Development of a selective culture medium for bifidobacteria, Raffinose-Propionate Lithium Mupirocin (RP-MUP) and assessment of its usage with PetrifilmÔ Aerobic Count plates
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.fm.2013.11.010 http://www.locus.ufv.br/handle/123456789/13995 |
Resumo: | This study aimed to develop a selective culture media to enumerate bifidobacteria in fermented milk and to assess this medium when used with PetrifilmÔ AC plates. For this purpose, Bifidobacterium spp., Lactobacillus spp. and Streptococcus thermophilus strains were tested to verify their fermentation patterns for different carbohydrates. All bifidobacteria strains were able to use raffinose. Based on these characteristic, a selective culture medium was proposed (Raffinose-Propionate Lithium Mupirocin, RP-MUP), used with PetrifilmÔ AC plates, and was used to enumerate bifidobacteria in fermented milk. RP-MUP performance was assessed by comparing the results with this medium to reference protocols and culture media for bifidobacteria enumeration. RP-MUP, whether used or not with PetrifilmÔ AC, presented similar performance to TOS-MUP (ISO 29981), with no significant differences between the mean bifi dobacteria counts (p < 0.05) and with high correlation indices (r 1⁄4 0.99, p < 0.05). As an advantage, reliable results were obtained after just 48 h of incubation when RP-MUP was used with PetrifilmÔ AC, instead of the 72 h described in the ISO 29981 protocol. |
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Miranda, Rodrigo OtávioCarvalho, Antonio Fernandes deNero, Luís Augusto2017-11-29T16:25:18Z2017-11-29T16:25:18Z2013-11-2607400020https://doi.org/10.1016/j.fm.2013.11.010http://www.locus.ufv.br/handle/123456789/13995This study aimed to develop a selective culture media to enumerate bifidobacteria in fermented milk and to assess this medium when used with PetrifilmÔ AC plates. For this purpose, Bifidobacterium spp., Lactobacillus spp. and Streptococcus thermophilus strains were tested to verify their fermentation patterns for different carbohydrates. All bifidobacteria strains were able to use raffinose. Based on these characteristic, a selective culture medium was proposed (Raffinose-Propionate Lithium Mupirocin, RP-MUP), used with PetrifilmÔ AC plates, and was used to enumerate bifidobacteria in fermented milk. RP-MUP performance was assessed by comparing the results with this medium to reference protocols and culture media for bifidobacteria enumeration. RP-MUP, whether used or not with PetrifilmÔ AC, presented similar performance to TOS-MUP (ISO 29981), with no significant differences between the mean bifi dobacteria counts (p < 0.05) and with high correlation indices (r 1⁄4 0.99, p < 0.05). As an advantage, reliable results were obtained after just 48 h of incubation when RP-MUP was used with PetrifilmÔ AC, instead of the 72 h described in the ISO 29981 protocol.engFood MicrobiologyVolume 39, Pages 96-102, May 2014BifidobacteriaSelective enumerationPetrifilm ACTOS-MUPRP-MUPDevelopment of a selective culture medium for bifidobacteria, Raffinose-Propionate Lithium Mupirocin (RP-MUP) and assessment of its usage with PetrifilmÔ Aerobic Count platesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINAL1-s2.0-S0740002013002311-main.pdf1-s2.0-S0740002013002311-main.pdftexto completoapplication/pdf494876https://locus.ufv.br//bitstream/123456789/13995/1/1-s2.0-S0740002013002311-main.pdfc833f5ebb236b1911b4915b56cd0a80aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/13995/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAIL1-s2.0-S0740002013002311-main.pdf.jpg1-s2.0-S0740002013002311-main.pdf.jpgIM Thumbnailimage/jpeg6426https://locus.ufv.br//bitstream/123456789/13995/3/1-s2.0-S0740002013002311-main.pdf.jpg8a4eaa152fedadcee4dd6349afd3aaf2MD53123456789/139952017-11-29 22:00:38.074oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-11-30T01:00:38LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Development of a selective culture medium for bifidobacteria, Raffinose-Propionate Lithium Mupirocin (RP-MUP) and assessment of its usage with PetrifilmÔ Aerobic Count plates |
