Cytotoxic response of two cell lines exposed in vitro to four endodontic sealers
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian journal of oral sciences (Online) |
Texto Completo: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641437 |
Resumo: | Aim: To investigate the cytotoxicity of four endodontic sealers with different bases – Epiphany (EPH), AH Plus (AHP), Sealer 26 (S26) and Endofill (ENF) – on human foreskin fibroblasts (HFF) and mouse macrophages (J774/G8). Methods: Cells were placed in direct contact with freshly prepared endodontic sealers in polypropylene tubes. The cells were incubated for 24, 48 and 72 h. Cytotoxicity was assessed using the MTT assay (cell viability) and Griess reagent (NO release). Results: On the HFF cultures, EPH showed the lowest viability levels of all four sealers at 24 h (p<0.05), but over time (72h), EPH lessened its toxic levels in a similar pattern as the other three materials (p>0.05). The viability of all four sealers on the macrophage cultures showed no statistically significant difference over time, except between EPH and AHP at 72 h (p<0.05). Although uniformity was not detected in macrophage and fibroblast release of NO in response to sealers over time, a trend of increased NO levels for EPH (p<0.05) was observed. Conclusions: The response pattern varied depending on time and type of cell line used for analysis, although the results indicate a higher cytotoxicity for EPH in short-term tests. |
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oai:ojs.periodicos.sbu.unicamp.br:article/8641437 |
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UNICAMP-8 |
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Brazilian journal of oral sciences (Online) |
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|
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Cytotoxic response of two cell lines exposed in vitro to four endodontic sealersCell cultureCytotoxicityFibroblastMacrophageRoot canal sealersOdontologiaAim: To investigate the cytotoxicity of four endodontic sealers with different bases – Epiphany (EPH), AH Plus (AHP), Sealer 26 (S26) and Endofill (ENF) – on human foreskin fibroblasts (HFF) and mouse macrophages (J774/G8). Methods: Cells were placed in direct contact with freshly prepared endodontic sealers in polypropylene tubes. The cells were incubated for 24, 48 and 72 h. Cytotoxicity was assessed using the MTT assay (cell viability) and Griess reagent (NO release). Results: On the HFF cultures, EPH showed the lowest viability levels of all four sealers at 24 h (p<0.05), but over time (72h), EPH lessened its toxic levels in a similar pattern as the other three materials (p>0.05). The viability of all four sealers on the macrophage cultures showed no statistically significant difference over time, except between EPH and AHP at 72 h (p<0.05). Although uniformity was not detected in macrophage and fibroblast release of NO in response to sealers over time, a trend of increased NO levels for EPH (p<0.05) was observed. Conclusions: The response pattern varied depending on time and type of cell line used for analysis, although the results indicate a higher cytotoxicity for EPH in short-term tests.Universidade Estadual de Campinas2015-10-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/864143710.20396/bjos.v11i2.8641437Brazilian Journal of Oral Sciences; v. 11 n. 2 (2012): Apr./Jun.; 135-140Brazilian Journal of Oral Sciences; Vol. 11 No. 2 (2012): Apr./Jun.; 135-1401677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPporhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641437/8959Moura, Camilla Christian GomesOliveira, Natássia Cristina MartinsBorges, Cláudia Renata BibianoSouza, Maria Aparecida deBiffi, João Carlos Gabrielliinfo:eu-repo/semantics/openAccess2016-02-25T09:10:49Zoai:ojs.periodicos.sbu.unicamp.br:article/8641437Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2016-02-25T09:10:49Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false |
dc.title.none.fl_str_mv |
Cytotoxic response of two cell lines exposed in vitro to four endodontic sealers |
title |
Cytotoxic response of two cell lines exposed in vitro to four endodontic sealers |
spellingShingle |
Cytotoxic response of two cell lines exposed in vitro to four endodontic sealers Moura, Camilla Christian Gomes Cell culture Cytotoxicity Fibroblast Macrophage Root canal sealers Odontologia |
title_short |
Cytotoxic response of two cell lines exposed in vitro to four endodontic sealers |
title_full |
Cytotoxic response of two cell lines exposed in vitro to four endodontic sealers |
title_fullStr |
Cytotoxic response of two cell lines exposed in vitro to four endodontic sealers |
title_full_unstemmed |
Cytotoxic response of two cell lines exposed in vitro to four endodontic sealers |
title_sort |
Cytotoxic response of two cell lines exposed in vitro to four endodontic sealers |
author |
Moura, Camilla Christian Gomes |
author_facet |
Moura, Camilla Christian Gomes Oliveira, Natássia Cristina Martins Borges, Cláudia Renata Bibiano Souza, Maria Aparecida de Biffi, João Carlos Gabrielli |
author_role |
author |
author2 |
Oliveira, Natássia Cristina Martins Borges, Cláudia Renata Bibiano Souza, Maria Aparecida de Biffi, João Carlos Gabrielli |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Moura, Camilla Christian Gomes Oliveira, Natássia Cristina Martins Borges, Cláudia Renata Bibiano Souza, Maria Aparecida de Biffi, João Carlos Gabrielli |
dc.subject.por.fl_str_mv |
Cell culture Cytotoxicity Fibroblast Macrophage Root canal sealers Odontologia |
topic |
Cell culture Cytotoxicity Fibroblast Macrophage Root canal sealers Odontologia |
description |
Aim: To investigate the cytotoxicity of four endodontic sealers with different bases – Epiphany (EPH), AH Plus (AHP), Sealer 26 (S26) and Endofill (ENF) – on human foreskin fibroblasts (HFF) and mouse macrophages (J774/G8). Methods: Cells were placed in direct contact with freshly prepared endodontic sealers in polypropylene tubes. The cells were incubated for 24, 48 and 72 h. Cytotoxicity was assessed using the MTT assay (cell viability) and Griess reagent (NO release). Results: On the HFF cultures, EPH showed the lowest viability levels of all four sealers at 24 h (p<0.05), but over time (72h), EPH lessened its toxic levels in a similar pattern as the other three materials (p>0.05). The viability of all four sealers on the macrophage cultures showed no statistically significant difference over time, except between EPH and AHP at 72 h (p<0.05). Although uniformity was not detected in macrophage and fibroblast release of NO in response to sealers over time, a trend of increased NO levels for EPH (p<0.05) was observed. Conclusions: The response pattern varied depending on time and type of cell line used for analysis, although the results indicate a higher cytotoxicity for EPH in short-term tests. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641437 10.20396/bjos.v11i2.8641437 |
url |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641437 |
identifier_str_mv |
10.20396/bjos.v11i2.8641437 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641437/8959 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Oral Sciences; v. 11 n. 2 (2012): Apr./Jun.; 135-140 Brazilian Journal of Oral Sciences; Vol. 11 No. 2 (2012): Apr./Jun.; 135-140 1677-3225 reponame:Brazilian journal of oral sciences (Online) instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
instname_str |
Universidade Estadual de Campinas (UNICAMP) |
instacron_str |
UNICAMP |
institution |
UNICAMP |
reponame_str |
Brazilian journal of oral sciences (Online) |
collection |
Brazilian journal of oral sciences (Online) |
repository.name.fl_str_mv |
Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP) |
repository.mail.fl_str_mv |
brjorals@fop.unicamp.br||brjorals@fop.unicamp.br |
_version_ |
1788167620898324480 |