Culture and molecular analysis of Enterococcus faecalis and antimicrobial susceptibility of clinical isolates from patients with failure endodontic treatment
Main Author: | |
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Publication Date: | 2014 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Brazilian Dental Science |
Download full: | https://ojs.ict.unesp.br/index.php/cob/article/view/1016 |
Summary: | Objective: The aim of this study was to evaluate the minimal inhibitory concentrations (MIC) of different antibiotic agents against to the most prevalent microorganism found in root-filled canals by culture and molecular approaches. Material and Methods: The microbial samples were taken either from thirty root-filled canals after removal of gutta-percha. Culture methods and 16s rDNA assay were used to identify the E faecails present in the samples. The antimicrobial susceptibilities of the isolates of E faecalis were determined by MIC values using the E test System and interpreted according to the Clinical and Laboratory Standards Institute guidelines. The following antibiotics were used: benzylpenicillin, amoxicillin, amoxicillin-clavulanic acid, erythromycin, azithromycin, vancomycin, chloramphenicol, tetracycline, doxycycline, ciprofloxacin, rifampicin and moxifloxacin. Results: E faecalis were isolated (7/30) and detected (13/30) by culture and PCR assay, respectively. All tested E faecalis (n=12) were highly sensitive to amoxicillin, moxifloxacin, vancomycin, benzylpenicillin and amoxicillin-clavulanic acid. Some antibiotics were resistant against E faecalis strains such as rifampicin (4/12), tetracycline (2/12), doxycycline (1/12), erythromycin (3/12) and azythromycin (8/12). Conclusion: Amoxicillin, amoxicillin-clavulanic acid, benzylpenicillin, vancomycin and moxifloxacin were the most active antibiotics, in vitro, against E faecalis clinical strains, with all the isolates being susceptible. Azithromycin and erythromycin were least effective, with none percentage of isolates being susceptible, during laboratory testing. Moreover, E faecalis were identified more frequently by PCR assay than by culture technique.Keywords: Retreatment; Antibiotics, antimicrobial susceptibility; Enterococcus faecalis; Antibiotic resistance. |
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oai:ojs.pkp.sfu.ca:article/1016 |
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network_name_str |
Brazilian Dental Science |
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Culture and molecular analysis of Enterococcus faecalis and antimicrobial susceptibility of clinical isolates from patients with failure endodontic treatmentObjective: The aim of this study was to evaluate the minimal inhibitory concentrations (MIC) of different antibiotic agents against to the most prevalent microorganism found in root-filled canals by culture and molecular approaches. Material and Methods: The microbial samples were taken either from thirty root-filled canals after removal of gutta-percha. Culture methods and 16s rDNA assay were used to identify the E faecails present in the samples. The antimicrobial susceptibilities of the isolates of E faecalis were determined by MIC values using the E test System and interpreted according to the Clinical and Laboratory Standards Institute guidelines. The following antibiotics were used: benzylpenicillin, amoxicillin, amoxicillin-clavulanic acid, erythromycin, azithromycin, vancomycin, chloramphenicol, tetracycline, doxycycline, ciprofloxacin, rifampicin and moxifloxacin. Results: E faecalis were isolated (7/30) and detected (13/30) by culture and PCR assay, respectively. All tested E faecalis (n=12) were highly sensitive to amoxicillin, moxifloxacin, vancomycin, benzylpenicillin and amoxicillin-clavulanic acid. Some antibiotics were resistant against E faecalis strains such as rifampicin (4/12), tetracycline (2/12), doxycycline (1/12), erythromycin (3/12) and azythromycin (8/12). Conclusion: Amoxicillin, amoxicillin-clavulanic acid, benzylpenicillin, vancomycin and moxifloxacin were the most active antibiotics, in vitro, against E faecalis clinical strains, with all the isolates being susceptible. Azithromycin and erythromycin were least effective, with none percentage of isolates being susceptible, during laboratory testing. Moreover, E faecalis were identified more frequently by PCR assay than by culture technique.Keywords: Retreatment; Antibiotics, antimicrobial susceptibility; Enterococcus faecalis; Antibiotic resistance. Institute of Science and Technology of São José dos Campos2014-09-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.ict.unesp.br/index.php/cob/article/view/101610.14295/bds.2014.v17i3.1016Brazilian Dental Science; Vol. 17 No. 3 (2014): Jul. - Sep. / 2014 - Published August 2014; 83-91Brazilian Dental Science; v. 17 n. 3 (2014): Jul. - Sep. / 2014 - Published August 2014; 83-912178-6011reponame:Brazilian Dental Scienceinstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESPenghttps://ojs.ict.unesp.br/index.php/cob/article/view/1016/885Endo, Marcos SergioSignoretti, Fernanda Graziela CorrêaKitayama, Vivian SayuriMarinho, Ariane Cássia SalustianoMartinho, Frederico CanatoGomes, Brenda Paula Figueiredo de Almeidainfo:eu-repo/semantics/openAccess2020-01-28T12:31:07Zoai:ojs.pkp.sfu.ca:article/1016Revistahttp://bds.ict.unesp.br/PUBhttp://ojs.fosjc.unesp.br/index.php/index/oaisergio@fosjc.unesp.br||sergio@fosjc.unesp.br2178-60112178-6011opendoar:2022-11-08T16:29:58.173773Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)true |
