NLRP3 inflammasome activation increase IL1β levels and cause impairment cognitive in pneumococcal meningitis

Detalhes bibliográficos
Autor(a) principal: Junior, Silvio Renato Ribeiro
Data de Publicação: 2018
Outros Autores: Collodel, Allan, Faller, Cristiano Julio, Lodetti, Bruna França, Pizzol, Felipe Dal, Barichello, Tatiana
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UNESC
Texto Completo: http://repositorio.unesc.net/handle/1/6754
Resumo: Artigo apresentado como requisito parcial para obtenção do grau de Bacharel, no Curso de Medicina, da Universidade do Extremo Sul Catarinense- UNESC.
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spelling Junior, Silvio Renato RibeiroCollodel, AllanFaller, Cristiano JulioLodetti, Bruna FrançaPizzol, Felipe DalBarichello, TatianaGeneroso, Jaqueline da SilvaBudni, JosianeUniversidade do Extremo Sul Catarinense2019-04-10T13:28:10Z2019-04-10T13:28:10Z2018-12http://repositorio.unesc.net/handle/1/6754Artigo apresentado como requisito parcial para obtenção do grau de Bacharel, no Curso de Medicina, da Universidade do Extremo Sul Catarinense- UNESC.Pneumococcal meningitis is an important cause of morbidity and mortality worldwide, and the most common causative organism of community acquired bacterial meningitis in adults is Streptococcus pneumoniae. Studies have examined the role of inflammasomes in bacterial meningitis and demonstrated the NLR are also involved in the recognition of S. pneumoniae by the innate immune system. In this context, the aim of the present study was to evaluate the NLRP3 expression and IL1B levels in hippocamp and frontal cortex and behavioral parameters in adult Wistar rats submitted to pneumococcal meningitis. Where use male Wistar rats, the bacterial meningitis induction was performed under anaesthesia, the animals received intracisternal injection of 10 μL of artificial cerebrospinal fluid (CSF) as a placebo or an equivalent volume of S. pneumoniae suspension. At 18 hours we confirm the meningitis infection and the animals destined to the behavioral tests received antimicrobial treatment. For the 24 hours protocol the animals were divided into control (n = 6), meningitis (n = 6), after this time the hippocampus and prefrontal cortex were removed for evaluation of NLRP3 expression and levels of IL-1β. In the protocol for behavioral tests at 10 days, the animals were divided into control (n = 10), meningitis (n = 10). After the behavioral tests the animals were euthanized, and the hippocampal and prefrontal cortex structures removed for evaluation of NLRP3 expression and levels of IL-1β. The expression of NLRP3 was increased in both brain structures, of the meningitis group when compared to the control group. In the task of habituation to the open field, there were no differences in the groups in the training session. In the test session, there was a significant reduction in crosses and withdrawals in the control group demonstrating habituation memory in these groups. However, the meningitis group showed no difference between the training and test sessions, demonstrating impairment of habituation memory in this group. In the behavioral test of recognition of new objects, the animals of the meningitis group presented memory impairment of the object recognition, they did not use a significantly longer time exploring the new object, presenting loss of long-term memory. However, the animals in the control group had long-term memory between the test sessions compared to the training session. In the inhibitory avoidance task there was a difference between the training and test sessions in the control group and in the meningitis group there was no difference between the training and test sessions, demonstrating aversive memory impairment in these animals. NLRP3 expression at 10 days post-induction was increased in the hippocampus and prefrontal cortex of the meningitis group when compared to the control group. In conclusion, we showed that experimental meningitis model induced the NLRP3 inflamassome activation, increased the IL1B levels and impairment behaviours in rats.Inflamassoma nlrp3Meningite pneumocócicaNeuroinflamaçãoNLRP3 inflammasome activation increase IL1β levels and cause impairment cognitive in pneumococcal meningitisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisporreponame:Repositório Institucional da UNESCinstname:Universidade do Extremo Sul Catarinense (Unesc)instacron:UNESCinfo:eu-repo/semantics/openAccessORIGINALInformação do texto competo - Medicina.pdfInformação do texto competo - Medicina.pdfTCCapplication/pdf4584http://repositorio.unesc.net/bitstream/1/6754/1/Informa%c3%a7%c3%a3o%20do%20texto%20competo%20-%20Medicina.pdf1c6e9dc40d415f218ecfb092b602798eMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.unesc.net/bitstream/1/6754/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD521/67542019-11-14 17:58:13.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Repositório de Publicaçõeshttp://repositorio.unesc.net/
dc.title.pt_BR.fl_str_mv NLRP3 inflammasome activation increase IL1β levels and cause impairment cognitive in pneumococcal meningitis
title NLRP3 inflammasome activation increase IL1β levels and cause impairment cognitive in pneumococcal meningitis
spellingShingle NLRP3 inflammasome activation increase IL1β levels and cause impairment cognitive in pneumococcal meningitis
Junior, Silvio Renato Ribeiro
Inflamassoma nlrp3
Meningite pneumocócica
Neuroinflamação
title_short NLRP3 inflammasome activation increase IL1β levels and cause impairment cognitive in pneumococcal meningitis