title |
Development of a selective culture medium for bifidobacteria, Raffinose-Propionate Lithium Mupirocin (RP-MUP) and assessment of its usage with PetrifilmÔ Aerobic Count plates |
spellingShingle |
Development of a selective culture medium for bifidobacteria, Raffinose-Propionate Lithium Mupirocin (RP-MUP) and assessment of its usage with PetrifilmÔ Aerobic Count plates Miranda, Rodrigo Otávio Bifidobacteria Selective enumeration Petrifilm AC TOS-MUP RP-MUP |
title_short |
Development of a selective culture medium for bifidobacteria, Raffinose-Propionate Lithium Mupirocin (RP-MUP) and assessment of its usage with PetrifilmÔ Aerobic Count plates |
title_full |
Development of a selective culture medium for bifidobacteria, Raffinose-Propionate Lithium Mupirocin (RP-MUP) and assessment of its usage with PetrifilmÔ Aerobic Count plates |
title_fullStr |
Development of a selective culture medium for bifidobacteria, Raffinose-Propionate Lithium Mupirocin (RP-MUP) and assessment of its usage with PetrifilmÔ Aerobic Count plates |
title_full_unstemmed |
Development of a selective culture medium for bifidobacteria, Raffinose-Propionate Lithium Mupirocin (RP-MUP) and assessment of its usage with PetrifilmÔ Aerobic Count plates |
title_sort |
Development of a selective culture medium for bifidobacteria, Raffinose-Propionate Lithium Mupirocin (RP-MUP) and assessment of its usage with PetrifilmÔ Aerobic Count plates |
author |
Miranda, Rodrigo Otávio |
author_facet |
Miranda, Rodrigo Otávio Carvalho, Antonio Fernandes de Nero, Luís Augusto |
author_role |
author |
author2 |
Carvalho, Antonio Fernandes de Nero, Luís Augusto |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Miranda, Rodrigo Otávio Carvalho, Antonio Fernandes de Nero, Luís Augusto |
dc.subject.pt-BR.fl_str_mv |
Bifidobacteria Selective enumeration Petrifilm AC TOS-MUP RP-MUP |
topic |
Bifidobacteria Selective enumeration Petrifilm AC TOS-MUP RP-MUP |
description |
This study aimed to develop a selective culture media to enumerate bifidobacteria in fermented milk and to assess this medium when used with PetrifilmÔ AC plates. For this purpose, Bifidobacterium spp., Lactobacillus spp. and Streptococcus thermophilus strains were tested to verify their fermentation patterns for different carbohydrates. All bifidobacteria strains were able to use raffinose. Based on these characteristic, a selective culture medium was proposed (Raffinose-Propionate Lithium Mupirocin, RP-MUP), used with PetrifilmÔ AC plates, and was used to enumerate bifidobacteria in fermented milk. RP-MUP performance was assessed by comparing the results with this medium to reference protocols and culture media for bifidobacteria enumeration. RP-MUP, whether used or not with PetrifilmÔ AC, presented similar performance to TOS-MUP (ISO 29981), with no significant differences between the mean bifi dobacteria counts (p < 0.05) and with high correlation indices (r 1⁄4 0.99, p < 0.05). As an advantage, reliable results were obtained after just 48 h of incubation when RP-MUP was used with PetrifilmÔ AC, instead of the 72 h described in the ISO 29981 protocol. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-11-26 |
dc.date.accessioned.fl_str_mv |
2017-11-29T16:25:18Z |
dc.date.available.fl_str_mv |
2017-11-29T16:25:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.fm.2013.11.010 http://www.locus.ufv.br/handle/123456789/13995 |
dc.identifier.issn.none.fl_str_mv |
07400020 |
identifier_str_mv |
07400020 |
url |
https://doi.org/10.1016/j.fm.2013.11.010 http://www.locus.ufv.br/handle/123456789/13995 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
Volume 39, Pages 96-102, May 2014 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Food Microbiology |
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Food Microbiology |
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LOCUS Repositório Institucional da UFV |
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