dc.title.none.fl_str_mv |
Culture and molecular analysis of Enterococcus faecalis and antimicrobial susceptibility of clinical isolates from patients with failure endodontic treatment |
title |
Culture and molecular analysis of Enterococcus faecalis and antimicrobial susceptibility of clinical isolates from patients with failure endodontic treatment |
spellingShingle |
Culture and molecular analysis of Enterococcus faecalis and antimicrobial susceptibility of clinical isolates from patients with failure endodontic treatment Endo, Marcos Sergio |
title_short |
Culture and molecular analysis of Enterococcus faecalis and antimicrobial susceptibility of clinical isolates from patients with failure endodontic treatment |
title_full |
Culture and molecular analysis of Enterococcus faecalis and antimicrobial susceptibility of clinical isolates from patients with failure endodontic treatment |
title_fullStr |
Culture and molecular analysis of Enterococcus faecalis and antimicrobial susceptibility of clinical isolates from patients with failure endodontic treatment |
title_full_unstemmed |
Culture and molecular analysis of Enterococcus faecalis and antimicrobial susceptibility of clinical isolates from patients with failure endodontic treatment |
title_sort |
Culture and molecular analysis of Enterococcus faecalis and antimicrobial susceptibility of clinical isolates from patients with failure endodontic treatment |
author |
Endo, Marcos Sergio |
author_facet |
Endo, Marcos Sergio Signoretti, Fernanda Graziela Corrêa Kitayama, Vivian Sayuri Marinho, Ariane Cássia Salustiano Martinho, Frederico Canato Gomes, Brenda Paula Figueiredo de Almeida |
author_role |
author |
author2 |
Signoretti, Fernanda Graziela Corrêa Kitayama, Vivian Sayuri Marinho, Ariane Cássia Salustiano Martinho, Frederico Canato Gomes, Brenda Paula Figueiredo de Almeida |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Endo, Marcos Sergio Signoretti, Fernanda Graziela Corrêa Kitayama, Vivian Sayuri Marinho, Ariane Cássia Salustiano Martinho, Frederico Canato Gomes, Brenda Paula Figueiredo de Almeida |
description |
Objective: The aim of this study was to evaluate the minimal inhibitory concentrations (MIC) of different antibiotic agents against to the most prevalent microorganism found in root-filled canals by culture and molecular approaches. Material and Methods: The microbial samples were taken either from thirty root-filled canals after removal of gutta-percha. Culture methods and 16s rDNA assay were used to identify the E faecails present in the samples. The antimicrobial susceptibilities of the isolates of E faecalis were determined by MIC values using the E test System and interpreted according to the Clinical and Laboratory Standards Institute guidelines. The following antibiotics were used: benzylpenicillin, amoxicillin, amoxicillin-clavulanic acid, erythromycin, azithromycin, vancomycin, chloramphenicol, tetracycline, doxycycline, ciprofloxacin, rifampicin and moxifloxacin. Results: E faecalis were isolated (7/30) and detected (13/30) by culture and PCR assay, respectively. All tested E faecalis (n=12) were highly sensitive to amoxicillin, moxifloxacin, vancomycin, benzylpenicillin and amoxicillin-clavulanic acid. Some antibiotics were resistant against E faecalis strains such as rifampicin (4/12), tetracycline (2/12), doxycycline (1/12), erythromycin (3/12) and azythromycin (8/12). Conclusion: Amoxicillin, amoxicillin-clavulanic acid, benzylpenicillin, vancomycin and moxifloxacin were the most active antibiotics, in vitro, against E faecalis clinical strains, with all the isolates being susceptible. Azithromycin and erythromycin were least effective, with none percentage of isolates being susceptible, during laboratory testing. Moreover, E faecalis were identified more frequently by PCR assay than by culture technique.Keywords: Retreatment; Antibiotics, antimicrobial susceptibility; Enterococcus faecalis; Antibiotic resistance. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/1016 10.14295/bds.2014.v17i3.1016 |
url |
https://ojs.ict.unesp.br/index.php/cob/article/view/1016 |
identifier_str_mv |
10.14295/bds.2014.v17i3.1016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/1016/885 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
dc.source.none.fl_str_mv |
Brazilian Dental Science; Vol. 17 No. 3 (2014): Jul. - Sep. / 2014 - Published August 2014; 83-91 Brazilian Dental Science; v. 17 n. 3 (2014): Jul. - Sep. / 2014 - Published August 2014; 83-91 2178-6011 reponame:Brazilian Dental Science instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Brazilian Dental Science |
collection |
Brazilian Dental Science |
repository.name.fl_str_mv |
Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
repository.mail.fl_str_mv |
sergio@fosjc.unesp.br||sergio@fosjc.unesp.br |
_version_ |
1788346898995740672 |