title_full NLRP3 inflammasome activation increase IL1β levels and cause impairment cognitive in pneumococcal meningitis
title_fullStr NLRP3 inflammasome activation increase IL1β levels and cause impairment cognitive in pneumococcal meningitis
title_full_unstemmed NLRP3 inflammasome activation increase IL1β levels and cause impairment cognitive in pneumococcal meningitis
title_sort NLRP3 inflammasome activation increase IL1β levels and cause impairment cognitive in pneumococcal meningitis
author Junior, Silvio Renato Ribeiro
author_facet Junior, Silvio Renato Ribeiro
Collodel, Allan
Faller, Cristiano Julio
Lodetti, Bruna França
Pizzol, Felipe Dal
Barichello, Tatiana
author_role author
author2 Collodel, Allan
Faller, Cristiano Julio
Lodetti, Bruna França
Pizzol, Felipe Dal
Barichello, Tatiana
author2_role author
author
author
author
author
dc.contributor.other.none.fl_str_mv Generoso, Jaqueline da Silva
dc.contributor.author.fl_str_mv Junior, Silvio Renato Ribeiro
Collodel, Allan
Faller, Cristiano Julio
Lodetti, Bruna França
Pizzol, Felipe Dal
Barichello, Tatiana
dc.contributor.advisor1.fl_str_mv Budni, Josiane
contributor_str_mv Budni, Josiane
dc.subject.por.fl_str_mv Inflamassoma nlrp3
Meningite pneumocócica
Neuroinflamação
topic Inflamassoma nlrp3
Meningite pneumocócica
Neuroinflamação
dc.description.pt_BR.fl_txt_mv Artigo apresentado como requisito parcial para obtenção do grau de Bacharel, no Curso de Medicina, da Universidade do Extremo Sul Catarinense- UNESC.
dc.description.abstract.por.fl_txt_mv Pneumococcal meningitis is an important cause of morbidity and mortality worldwide, and the most common causative organism of community acquired bacterial meningitis in adults is Streptococcus pneumoniae. Studies have examined the role of inflammasomes in bacterial meningitis and demonstrated the NLR are also involved in the recognition of S. pneumoniae by the innate immune system. In this context, the aim of the present study was to evaluate the NLRP3 expression and IL1B levels in hippocamp and frontal cortex and behavioral parameters in adult Wistar rats submitted to pneumococcal meningitis. Where use male Wistar rats, the bacterial meningitis induction was performed under anaesthesia, the animals received intracisternal injection of 10 μL of artificial cerebrospinal fluid (CSF) as a placebo or an equivalent volume of S. pneumoniae suspension. At 18 hours we confirm the meningitis infection and the animals destined to the behavioral tests received antimicrobial treatment. For the 24 hours protocol the animals were divided into control (n = 6), meningitis (n = 6), after this time the hippocampus and prefrontal cortex were removed for evaluation of NLRP3 expression and levels of IL-1β. In the protocol for behavioral tests at 10 days, the animals were divided into control (n = 10), meningitis (n = 10). After the behavioral tests the animals were euthanized, and the hippocampal and prefrontal cortex structures removed for evaluation of NLRP3 expression and levels of IL-1β. The expression of NLRP3 was increased in both brain structures, of the meningitis group when compared to the control group. In the task of habituation to the open field, there were no differences in the groups in the training session. In the test session, there was a significant reduction in crosses and withdrawals in the control group demonstrating habituation memory in these groups. However, the meningitis group showed no difference between the training and test sessions, demonstrating impairment of habituation memory in this group. In the behavioral test of recognition of new objects, the animals of the meningitis group presented memory impairment of the object recognition, they did not use a significantly longer time exploring the new object, presenting loss of long-term memory. However, the animals in the control group had long-term memory between the test sessions compared to the training session. In the inhibitory avoidance task there was a difference between the training and test sessions in the control group and in the meningitis group there was no difference between the training and test sessions, demonstrating aversive memory impairment in these animals. NLRP3 expression at 10 days post-induction was increased in the hippocampus and prefrontal cortex of the meningitis group when compared to the control group. In conclusion, we showed that experimental meningitis model induced the NLRP3 inflamassome activation, increased the IL1B levels and impairment behaviours in rats.
description Artigo apresentado como requisito parcial para obtenção do grau de Bacharel, no Curso de Medicina, da Universidade do Extremo Sul Catarinense- UNESC.
publishDate 2018
dc.date.created.fl_str_mv 2018-12
dc.date.accessioned.fl_str_mv 2019-04-10T13:28:10Z
dc.date.available.fl_str_mv 2019-04-10T13:28:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
status_str publishedVersion
format masterThesis
dc.identifier.uri.fl_str_mv http://repositorio.unesc.net/handle/1/6754
url http://repositorio.unesc.net/handle/1/6754
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language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.coverage.spatial.pt_BR.fl_str_mv Universidade do Extremo Sul Catarinense
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNESC
instname:Universidade do Extremo Sul Catarinense (Unesc)
instacron:UNESC
instname_str Universidade do Extremo Sul Catarinense (Unesc)
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reponame_str Repositório Institucional da UNESC
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