Total protein level reduction of odontopathogens biofilms by Rosmarinus officinalis L. (rosemary) extract: an analysis on Candida albicans and Streptococcus mutans
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Science |
Texto Completo: | https://ojs.ict.unesp.br/index.php/cob/article/view/1679 |
Resumo: | Objective: The resistance of fungi and bacteria to the available antimicrobials has increased and the development of alternative products to control them has become a very requirement. The use of plant products could be a viable option due to the efficacy, viability, and availability they present. Thereby, this study evaluated the effect of R. officinalis L. extract on C. albicans and S. mutans biofilms, by the total protein level analysis presented by the microorganisms. Material and Methods: For this purpose, monomicrobial biofilms were formed for 48 h and exposed to the R. officinalis L. extract for 5 min. Then, total protein quantification was performed by Lowry method. Results: The analysis showed significant total protein reduction of the biofilms after exposure to the plant extract, with 39 ± 11%, for C. albicans, and 32 ± 11%, for S. mutans. Conclusion: R. officinalis L. extract decreased the total protein level in both biofilms. Thus, C. albicans and S. mutans protein composition could be a target for action of antimicrobial agents.KeywordsBiofilms; Candida albicans; Proteins; Rosmarinus officinalis; Streptococcus mutans. |
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oai:ojs.pkp.sfu.ca:article/1679 |
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UNESP-20 |
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Brazilian Dental Science |
repository_id_str |
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Total protein level reduction of odontopathogens biofilms by Rosmarinus officinalis L. (rosemary) extract: an analysis on Candida albicans and Streptococcus mutansObjective: The resistance of fungi and bacteria to the available antimicrobials has increased and the development of alternative products to control them has become a very requirement. The use of plant products could be a viable option due to the efficacy, viability, and availability they present. Thereby, this study evaluated the effect of R. officinalis L. extract on C. albicans and S. mutans biofilms, by the total protein level analysis presented by the microorganisms. Material and Methods: For this purpose, monomicrobial biofilms were formed for 48 h and exposed to the R. officinalis L. extract for 5 min. Then, total protein quantification was performed by Lowry method. Results: The analysis showed significant total protein reduction of the biofilms after exposure to the plant extract, with 39 ± 11%, for C. albicans, and 32 ± 11%, for S. mutans. Conclusion: R. officinalis L. extract decreased the total protein level in both biofilms. Thus, C. albicans and S. mutans protein composition could be a target for action of antimicrobial agents.KeywordsBiofilms; Candida albicans; Proteins; Rosmarinus officinalis; Streptococcus mutans.Institute of Science and Technology of São José dos Campos2019-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documenthttps://ojs.ict.unesp.br/index.php/cob/article/view/167910.14295/bds.2019.v22i2.1679Brazilian Dental Science; Vol. 22 No. 2 (2019): Apr. - Jun. / 2019 - Published April 2019; 260-266Brazilian Dental Science; v. 22 n. 2 (2019): Apr. - Jun. / 2019 - Published April 2019; 260-2662178-6011reponame:Brazilian Dental Scienceinstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESPenghttps://ojs.ict.unesp.br/index.php/cob/article/view/1679/1357https://ojs.ict.unesp.br/index.php/cob/article/view/1679/3544https://ojs.ict.unesp.br/index.php/cob/article/view/1679/3545Copyright (c) 2019 Brazilian Dental Scienceinfo:eu-repo/semantics/openAccessde Oliveira, Jonatas RafaelSantana-Melo, Gabriela de FátimaCamargo, Samira Esteves Afonsode Vasconcellos, Luana Marotta Reisde Oliveira, Luciane Dias2020-01-28T11:57:54Zoai:ojs.pkp.sfu.ca:article/1679Revistahttp://bds.ict.unesp.br/PUBhttp://ojs.fosjc.unesp.br/index.php/index/oaisergio@fosjc.unesp.br||sergio@fosjc.unesp.br2178-60112178-6011opendoar:2022-11-08T16:30:19.949062Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)true |
dc.title.none.fl_str_mv |
