Resistência da soja à ferrugem-asiática: hibridação, herança e identificação de marcadores microssatélites
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/1345 |
Resumo: | Soybean is one of the main tillages in Brazil and Asian rust (ASR) is one of the main soybean‟s disease. Thus, the use of resistant cultivars is a strategy that can be used in the management of this disease in tillages. So, our objective was to analyze the reaction; estimate the number of evaluations needed to determine the difference among tested materials; study the genetic diversity of soybean cultivars in natural conditions of Asian soybean rust (ASR) infection; check the measurement similarity of several soybean characters obtained in two types of experimental parcels; determine the artificial hybridization efficiency between soybeans genitors and different reactions to ASR, considering the crossings times; estimate the heritability, the sorting gain and the average of improved population to characters related to the resistance and phenotypic characters, measured in F2 populations developed from crossings TMG 801 x BRS Valuable RR and TMG 803 x BRS Valuable RR; study F2 population segregation from crossing between TMG 803 and BRS Valuable RR to ASR and identify microsatellite markers related to the resistance gene. Three experiments were conducted at field, one in a greenhouse and other one in a laboratory. Cultivars used were: TMG 801, TMG 803, BRS Valuable RR, Aquamarine RR, UFVS 2010, NK 7059 RR and BRSGO 7560. The results indicated that cultivars TMG 801, TMG 803 and BRSGO 7560 showed resistance reaction to pathogen; four evaluations were sufficient to predict the degree of cultivars resistance; there is genetic variability among cultivars and potential genitors were identified for being used in breeding programs; the area under the rust curve at the most infected leaflet, the number of seeds, the seeds weight and the weight of 100 seeds showed similar results in the experimental parcels (Hill Plot and Row Plot Design); Artificial hybridizations performed, in January, from 10 a.m to 12 p.m., with average relative humidity of 34,1% and average temperature of 38,6°C, were the most efficient, followed by the period from 2 p.m. to 4 p.m., with average relative humidity of 30,7% and an average temperature of 41,7°C; the efficiency of artificial hybridizations was influenced by the difference between the genitors‟ maturity groups and the number of each genitors‟ plant; the heritability magnitude indicated possibility of selection gains and success in the identification of individuals with the lowest percentages of the most infected leaflet‟s infected area and the rust evolution in plant in precocious generations of breeding programs; the heritabilities estimation of days from planting to flowering, plant height at flowering, days from planting to maturity, plant height at maturity, number of pods per plant, number of seeds per plant and seeds weight were higher in crossing TMG 803 X BRS Valuable RR; the largest selection gains predicted were obtained in experiments without pathogen control with fungicides, in crossings TMG 801 x BRS Valuable RR and TMG 803 x BRS Valuable RR; the analysis, in group, of segregation and mapping indicated that the cultivar resistance TMG 803 to ASR is leaded by a gene with complete dominance; the resistance gene in the cultivar TMG 803 was mapped in Rpp4 resistance locus, in the linkage group G, and microsatellite marker sc21_3420 can be indicated for assisted selection of soybean genotypes aiming the introgression of TMG 803 resistance. |
id |
UFV_ed8e3e43bcc5f2ac0ea07e1b8ef4736d |
---|---|
oai_identifier_str |
oai:locus.ufv.br:123456789/1345 |
network_acronym_str |
UFV |
network_name_str |
LOCUS Repositório Institucional da UFV |
repository_id_str |
2145 |
spelling |
Matsuo, éderhttp://lattes.cnpq.br/1972912533706432Cruz, Cosme Damiãohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788274A6Brommonschenkel, Sérgio Hermíniohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780948Y4Sediyama, Tuneohttp://lattes.cnpq.br/4911178878735418Sediyama, Tociohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780090Y4Vieira, Rogério Fariahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780045J02015-03-26T12:45:31Z2012-11-272015-03-26T12:45:31Z2012-02-14MATSUO, éder. Development of soybean lineages resistant to rust assisted by molecular markers. 2012. 