Isolation and Characterisation of a Reserve Protein from the Seeds of Cereus jamacaru (Cactaceae)

Detalhes bibliográficos
Autor(a) principal: Costa, Itayguara Ribeiro da
Data de Publicação: 2001
Outros Autores: Souza, Petrônio Augusto Simão de, Bloch Jr., Carlos, Llamoca-Zárate, Romulo Marino, Campos, Francisco A. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/25815
https://dx.doi.org/10.1590/S1516-89132001000400002
Resumo: We describe here the isolation and characterisation of a major reserve protein from the seeds of Cereus jamacaru. (Cactaceae). This protein has a molecular mass of 5319 kDa and was isolated by a combination of gel filtration chromatography and reverse phase HPLC. The amino acid composition of the protein was determined and it was shown to have similarities with the amino acid composition of several proteins from the 2S albumin storage protein family. The usefulness of this protein as a molecular marker in the Cactaceae is also discussed.
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spelling Costa, Itayguara Ribeiro daSouza, Petrônio Augusto Simão deBloch Jr., CarlosLlamoca-Zárate, Romulo MarinoCampos, Francisco A. P.2017-12-07T04:35:01Z2017-12-07T04:35:01Z2001Braz. arch. biol. technol.,v.44,n.4,p.331-335,2001http://repositorio.unb.br/handle/10482/25815https://dx.doi.org/10.1590/S1516-89132001000400002We describe here the isolation and characterisation of a major reserve protein from the seeds of Cereus jamacaru. (Cactaceae). This protein has a molecular mass of 5319 kDa and was isolated by a combination of gel filtration chromatography and reverse phase HPLC. The amino acid composition of the protein was determined and it was shown to have similarities with the amino acid composition of several proteins from the 2S albumin storage protein family. The usefulness of this protein as a molecular marker in the Cactaceae is also discussed.A proteína de reserva mais abundante das sementes de Cereus jamacaru (Cactaceae) foi isolada e caracterizada. Esta proteína tem uma massa molecular de 5319 kDa e foi isolada através de uma combinação de técnicas de filtração em gel e HPLC de fase reversa. A composição de aminoácidos da proteína foi determinada e possui similaridade com a composição de aminoácidos de diversas proteínas de reserva de sementes que pertencem à família das albuminas. A utilidade desta proteína como um marcador molecular para as cactáceas é também discutida.Em processamentoInstituto de Tecnologia do Paraná - TecparIsolation and Characterisation of a Reserve Protein from the Seeds of Cereus jamacaru (Cactaceae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCereus jamacarumandacarúCactaceaeseed proteins2S albuminsinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALa02v44n4.pdfapplication/pdf164728http://repositorio2.unb.br/jspui/bitstream/10482/25815/1/a02v44n4.pdf1a86c7af51247f0337c248e2390bb7bbMD51open access10482/258152023-10-11 14:29:40.155open accessoai:repositorio2.unb.br:10482/25815Biblioteca Digital de Teses e DissertaçõesPUBhttps://repositorio.unb.br/oai/requestopendoar:2023-10-11T17:29:40Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.pt_BR.fl_str_mv Isolation and Characterisation of a Reserve Protein from the Seeds of Cereus jamacaru (Cactaceae)
title Isolation and Characterisation of a Reserve Protein from the Seeds of Cereus jamacaru (Cactaceae)
spellingShingle Isolation and Characterisation of a Reserve Protein from the Seeds of Cereus jamacaru (Cactaceae)
Costa, Itayguara Ribeiro da
Cereus jamacaru
mandacarú
Cactaceae
seed proteins
2S albumins
title_short Isolation and Characterisation of a Reserve Protein from the Seeds of Cereus jamacaru (Cactaceae)
title_full Isolation and Characterisation of a Reserve Protein from the Seeds of Cereus jamacaru (Cactaceae)
title_fullStr Isolation and Characterisation of a Reserve Protein from the Seeds of Cereus jamacaru (Cactaceae)
title_full_unstemmed Isolation and Characterisation of a Reserve Protein from the Seeds of Cereus jamacaru (Cactaceae)
title_sort Isolation and Characterisation of a Reserve Protein from the Seeds of Cereus jamacaru (Cactaceae)
author Costa, Itayguara Ribeiro da
author_facet Costa, Itayguara Ribeiro da
Souza, Petrônio Augusto Simão de
Bloch Jr., Carlos
Llamoca-Zárate, Romulo Marino
Campos, Francisco A. P.
author_role author
author2 Souza, Petrônio Augusto Simão de
Bloch Jr., Carlos
Llamoca-Zárate, Romulo Marino
Campos, Francisco A. P.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Costa, Itayguara Ribeiro da
Souza, Petrônio Augusto Simão de
Bloch Jr., Carlos
Llamoca-Zárate, Romulo Marino
Campos, Francisco A. P.
dc.subject.keyword.pt_BR.fl_str_mv Cereus jamacaru
mandacarú
Cactaceae
seed proteins
2S albumins
topic Cereus jamacaru
mandacarú
Cactaceae
seed proteins
2S albumins
description We describe here the isolation and characterisation of a major reserve protein from the seeds of Cereus jamacaru. (Cactaceae). This protein has a molecular mass of 5319 kDa and was isolated by a combination of gel filtration chromatography and reverse phase HPLC. The amino acid composition of the protein was determined and it was shown to have similarities with the amino acid composition of several proteins from the 2S albumin storage protein family. The usefulness of this protein as a molecular marker in the Cactaceae is also discussed.
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2017-12-07T04:35:01Z
dc.date.available.fl_str_mv 2017-12-07T04:35:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Braz. arch. biol. technol.,v.44,n.4,p.331-335,2001
dc.identifier.uri.fl_str_mv http://repositorio.unb.br/handle/10482/25815
dc.identifier.doi.pt_BR.fl_str_mv https://dx.doi.org/10.1590/S1516-89132001000400002
identifier_str_mv Braz. arch. biol. technol.,v.44,n.4,p.331-335,2001
url http://repositorio.unb.br/handle/10482/25815
https://dx.doi.org/10.1590/S1516-89132001000400002
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
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repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
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