Production of Brazilian human norovirus VLPs and comparison of purification methods
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UnB |
Texto Completo: | http://repositorio.unb.br/handle/10482/29849 http://dx.doi.org/10.1590/S1517-838246420140925 |
Resumo: | Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation. |
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Lamounier, Thaís Alves da CostaOliveira, Layssa Miranda deCamargo, Brenda Rabello deRodrigues, Kelly BarretoNoronha, Eliane FerreiraRibeiro, Bergmann MoraisNagata, Tatsuya2017-12-07T05:13:27Z2017-12-07T05:13:27Z2015-10LAMOUNIER, Thais Alves da Costa et al. Production of Brazilian human norovirus VLPs and comparison of purification methods. Brazilian Journal of Microbiology, São Paulo, v. 46, n. 4, p. 1265-1268, out./dez. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265&lng=en&nrm=iso>. Acesso em: 2 abr. 2018. Epub Oct 09, 2015. doi: http://dx.doi.org/10.1590/S1517-838246420140925.http://repositorio.unb.br/handle/10482/29849http://dx.doi.org/10.1590/S1517-838246420140925Sociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology - All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC-BY (CC BY NC 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265&lng=en&nrm=iso. Acesso em: 2 abr. 2018.info:eu-repo/semantics/openAccessProduction of Brazilian human norovirus VLPs and comparison of purification methodsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleVírusCromatografia de troca iônicaNoroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.engreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALARTIGO_ProductionBrazilianHuman.pdfARTIGO_ProductionBrazilianHuman.pdfapplication/pdf585503http://repositorio2.unb.br/jspui/bitstream/10482/29849/2/ARTIGO_ProductionBrazilianHuman.pdfc6ba1ad3fbd4ba6341207dc1583b87c5MD52open access10482/298492023-05-24 20:34:34.538open accessoai:repositorio2.unb.br:10482/29849Biblioteca Digital de Teses e DissertaçõesPUBhttps://repositorio.unb.br/oai/requestopendoar:2023-05-24T23:34:34Repositório Institucional da UnB - Universidade de Brasília (UnB)false |
dc.title.pt_BR.fl_str_mv |
Production of Brazilian human norovirus VLPs and comparison of purification methods |
title |
Production of Brazilian human norovirus VLPs and comparison of purification methods |
spellingShingle |
Production of Brazilian human norovirus VLPs and comparison of purification methods Lamounier, Thaís Alves da Costa Vírus Cromatografia de troca iônica |
title_short |
Production of Brazilian human norovirus VLPs and comparison of purification methods |
title_full |
Production of Brazilian human norovirus VLPs and comparison of purification methods |
title_fullStr |
Production of Brazilian human norovirus VLPs and comparison of purification methods |
title_full_unstemmed |
Production of Brazilian human norovirus VLPs and comparison of purification methods |
title_sort |
Production of Brazilian human norovirus VLPs and comparison of purification methods |
author |
Lamounier, Thaís Alves da Costa |
author_facet |
Lamounier, Thaís Alves da Costa Oliveira, Layssa Miranda de Camargo, Brenda Rabello de Rodrigues, Kelly Barreto Noronha, Eliane Ferreira Ribeiro, Bergmann Morais Nagata, Tatsuya |
author_role |
author |
author2 |
Oliveira, Layssa Miranda de Camargo, Brenda Rabello de Rodrigues, Kelly Barreto Noronha, Eliane Ferreira Ribeiro, Bergmann Morais Nagata, Tatsuya |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Lamounier, Thaís Alves da Costa Oliveira, Layssa Miranda de Camargo, Brenda Rabello de Rodrigues, Kelly Barreto Noronha, Eliane Ferreira Ribeiro, Bergmann Morais Nagata, Tatsuya |
dc.subject.keyword.pt_BR.fl_str_mv |
Vírus Cromatografia de troca iônica |
topic |
Vírus Cromatografia de troca iônica |
description |
Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-10 |
dc.date.accessioned.fl_str_mv |
2017-12-07T05:13:27Z |
dc.date.available.fl_str_mv |
2017-12-07T05:13:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LAMOUNIER, Thais Alves da Costa et al. Production of Brazilian human norovirus VLPs and comparison of purification methods. Brazilian Journal of Microbiology, São Paulo, v. 46, n. 4, p. 1265-1268, out./dez. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265&lng=en&nrm=iso>. Acesso em: 2 abr. 2018. Epub Oct 09, 2015. doi: http://dx.doi.org/10.1590/S1517-838246420140925. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unb.br/handle/10482/29849 |
dc.identifier.doi.pt_BR.fl_str_mv |
http://dx.doi.org/10.1590/S1517-838246420140925 |
identifier_str_mv |
LAMOUNIER, Thais Alves da Costa et al. Production of Brazilian human norovirus VLPs and comparison of purification methods. Brazilian Journal of Microbiology, São Paulo, v. 46, n. 4, p. 1265-1268, out./dez. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265&lng=en&nrm=iso>. Acesso em: 2 abr. 2018. Epub Oct 09, 2015. doi: http://dx.doi.org/10.1590/S1517-838246420140925. |
url |
http://repositorio.unb.br/handle/10482/29849 http://dx.doi.org/10.1590/S1517-838246420140925 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
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Universidade de Brasília (UnB) |
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UNB |
institution |
UNB |
reponame_str |
Repositório Institucional da UnB |
collection |
Repositório Institucional da UnB |
bitstream.url.fl_str_mv |
http://repositorio2.unb.br/jspui/bitstream/10482/29849/2/ARTIGO_ProductionBrazilianHuman.pdf |
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c6ba1ad3fbd4ba6341207dc1583b87c5 |
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Repositório Institucional da UnB - Universidade de Brasília (UnB) |
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