136 f. Tese (Doutorado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2012.http://locus.ufv.br/handle/123456789/1345Soybean is one of the main tillages in Brazil and Asian rust (ASR) is one of the main soybean‟s disease. Thus, the use of resistant cultivars is a strategy that can be used in the management of this disease in tillages. So, our objective was to analyze the reaction; estimate the number of evaluations needed to determine the difference among tested materials; study the genetic diversity of soybean cultivars in natural conditions of Asian soybean rust (ASR) infection; check the measurement similarity of several soybean characters obtained in two types of experimental parcels; determine the artificial hybridization efficiency between soybeans genitors and different reactions to ASR, considering the crossings times; estimate the heritability, the sorting gain and the average of improved population to characters related to the resistance and phenotypic characters, measured in F2 populations developed from crossings TMG 801 x BRS Valuable RR and TMG 803 x BRS Valuable RR; study F2 population segregation from crossing between TMG 803 and BRS Valuable RR to ASR and identify microsatellite markers related to the resistance gene. Three experiments were conducted at field, one in a greenhouse and other one in a laboratory. Cultivars used were: TMG 801, TMG 803, BRS Valuable RR, Aquamarine RR, UFVS 2010, NK 7059 RR and BRSGO 7560. The results indicated that cultivars TMG 801, TMG 803 and BRSGO 7560 showed resistance reaction to pathogen; four evaluations were sufficient to predict the degree of cultivars resistance; there is genetic variability among cultivars and potential genitors were identified for being used in breeding programs; the area under the rust curve at the most infected leaflet, the number of seeds, the seeds weight and the weight of 100 seeds showed similar results in the experimental parcels (Hill Plot and Row Plot Design); Artificial hybridizations performed, in January, from 10 a.m to 12 p.m., with average relative humidity of 34,1% and average temperature of 38,6°C, were the most efficient, followed by the period from 2 p.m. to 4 p.m., with average relative humidity of 30,7% and an average temperature of 41,7°C; the efficiency of artificial hybridizations was influenced by the difference between the genitors‟ maturity groups and the number of each genitors‟ plant; the heritability magnitude indicated possibility of selection gains and success in the identification of individuals with the lowest percentages of the most infected leaflet‟s infected area and the rust evolution in plant in precocious generations of breeding programs; the heritabilities estimation of days from planting to flowering, plant height at flowering, days from planting to maturity, plant height at maturity, number of pods per plant, number of seeds per plant and seeds weight were higher in crossing TMG 803 X BRS Valuable RR; the largest selection gains predicted were obtained in experiments without pathogen control with fungicides, in crossings TMG 801 x BRS Valuable RR and TMG 803 x BRS Valuable RR; the analysis, in group, of segregation and mapping indicated that the cultivar resistance TMG 803 to ASR is leaded by a gene with complete dominance; the resistance gene in the cultivar TMG 803 was mapped in Rpp4 resistance locus, in the linkage group G, and microsatellite marker sc21_3420 can be indicated for assisted selection of soybean genotypes aiming the introgression of TMG 803 resistance.A soja é uma das principais culturas do Brasil e a ferrugem asiática (FAS) é uma das principais doenças da cultura da soja. Logo, o uso de cultivares resistentes é um estratégia que pode ser utilizada no manejo dessa doença nas lavouras. Assim, objetivou-se analisar a reação; estimar o número de avaliações necessárias para determinar a diferença entre os materiais avaliados; estudar a diversidade genética de cultivares de soja em condições naturais de infecção da FAS; verificar a semelhança da mensuração de diversos caracteres da soja obtidos em dois tipos de parcelas experimentais; determinar a eficiência da hibridação artificial entre genitores de soja e diferentes reações à FAS, considerando os horários de cruzamentos; estimar a herdabilidade, o ganho por seleção e a média da população melhorada para caracteres relacionados com a resistência e caracteres fenotípicos, avaliados em populações F2 desenvolvidas a partir do cruzamento TMG 801 x BRS Valiosa RR e TMG 803 x BRS Valiosa RR; estudar a segregação da população F2, do cruzamento entre TMG 803 e BRS Valiosa RR, à FAS e identificar marcadores microssatélites ligados ao gene de resistência. Foram conduzidos três experimentos em campo, um em casa de vegetação e um em laboratório. As cultivares utilizadas foram: TMG 801, TMG 803, BRS Valiosa RR, Água-Marinha RR, UFVS 2010, NK 7059 RR e BRSGO 7560. Os resultados indicaram que as cultivares TMG 801, TMG 803 e BRSGO 7560 apresentaram reação de resistência ao patógeno; quatro avaliações foram suficientes para predizer o grau de resistência das cultivares; existe variabilidade genética entre as cultivares e foram identificados genitores potenciais para serem utilizados em programas de melhoramento; a área abaixo da curva da ferrugem no folíolo mais infectado, o número de sementes, o peso de sementes e o peso de 100 sementes apresentaram resultados semelhantes nas parcelas experimentais (Hill Plot Design e Row Plot Design); as hibridações artificiais realizadas, em janeiro, no período das 10:00h às 12:00h, com umidade relativa média de 34,1% e temperatura média de 38,6ºC, foram as mais eficientes, seguidas do período das 14:00h às 16:00h, com umidade relativa