Total protein level reduction of odontopathogens biofilms by Rosmarinus officinalis L. (rosemary) extract: an analysis on Candida albicans and Streptococcus mutans |
title |
Total protein level reduction of odontopathogens biofilms by Rosmarinus officinalis L. (rosemary) extract: an analysis on Candida albicans and Streptococcus mutans |
spellingShingle |
Total protein level reduction of odontopathogens biofilms by Rosmarinus officinalis L. (rosemary) extract: an analysis on Candida albicans and Streptococcus mutans de Oliveira, Jonatas Rafael |
title_short |
Total protein level reduction of odontopathogens biofilms by Rosmarinus officinalis L. (rosemary) extract: an analysis on Candida albicans and Streptococcus mutans |
title_full |
Total protein level reduction of odontopathogens biofilms by Rosmarinus officinalis L. (rosemary) extract: an analysis on Candida albicans and Streptococcus mutans |
title_fullStr |
Total protein level reduction of odontopathogens biofilms by Rosmarinus officinalis L. (rosemary) extract: an analysis on Candida albicans and Streptococcus mutans |
title_full_unstemmed |
Total protein level reduction of odontopathogens biofilms by Rosmarinus officinalis L. (rosemary) extract: an analysis on Candida albicans and Streptococcus mutans |
title_sort |
Total protein level reduction of odontopathogens biofilms by Rosmarinus officinalis L. (rosemary) extract: an analysis on Candida albicans and Streptococcus mutans |
author |
de Oliveira, Jonatas Rafael |
author_facet |
de Oliveira, Jonatas Rafael Santana-Melo, Gabriela de Fátima Camargo, Samira Esteves Afonso de Vasconcellos, Luana Marotta Reis de Oliveira, Luciane Dias |
author_role |
author |
author2 |
Santana-Melo, Gabriela de Fátima Camargo, Samira Esteves Afonso de Vasconcellos, Luana Marotta Reis de Oliveira, Luciane Dias |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
de Oliveira, Jonatas Rafael Santana-Melo, Gabriela de Fátima Camargo, Samira Esteves Afonso de Vasconcellos, Luana Marotta Reis de Oliveira, Luciane Dias |
description |
Objective: The resistance of fungi and bacteria to the available antimicrobials has increased and the development of alternative products to control them has become a very requirement. The use of plant products could be a viable option due to the efficacy, viability, and availability they present. Thereby, this study evaluated the effect of R. officinalis L. extract on C. albicans and S. mutans biofilms, by the total protein level analysis presented by the microorganisms. Material and Methods: For this purpose, monomicrobial biofilms were formed for 48 h and exposed to the R. officinalis L. extract for 5 min. Then, total protein quantification was performed by Lowry method. Results: The analysis showed significant total protein reduction of the biofilms after exposure to the plant extract, with 39 ± 11%, for C. albicans, and 32 ± 11%, for S. mutans. Conclusion: R. officinalis L. extract decreased the total protein level in both biofilms. Thus, C. albicans and S. mutans protein composition could be a target for action of antimicrobial agents.KeywordsBiofilms; Candida albicans; Proteins; Rosmarinus officinalis; Streptococcus mutans. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/1679 10.14295/bds.2019.v22i2.1679 |
url |
https://ojs.ict.unesp.br/index.php/cob/article/view/1679 |
identifier_str_mv |
10.14295/bds.2019.v22i2.1679 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/1679/1357 https://ojs.ict.unesp.br/index.php/cob/article/view/1679/3544 https://ojs.ict.unesp.br/index.php/cob/article/view/1679/3545 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Brazilian Dental Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Brazilian Dental Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document |
dc.publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
dc.source.none.fl_str_mv |
Brazilian Dental Science; Vol. 22 No. 2 (2019): Apr. - Jun. / 2019 - Published April 2019; 260-266 Brazilian Dental Science; v. 22 n. 2 (2019): Apr. - Jun. / 2019 - Published April 2019; 260-266 2178-6011 reponame:Brazilian Dental Science instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Brazilian Dental Science |
collection |
Brazilian Dental Science |
repository.name.fl_str_mv |
Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
repository.mail.fl_str_mv |
sergio@fosjc.unesp.br||sergio@fosjc.unesp.br |
_version_ |
1788346900757348352 |