Cytotoxicity of orthodontic elastics: In vitro investigation with on L929 mouse fibroblasts

Detalhes bibliográficos
Autor(a) principal: Santos, Rogério Lacerda dos
Data de Publicação: 2015
Outros Autores: Pithon, Matheus Melo, Martins, Fernanda Otaviano, Romanos, Maria Teresa Villela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian journal of oral sciences (Online)
Texto Completo: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641794
Resumo: Aim: To test the hypothesis that there is no difference in the cytotoxicity among natural latex elastics of different manufacturers using a L929 cell line culture. Methods: Different latex intraoral elastics (I.D. = 5/16", 4.5 oz.) were tested. The sample was divided into 7 groups of 15 elastics each: Group AO (American Orthodontics), Group GAC (GAC International), Group TP (TP Orthodontics), Group AD (Aditek), Group AB (Abzil), Group MO (Morelli) and Group UN (Uniden). Cytotoxicity assays were performed by using cell culture medium containing L-929 line cells (mouse fibroblast). The cytotoxicity was evaluated by using the “dye-uptake” test, which was employed at two different moments (1 and 24 h). Data were compared by ANOVA and Tukey’s test (P < 0.05). Results: The results showed a significant difference (P < 0.05) between all groups and the group CC (cell control) at 1 and 24 h. Groups AD, AB, MO and UN were noticeably more cytotoxic than the groups AO, GAC and TP at 1 h. After 24 h, a significant decrease in cell viability was observed in all groups. Conclusions: Intraoral elastics from American Orthodontics, GAC and TP Orthodontics trademarks induced less cell lysis than Aditek, Abzil, Morelli and Uniden trademarks
id UNICAMP-8_a6a58f8217b9768ecc5fd7d8c1958c4d
oai_identifier_str oai:ojs.periodicos.sbu.unicamp.br:article/8641794
network_acronym_str UNICAMP-8
network_name_str Brazilian journal of oral sciences (Online)
repository_id_str
spelling Cytotoxicity of orthodontic elastics: In vitro investigation with on L929 mouse fibroblastsCytotoxicityElasticsBiocompatibilityOrthodonticsOdontologyAim: To test the hypothesis that there is no difference in the cytotoxicity among natural latex elastics of different manufacturers using a L929 cell line culture. Methods: Different latex intraoral elastics (I.D. = 5/16", 4.5 oz.) were tested. The sample was divided into 7 groups of 15 elastics each: Group AO (American Orthodontics), Group GAC (GAC International), Group TP (TP Orthodontics), Group AD (Aditek), Group AB (Abzil), Group MO (Morelli) and Group UN (Uniden). Cytotoxicity assays were performed by using cell culture medium containing L-929 line cells (mouse fibroblast). The cytotoxicity was evaluated by using the “dye-uptake” test, which was employed at two different moments (1 and 24 h). Data were compared by ANOVA and Tukey’s test (P < 0.05). Results: The results showed a significant difference (P < 0.05) between all groups and the group CC (cell control) at 1 and 24 h. Groups AD, AB, MO and UN were noticeably more cytotoxic than the groups AO, GAC and TP at 1 h. After 24 h, a significant decrease in cell viability was observed in all groups. Conclusions: Intraoral elastics from American Orthodontics, GAC and TP Orthodontics trademarks induced less cell lysis than Aditek, Abzil, Morelli and Uniden trademarksUniversidade Estadual de Campinas2015-11-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/864179410.20396/bjos.v9i3.8641794Brazilian Journal of Oral Sciences; v. 9 n. 3 (2010): Jul./Sep.; 366-370Brazilian Journal of Oral Sciences; Vol. 9 No. 3 (2010): Jul./Sep.; 366-3701677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPenghttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641794/9287Santos, Rogério Lacerda dosPithon, Matheus MeloMartins, Fernanda OtavianoRomanos, Maria Teresa Villelainfo:eu-repo/semantics/openAccess2016-02-25T09:12:39Zoai:ojs.periodicos.sbu.unicamp.br:article/8641794Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2016-02-25T09:12:39Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false