média de 30,7% e temperatura média de 41,7ºC; a eficiência das hibridações artificiais foi influenciada pela diferença entre grupos de maturidade dos genitores e número de plantas de cada genitor; a magnitude da herdabilidade indicou possibilidade de ganhos por seleção e sucesso na identificação de indivíduos com menores porcentagens da área infectada do folíolo mais infectado e da evolução da ferrugem na planta nas gerações precoces dos programas de melhoramento; as estimativas de herdabilidade de dias do plantio ao florescimento, altura de planta no florescimento, dias do plantio à maturação, altura de planta na maturação, número de vagens por planta, número de sementes por planta e peso de sementes foram maiores no cruzamento TMG 803 x BRS Valiosa RR; os maiores ganhos por seleção preditos foram obtidos nos experimentos sem o controle do patógeno com fungicida, nos cruzamentos TMG 801 x BRS Valiosa RR e TMG 803 x BRS Valiosa RR; a análise, em conjunto, da segregação e do mapeamento indicou que a resistência da cultivar TMG 803 à ferrugem asiática da soja é governada por um gene com dominância completa; o gene de resistência presente na cultivar TMG 803 foi mapeado no loco de resistência Rpp4, no grupo de ligação G, e o marcador microssatélite sc21_3420 pode ser indicado para seleção assistida de genótipos de soja visando a introgressão da resistência da TMG 803.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaDoutorado em Genética e MelhoramentoUFVBRGenética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; MeMelhoramento genéticoGlycine maxPhakopsora pachyrhiziMarcadores molecularesBreedingGlycine maxPhakopsora pachyrhiziMolecular markersCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETALResistência da soja à ferrugem-asiática: hibridação, herança e identificação de marcadores microssatélitesDevelopment of soybean lineages resistant to rust assisted by molecular markersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf2587898https://locus.ufv.br//bitstream/123456789/1345/1/texto%20completo.pdfc0ce62c90fab313d1c289c871ec41c72MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain295641https://locus.ufv.br//bitstream/123456789/1345/2/texto%20completo.pdf.txtcfc1a583125ebe09e882313f3d5f11e8MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3474https://locus.ufv.br//bitstream/123456789/1345/3/texto%20completo.pdf.jpg58625c9c3eec38191418638bae4bb77fMD53123456789/13452016-04-07 23:06:40.128oai:locus.ufv.br:123456789/1345Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-08T02:06:40LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Resistência da soja à ferrugem-asiática: hibridação, herança e identificação de marcadores microssatélites |
dc.title.alternative.eng.fl_str_mv |
Development of soybean lineages resistant to rust assisted by molecular markers |
title |
Resistência da soja à ferrugem-asiática: hibridação, herança e identificação de marcadores microssatélites |
spellingShingle |
Resistência da soja à ferrugem-asiática: hibridação, herança e identificação de marcadores microssatélites Matsuo, éder Melhoramento genético Glycine max Phakopsora pachyrhizi Marcadores moleculares Breeding Glycine max Phakopsora pachyrhizi Molecular markers CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL |
title_short |
Resistência da soja à ferrugem-asiática: hibridação, herança e identificação de marcadores microssatélites |
title_full |
Resistência da soja à ferrugem-asiática: hibridação, herança e identificação de marcadores microssatélites |
title_fullStr |
Resistência da soja à ferrugem-asiática: hibridação, herança e identificação de marcadores microssatélites |
title_full_unstemmed |
Resistência da soja à ferrugem-asiática: hibridação, herança e identificação de marcadores microssatélites |
title_sort |
Resistência da soja à ferrugem-asiática: hibridação, herança e identificação de marcadores microssatélites |
author |
Matsuo, éder |
author_facet |
Matsuo, éder |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/1972912533706432 |
dc.contributor.author.fl_str_mv |
Matsuo, éder |
dc.contributor.advisor-co1.fl_str_mv |
Cruz, Cosme Damião |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788274A6 |
dc.contributor.advisor-co2.fl_str_mv |
Brommonschenkel, Sérgio Hermínio |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780948Y4 |
dc.contributor.advisor1.fl_str_mv |
Sediyama, Tuneo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4911178878735418 |
dc.contributor.referee1.fl_str_mv |
Sediyama, Tocio |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780090Y4 |
dc.contributor.referee2.fl_str_mv |
Vieira, Rogério Faria |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780045J0 |
contributor_str_mv |
Cruz, Cosme Damião Brommonschenkel, Sérgio Hermínio Sediyama, Tuneo Sediyama, Tocio Vieira, Rogério Faria |
dc.subject.por.fl_str_mv |
Melhoramento genético Glycine max Phakopsora pachyrhizi Marcadores moleculares |
topic |
Melhoramento genético Glycine max Phakopsora pachyrhizi Marcadores moleculares Breeding Glycine max Phakopsora pachyrhizi Molecular markers CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL |
