditions, mainly for species of the subgenus Passiflora with distinct 2n chromosome numbers. The aims of this study were to verify and compare the in vitro responses in Passiflora species with distinct 2n chromosome numbers, ploidy levels and nuclear 2C values. Under the same in vitro conditions, only friable calli occurred from mature zygotic embryo explants of Passiflora coriacea (2n = 2x = 12 chromosomes, 2C = 1.00 pg), Passiflora lindeniana (2n = 4x = 24, 2C = 2.42 pg) and Passiflora contracta (2n = 8x = 48, 2C = 4.78 pg). In contrast, plantlets were regenerated from Passiflora foetida (2n = 20, 2C = 1.04 pg) and Passiflora miniata (2n = 18, 2C = 3.40 pg) via indirect organogenesis and indirect somatic embryogenesis, respectively. By now, from mature zygotic embryo explants, de novo shoot organogenesis and somatic embryos have been recovered for Passiflora species with 2n = 18 chromosomes and relative high nuclear 2C value (more than 2C = 2.93 pg—Passiflora cincinata), and only de novo shoot organogenesis for P. foetida with 2n = 20 chromosomes and relative low 2C value (2C = 1.04 pg). Therefore, in a taxonomic and evolutive context, this study showed that the in vitro morphogenic pathways pretty varied between the Passiflora species with distinct karyotype features. |
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Carvalho, Carlos RobertoClarindo, Wellington RonildoFerreira, AdésioPraça-Fontes, Milene MirandaVieira, Ariane TonettoFerreira, Darley Aparecido TavaresLeite, Cristiana Torres2019-03-12T14:29:05Z2019-03-12T14:29:05Z2019-031573-5044https://doi.org/10.1007/s11240-018-01536-9http://www.locus.ufv.br/handle/123456789/23880Tissue culture in Passiflora has emerged as a strategy to propagate species with agronomic relevance, which is the main focus of most in vitro studies. Different morphogenic responses have been obtained under the same environmental in vitro conditions, mainly for species of the subgenus Passiflora with distinct 2n chromosome numbers. The aims of this study were to verify and compare the in vitro responses in Passiflora species with distinct 2n chromosome numbers, ploidy levels and nuclear 2C values. Under the same in vitro conditions, only friable calli occurred from mature zygotic embryo explants of Passiflora coriacea (2n = 2x = 12 chromosomes, 2C = 1.00 pg), Passiflora lindeniana (2n = 4x = 24, 2C = 2.42 pg) and Passiflora contracta (2n = 8x = 48, 2C = 4.78 pg). In contrast, plantlets were regenerated from Passiflora foetida (2n = 20, 2C = 1.04 pg) and Passiflora miniata (2n = 18, 2C = 3.40 pg) via indirect organogenesis and indirect somatic embryogenesis, respectively. By now, from mature zygotic embryo explants, de novo shoot organogenesis and somatic embryos have been recovered for Passiflora species with 2n = 18 chromosomes and relative high nuclear 2C value (more than 2C = 2.93 pg—Passiflora cincinata), and only de novo shoot organogenesis for P. foetida with 2n = 20 chromosomes and relative low 2C value (2C = 1.04 pg). Therefore, in a taxonomic and evolutive context, this study showed that the in vitro morphogenic pathways pretty varied between the Passiflora species with distinct karyotype features.engPlant Cell, Tissue and Organ Culture (PCTOC)Volume 136, Issue 3, Pages 549–560, March 2019Springer Nature B.V.info:eu-repo/semantics/openAccessIndirect somatic embryogenesisIndirect organogenesisKaryotypePloidy levelNuclearDNA contentPassion fruitTissue cultureIn vitro responses in Passiflora species with different chromosome numbers, ploidy levels and nuclear 2C values: revisiting and providing new insightsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf3843389https://locus.ufv.br//bitstream/123456789/23880/1/artigo.pdfc9cf1e7c98ce90bb44d7349f7822e548MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/23880/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/238802019-03-12 11:31:36.388oai:locus.ufv.br:123456789/23880Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-03-12T14:31:36LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
In vitro responses in Passiflora species with different chromosome numbers, ploidy levels and nuclear 2C values: revisiting and providing new insights |
title |
In vitro responses in Passiflora species with different chromosome numbers, ploidy levels and nuclear 2C values: revisiting and providing new insights |
spellingShingle |
In vitro responses in Passiflora species with different chromosome numbers, ploidy levels and nuclear 2C values: revisiting and providing new insights Carvalho, Carlos Roberto Indirect somatic embryogenesis Indirect organogenesis Karyotype Ploidy level Nuclear DNA content Passion fruit Tissue culture |