dc.title.none.fl_str_mv Cytotoxicity of orthodontic elastics: In vitro investigation with on L929 mouse fibroblasts
title Cytotoxicity of orthodontic elastics: In vitro investigation with on L929 mouse fibroblasts
spellingShingle Cytotoxicity of orthodontic elastics: In vitro investigation with on L929 mouse fibroblasts
Santos, Rogério Lacerda dos
Cytotoxicity
Elastics
Biocompatibility
Orthodontics
Odontology
title_short Cytotoxicity of orthodontic elastics: In vitro investigation with on L929 mouse fibroblasts
title_full Cytotoxicity of orthodontic elastics: In vitro investigation with on L929 mouse fibroblasts
title_fullStr Cytotoxicity of orthodontic elastics: In vitro investigation with on L929 mouse fibroblasts
title_full_unstemmed Cytotoxicity of orthodontic elastics: In vitro investigation with on L929 mouse fibroblasts
title_sort Cytotoxicity of orthodontic elastics: In vitro investigation with on L929 mouse fibroblasts
author Santos, Rogério Lacerda dos
author_facet Santos, Rogério Lacerda dos
Pithon, Matheus Melo
Martins, Fernanda Otaviano
Romanos, Maria Teresa Villela
author_role author
author2 Pithon, Matheus Melo
Martins, Fernanda Otaviano
Romanos, Maria Teresa Villela
author2_role author
author
author
dc.contributor.author.fl_str_mv Santos, Rogério Lacerda dos
Pithon, Matheus Melo
Martins, Fernanda Otaviano
Romanos, Maria Teresa Villela
dc.subject.por.fl_str_mv Cytotoxicity
Elastics
Biocompatibility
Orthodontics
Odontology
topic Cytotoxicity
Elastics
Biocompatibility
Orthodontics
Odontology
description Aim: To test the hypothesis that there is no difference in the cytotoxicity among natural latex elastics of different manufacturers using a L929 cell line culture. Methods: Different latex intraoral elastics (I.D. = 5/16", 4.5 oz.) were tested. The sample was divided into 7 groups of 15 elastics each: Group AO (American Orthodontics), Group GAC (GAC International), Group TP (TP Orthodontics), Group AD (Aditek), Group AB (Abzil), Group MO (Morelli) and Group UN (Uniden). Cytotoxicity assays were performed by using cell culture medium containing L-929 line cells (mouse fibroblast). The cytotoxicity was evaluated by using the “dye-uptake” test, which was employed at two different moments (1 and 24 h). Data were compared by ANOVA and Tukey’s test (P < 0.05). Results: The results showed a significant difference (P < 0.05) between all groups and the group CC (cell control) at 1 and 24 h. Groups AD, AB, MO and UN were noticeably more cytotoxic than the groups AO, GAC and TP at 1 h. After 24 h, a significant decrease in cell viability was observed in all groups. Conclusions: Intraoral elastics from American Orthodontics, GAC and TP Orthodontics trademarks induced less cell lysis than Aditek, Abzil, Morelli and Uniden trademarks
publishDate 2015
dc.date.none.fl_str_mv 2015-11-18
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641794
10.20396/bjos.v9i3.8641794
url https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641794
identifier_str_mv 10.20396/bjos.v9i3.8641794
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641794/9287
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Campinas
publisher.none.fl_str_mv Universidade Estadual de Campinas
dc.source.none.fl_str_mv Brazilian Journal of Oral Sciences; v. 9 n. 3 (2010): Jul./Sep.; 366-370
Brazilian Journal of Oral Sciences; Vol. 9 No. 3 (2010): Jul./Sep.; 366-370
1677-3225
reponame:Brazilian journal of oral sciences (Online)
instname:Universidade Estadual de Campinas (UNICAMP)
instacron:UNICAMP
instname_str Universidade Estadual de Campinas (UNICAMP)
instacron_str UNICAMP
institution UNICAMP
reponame_str Brazilian journal of oral sciences (Online)
collection Brazilian journal of oral sciences (Online)
repository.name.fl_str_mv Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)
repository.mail.fl_str_mv brjorals@fop.unicamp.br||brjorals@fop.unicamp.br
_version_ 1800216399989178368