dc.subject.eng.fl_str_mv |
Breeding Glycine max Phakopsora pachyrhizi Molecular markers |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL |
description |
Soybean is one of the main tillages in Brazil and Asian rust (ASR) is one of the main soybean‟s disease. Thus, the use of resistant cultivars is a strategy that can be used in the management of this disease in tillages. So, our objective was to analyze the reaction; estimate the number of evaluations needed to determine the difference among tested materials; study the genetic diversity of soybean cultivars in natural conditions of Asian soybean rust (ASR) infection; check the measurement similarity of several soybean characters obtained in two types of experimental parcels; determine the artificial hybridization efficiency between soybeans genitors and different reactions to ASR, considering the crossings times; estimate the heritability, the sorting gain and the average of improved population to characters related to the resistance and phenotypic characters, measured in F2 populations developed from crossings TMG 801 x BRS Valuable RR and TMG 803 x BRS Valuable RR; study F2 population segregation from crossing between TMG 803 and BRS Valuable RR to ASR and identify microsatellite markers related to the resistance gene. Three experiments were conducted at field, one in a greenhouse and other one in a laboratory. Cultivars used were: TMG 801, TMG 803, BRS Valuable RR, Aquamarine RR, UFVS 2010, NK 7059 RR and BRSGO 7560. The results indicated that cultivars TMG 801, TMG 803 and BRSGO 7560 showed resistance reaction to pathogen; four evaluations were sufficient to predict the degree of cultivars resistance; there is genetic variability among cultivars and potential genitors were identified for being used in breeding programs; the area under the rust curve at the most infected leaflet, the number of seeds, the seeds weight and the weight of 100 seeds showed similar results in the experimental parcels (Hill Plot and Row Plot Design); Artificial hybridizations performed, in January, from 10 a.m to 12 p.m., with average relative humidity of 34,1% and average temperature of 38,6°C, were the most efficient, followed by the period from 2 p.m. to 4 p.m., with average relative humidity of 30,7% and an average temperature of 41,7°C; the efficiency of artificial hybridizations was influenced by the difference between the genitors‟ maturity groups and the number of each genitors‟ plant; the heritability magnitude indicated possibility of selection gains and success in the identification of individuals with the lowest percentages of the most infected leaflet‟s infected area and the rust evolution in plant in precocious generations of breeding programs; the heritabilities estimation of days from planting to flowering, plant height at flowering, days from planting to maturity, plant height at maturity, number of pods per plant, number of seeds per plant and seeds weight were higher in crossing TMG 803 X BRS Valuable RR; the largest selection gains predicted were obtained in experiments without pathogen control with fungicides, in crossings TMG 801 x BRS Valuable RR and TMG 803 x BRS Valuable RR; the analysis, in group, of segregation and mapping indicated that the cultivar resistance TMG 803 to ASR is leaded by a gene with complete dominance; the resistance gene in the cultivar TMG 803 was mapped in Rpp4 resistance locus, in the linkage group G, and microsatellite marker sc21_3420 can be indicated for assisted selection of soybean genotypes aiming the introgression of TMG 803 resistance. |
publishDate |
2012 |
dc.date.available.fl_str_mv |
2012-11-27 2015-03-26T12:45:31Z |
dc.date.issued.fl_str_mv |
2012-02-14 |
dc.date.accessioned.fl_str_mv |
2015-03-26T12:45:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MATSUO, éder. Development of soybean lineages resistant to rust assisted by molecular markers. 2012. 136 f. Tese (Doutorado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2012. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/1345 |
identifier_str_mv |
MATSUO, éder. Development of soybean lineages resistant to rust assisted by molecular markers. 2012. 136 f. Tese (Doutorado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2012. |
url |
http://locus.ufv.br/handle/123456789/1345 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.publisher.program.fl_str_mv |
Doutorado em Genética e Melhoramento |
dc.publisher.initials.fl_str_mv |
UFV |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me |
publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
bitstream.url.fl_str_mv |
https://locus.ufv.br//bitstream/123456789/1345/1/texto%20completo.pdf https://locus.ufv.br//bitstream/123456789/1345/2/texto%20completo.pdf.txt https://locus.ufv.br//bitstream/123456789/1345/3/texto%20completo.pdf.jpg |
bitstream.checksum.fl_str_mv |
c0ce62c90fab313d1c289c871ec41c72 cfc1a583125ebe09e882313f3d5f11e8 58625c9c3eec38191418638bae4bb77f |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1798053345984774144 |