title_short |
In vitro responses in Passiflora species with different chromosome numbers, ploidy levels and nuclear 2C values: revisiting and providing new insights |
title_full |
In vitro responses in Passiflora species with different chromosome numbers, ploidy levels and nuclear 2C values: revisiting and providing new insights |
title_fullStr |
In vitro responses in Passiflora species with different chromosome numbers, ploidy levels and nuclear 2C values: revisiting and providing new insights |
title_full_unstemmed |
In vitro responses in Passiflora species with different chromosome numbers, ploidy levels and nuclear 2C values: revisiting and providing new insights |
title_sort |
In vitro responses in Passiflora species with different chromosome numbers, ploidy levels and nuclear 2C values: revisiting and providing new insights |
author |
Carvalho, Carlos Roberto |
author_facet |
Carvalho, Carlos Roberto Clarindo, Wellington Ronildo Ferreira, Adésio Praça-Fontes, Milene Miranda Vieira, Ariane Tonetto Ferreira, Darley Aparecido Tavares Leite, Cristiana Torres |
author_role |
author |
author2 |
Clarindo, Wellington Ronildo Ferreira, Adésio Praça-Fontes, Milene Miranda Vieira, Ariane Tonetto Ferreira, Darley Aparecido Tavares Leite, Cristiana Torres |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Carvalho, Carlos Roberto Clarindo, Wellington Ronildo Ferreira, Adésio Praça-Fontes, Milene Miranda Vieira, Ariane Tonetto Ferreira, Darley Aparecido Tavares Leite, Cristiana Torres |
dc.subject.pt-BR.fl_str_mv |
Indirect somatic embryogenesis Indirect organogenesis Karyotype Ploidy level Nuclear DNA content Passion fruit Tissue culture |
topic |
Indirect somatic embryogenesis Indirect organogenesis Karyotype Ploidy level Nuclear DNA content Passion fruit Tissue culture |
description |
Tissue culture in Passiflora has emerged as a strategy to propagate species with agronomic relevance, which is the main focus of most in vitro studies. Different morphogenic responses have been obtained under the same environmental in vitro conditions, mainly for species of the subgenus Passiflora with distinct 2n chromosome numbers. The aims of this study were to verify and compare the in vitro responses in Passiflora species with distinct 2n chromosome numbers, ploidy levels and nuclear 2C values. Under the same in vitro conditions, only friable calli occurred from mature zygotic embryo explants of Passiflora coriacea (2n = 2x = 12 chromosomes, 2C = 1.00 pg), Passiflora lindeniana (2n = 4x = 24, 2C = 2.42 pg) and Passiflora contracta (2n = 8x = 48, 2C = 4.78 pg). In contrast, plantlets were regenerated from Passiflora foetida (2n = 20, 2C = 1.04 pg) and Passiflora miniata (2n = 18, 2C = 3.40 pg) via indirect organogenesis and indirect somatic embryogenesis, respectively. By now, from mature zygotic embryo explants, de novo shoot organogenesis and somatic embryos have been recovered for Passiflora species with 2n = 18 chromosomes and relative high nuclear 2C value (more than 2C = 2.93 pg—Passiflora cincinata), and only de novo shoot organogenesis for P. foetida with 2n = 20 chromosomes and relative low 2C value (2C = 1.04 pg). Therefore, in a taxonomic and evolutive context, this study showed that the in vitro morphogenic pathways pretty varied between the Passiflora species with distinct karyotype features. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-03-12T14:29:05Z |
dc.date.available.fl_str_mv |
2019-03-12T14:29:05Z |
dc.date.issued.fl_str_mv |
2019-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1007/s11240-018-01536-9 http://www.locus.ufv.br/handle/123456789/23880 |
dc.identifier.issn.none.fl_str_mv |
1573-5044 |
identifier_str_mv |
1573-5044 |
url |
https://doi.org/10.1007/s11240-018-01536-9 http://www.locus.ufv.br/handle/123456789/23880 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
Volume 136, Issue 3, Pages 549–560, March 2019 |
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Springer Nature B.V. info:eu-repo/semantics/openAccess |
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Springer Nature B.V. |
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openAccess |
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Plant Cell, Tissue and Organ Culture (PCTOC) |
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Plant Cell, Tissue and Organ Culture (PCTOC) |
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