Comparison of GC and HPLC for the quantification of organic acids in coffee
Autor(a) principal: | |
---|---|
Data de Publicação: | 2001 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1002/pca.629 http://www.locus.ufv.br/handle/123456789/14974 |
Resumo: | A GC and an HPLC method for the quantification of organic acids OAs in coffee have been compared. The GC procedure, employing trimethylsilyl derivatives, was found to be very tedious. The HPLC method, which employed an ion exchange column using a flow gradient of water containing 1% phosphoric acid and UV detection (210 nm), was found to be much simpler for the quantification of eight organic acids (oxalic, succinic, fumaric, malic, tartaric, citric, quinic and fumaric acids) in four representative coffee samples. The HPLC procedure was more convenient than that described in the literature since no pre-purification was required for quantification of the OAs. |
id |
UFV_dd1032a0988199f94914994092e54343 |
---|---|
oai_identifier_str |
oai:locus.ufv.br:123456789/14974 |
network_acronym_str |
UFV |
network_name_str |
LOCUS Repositório Institucional da UFV |
repository_id_str |
2145 |
spelling |
Jham, Gulab N.Fernande, Sergio A.Garcia, Cleverson FernandoSilva, Alexsandro Araujo da2017-12-13T17:11:07Z2017-12-13T17:11:07Z2001-05-161099-1565http://dx.doi.org/10.1002/pca.629http://www.locus.ufv.br/handle/123456789/14974A GC and an HPLC method for the quantification of organic acids OAs in coffee have been compared. The GC procedure, employing trimethylsilyl derivatives, was found to be very tedious. The HPLC method, which employed an ion exchange column using a flow gradient of water containing 1% phosphoric acid and UV detection (210 nm), was found to be much simpler for the quantification of eight organic acids (oxalic, succinic, fumaric, malic, tartaric, citric, quinic and fumaric acids) in four representative coffee samples. The HPLC procedure was more convenient than that described in the literature since no pre-purification was required for quantification of the OAs.engPhytochemical Analysis13(2), p. 99-104, Mar.-Apr. 2002GCHPLCQuantitative determinationOrganic acidsCoffeeComparison of GC and HPLC for the quantification of organic acids in coffeeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALJham_et_al-2002-Phytochemical_Analysis.pdfJham_et_al-2002-Phytochemical_Analysis.pdftexto completoapplication/pdf88069https://locus.ufv.br//bitstream/123456789/14974/1/Jham_et_al-2002-Phytochemical_Analysis.pdfe5031745b2b137aca7190ec0b212f920MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/14974/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILJham_et_al-2002-Phytochemical_Analysis.pdf.jpgJham_et_al-2002-Phytochemical_Analysis.pdf.jpgIM Thumbnailimage/jpeg5233https://locus.ufv.br//bitstream/123456789/14974/3/Jham_et_al-2002-Phytochemical_Analysis.pdf.jpg2c52d341272c400415e81b638c129538MD53123456789/149742017-12-13 22:00:52.918oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-12-14T01:00:52LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Comparison of GC and HPLC for the quantification of organic acids in coffee |
title |
Comparison of GC and HPLC for the quantification of organic acids in coffee |
spellingShingle |
Comparison of GC and HPLC for the quantification of organic acids in coffee Jham, Gulab N. GC HPLC Quantitative determination Organic acids Coffee |
title_short |
Comparison of GC and HPLC for the quantification of organic acids in coffee |
title_full |
Comparison of GC and HPLC for the quantification of organic acids in coffee |
title_fullStr |
Comparison of GC and HPLC for the quantification of organic acids in coffee |
title_full_unstemmed |
Comparison of GC and HPLC for the quantification of organic acids in coffee |
title_sort |
Comparison of GC and HPLC for the quantification of organic acids in coffee |
author |
Jham, Gulab N. |
author_facet |
Jham, Gulab N. Fernande, Sergio A. Garcia, Cleverson Fernando Silva, Alexsandro Araujo da |
author_role |
author |
author2 |
Fernande, Sergio A. Garcia, Cleverson Fernando Silva, Alexsandro Araujo da |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Jham, Gulab N. Fernande, Sergio A. Garcia, Cleverson Fernando Silva, Alexsandro Araujo da |
dc.subject.pt-BR.fl_str_mv |
GC HPLC Quantitative determination Organic acids Coffee |
topic |
GC HPLC Quantitative determination Organic acids Coffee |
description |
A GC and an HPLC method for the quantification of organic acids OAs in coffee have been compared. The GC procedure, employing trimethylsilyl derivatives, was found to be very tedious. The HPLC method, which employed an ion exchange column using a flow gradient of water containing 1% phosphoric acid and UV detection (210 nm), was found to be much simpler for the quantification of eight organic acids (oxalic, succinic, fumaric, malic, tartaric, citric, quinic and fumaric acids) in four representative coffee samples. The HPLC procedure was more convenient than that described in the literature since no pre-purification was required for quantification of the OAs. |
publishDate |
2001 |
dc.date.issued.fl_str_mv |
2001-05-16 |
dc.date.accessioned.fl_str_mv |
2017-12-13T17:11:07Z |
dc.date.available.fl_str_mv |
2017-12-13T17:11:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/pca.629 http://www.locus.ufv.br/handle/123456789/14974 |
dc.identifier.issn.none.fl_str_mv |
1099-1565 |
identifier_str_mv |
1099-1565 |
url |
http://dx.doi.org/10.1002/pca.629 http://www.locus.ufv.br/handle/123456789/14974 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
13(2), p. 99-104, Mar.-Apr. 2002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Phytochemical Analysis |
publisher.none.fl_str_mv |
Phytochemical Analysis |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
bitstream.url.fl_str_mv |
https://locus.ufv.br//bitstream/123456789/14974/1/Jham_et_al-2002-Phytochemical_Analysis.pdf https://locus.ufv.br//bitstream/123456789/14974/2/license.txt https://locus.ufv.br//bitstream/123456789/14974/3/Jham_et_al-2002-Phytochemical_Analysis.pdf.jpg |
bitstream.checksum.fl_str_mv |
e5031745b2b137aca7190ec0b212f920 8a4605be74aa9ea9d79846c1fba20a33 2c52d341272c400415e81b638c129538 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1801213001067397120 |