In silico characterization of 1,2-diacylglycerol cholinephosphotransferase and lysophospha-tidylcholine acyltransferase genes in Glycine max L. Merrill.
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Publication Date: | 2016 |
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Format: | Article |
Language: | eng |
Source: | LOCUS Repositório Institucional da UFV |
Download full: | http://dx.doi.org/10.4238/gmr.15038974 http://www.locus.ufv.br/handle/123456789/12561 |
Summary: | The enzymes 1,2-diacylglycerol cholinephosphotrans-ferase (CPT) and lysophosphatidylcholine acyltransferase (LPCAT) are important in lipid metabolism in soybean seeds. Thus, understand-ing the genes that encode these enzymes may enable their modification and aid the improvement of soybean oil quality. In soybean, the genes encoding these enzymes have not been completely described; there-fore, this study aimed to identify, characterize, and analyze the in silico expression of these genes in soybean. We identified two gene models encoding CPT and two gene models encoding LPCAT, one of which presented an alternative transcript. The sequences were positioned on the physical map of soybean and the promoter regions were analyzed. Cis-elements responsible for seed-specific expression and responses to biotic and abiotic stresses were identified. Virtual expression analysis of the gene models for CPT and LPCAT indicated that these genes are expressed under different stress conditions, in somatic embryos during differentiation, in immature seeds, root tissues, and calli. Putative ami-no acid sequences revealed the presence of transmembrane domains, and analysis of the cellular localization of these enzymes revealed they are located in the endoplasmic reticulum. |
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Sousa, C.S.Barros, E.G.Moreira, M.A.Barros, B.A.Barh, D.Ghosh, P.Azevedo, V.2017-10-31T09:36:03Z2017-10-31T09:36:03Z2016-08-2616765680http://dx.doi.org/10.4238/gmr.15038974http://www.locus.ufv.br/handle/123456789/12561The enzymes 1,2-diacylglycerol cholinephosphotrans-ferase (CPT) and lysophosphatidylcholine acyltransferase (LPCAT) are important in lipid metabolism in soybean seeds. Thus, understand-ing the genes that encode these enzymes may enable their modification and aid the improvement of soybean oil quality. In soybean, the genes encoding these enzymes have not been completely described; there-fore, this study aimed to identify, characterize, and analyze the in silico expression of these genes in soybean. We identified two gene models encoding CPT and two gene models encoding LPCAT, one of which presented an alternative transcript. The sequences were positioned on the physical map of soybean and the promoter regions were analyzed. Cis-elements responsible for seed-specific expression and responses to biotic and abiotic stresses were identified. Virtual expression analysis of the gene models for CPT and LPCAT indicated that these genes are expressed under different stress conditions, in somatic embryos during differentiation, in immature seeds, root tissues, and calli. Putative ami-no acid sequences revealed the presence of transmembrane domains, and analysis of the cellular localization of these enzymes revealed they are located in the endoplasmic reticulum.engGenetics and Molecular Research15(3), gmr.15038974, Aug. 2016Lipid metabolism enzymesGene modelsCis-element identificationVirtual expressionPutative amino acid sequencesCellular localizationIn silico characterization of 1,2-diacylglycerol cholinephosphotransferase and lysophospha-tidylcholine acyltransferase genes in Glycine max L. 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dc.title.en.fl_str_mv |
In silico characterization of 1,2-diacylglycerol cholinephosphotransferase and lysophospha-tidylcholine acyltransferase genes in Glycine max L. Merrill. |
title |
In silico characterization of 1,2-diacylglycerol cholinephosphotransferase and lysophospha-tidylcholine acyltransferase genes in Glycine max L. Merrill. |
spellingShingle |
In silico characterization of 1,2-diacylglycerol cholinephosphotransferase and lysophospha-tidylcholine acyltransferase genes in Glycine max L. Merrill. Sousa, C.S. Lipid metabolism enzymes Gene models Cis-element identification Virtual expression Putative amino acid sequences Cellular localization |
title_short |
In silico characterization of 1,2-diacylglycerol cholinephosphotransferase and lysophospha-tidylcholine acyltransferase genes in Glycine max L. Merrill. |
title_full |
In silico characterization of 1,2-diacylglycerol cholinephosphotransferase and lysophospha-tidylcholine acyltransferase genes in Glycine max L. Merrill. |
title_fullStr |
In silico characterization of 1,2-diacylglycerol cholinephosphotransferase and lysophospha-tidylcholine acyltransferase genes in Glycine max L. Merrill. |
title_full_unstemmed |
In silico characterization of 1,2-diacylglycerol cholinephosphotransferase and lysophospha-tidylcholine acyltransferase genes in Glycine max L. Merrill. |
title_sort |
In silico characterization of 1,2-diacylglycerol cholinephosphotransferase and lysophospha-tidylcholine acyltransferase genes in Glycine max L. Merrill. |
author |
Sousa, C.S. |
author_facet |
Sousa, C.S. Barros, E.G. Moreira, M.A. Barros, B.A. Barh, D. Ghosh, P. Azevedo, V. |
author_role |
author |
author2 |
Barros, E.G. Moreira, M.A. Barros, B.A. Barh, D. Ghosh, P. Azevedo, V. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Sousa, C.S. Barros, E.G. Moreira, M.A. Barros, B.A. Barh, D. Ghosh, P. Azevedo, V. |
dc.subject.pt-BR.fl_str_mv |
Lipid metabolism enzymes Gene models Cis-element identification Virtual expression Putative amino acid sequences Cellular localization |
topic |
Lipid metabolism enzymes Gene models Cis-element identification Virtual expression Putative amino acid sequences Cellular localization |
description |
The enzymes 1,2-diacylglycerol cholinephosphotrans-ferase (CPT) and lysophosphatidylcholine acyltransferase (LPCAT) are important in lipid metabolism in soybean seeds. Thus, understand-ing the genes that encode these enzymes may enable their modification and aid the improvement of soybean oil quality. In soybean, the genes encoding these enzymes have not been completely described; there-fore, this study aimed to identify, characterize, and analyze the in silico expression of these genes in soybean. We identified two gene models encoding CPT and two gene models encoding LPCAT, one of which presented an alternative transcript. The sequences were positioned on the physical map of soybean and the promoter regions were analyzed. Cis-elements responsible for seed-specific expression and responses to biotic and abiotic stresses were identified. Virtual expression analysis of the gene models for CPT and LPCAT indicated that these genes are expressed under different stress conditions, in somatic embryos during differentiation, in immature seeds, root tissues, and calli. Putative ami-no acid sequences revealed the presence of transmembrane domains, and analysis of the cellular localization of these enzymes revealed they are located in the endoplasmic reticulum. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-08-26 |
dc.date.accessioned.fl_str_mv |
2017-10-31T09:36:03Z |
dc.date.available.fl_str_mv |
2017-10-31T09:36:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
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dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/gmr.15038974 http://www.locus.ufv.br/handle/123456789/12561 |
dc.identifier.issn.none.fl_str_mv |
16765680 |
identifier_str_mv |
16765680 |
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http://dx.doi.org/10.4238/gmr.15038974 http://www.locus.ufv.br/handle/123456789/12561 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
15(3), gmr.15038974, Aug. 2016 |
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Genetics and Molecular Research |
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Genetics and Molecular Research |
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