Oral mucositis and microbial status in acute lymphoblastic leukemia subjects undergoing high-dose chemotherapy
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian journal of oral sciences (Online) |
Texto Completo: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8667697 |
Resumo: | Aim: To assess oral microbial status in patients with acute lymphoblastic leukemia (ALL) undergoing high-dose chemotherapy and to unravel possible associations between nosocomial pathogens and the establishment of chemotherapy-induced oral mucositis (CIOM). Methods: Oral mucosa, saliva, and peripheral blood samples were collected from 46 ALL subjects one day prior to chemotherapy (D0) and 2 weeks after treatment initiation (D14). Clinical intraoral inspection was performed by a single practitioner, with mucositis classification performed according to the WHO oral toxicity scale. Blood components were quantified by automatic flow cytometry, while oral Staphylococcus aureus and Pseudomonas aeruginosa were detected by Polymerase Chain Reaction with species-specific primers. Associations among bacteria and clinical findings were determined by Fisher’s Exact test, longitudinal bacterial changes by paired Macnemar, and correlations among blood parameters and mucositis status or bacteria via Mann-Whitney. Results: S. aureus displayed higher detection rates at D14 (p < 0.05) and was positively associated with mucositis, adoption of a non-solid diet (all p < 0.001), nausea and fever (all p < 0.05). Conversely, P. aeruginosa did not correlate to CIOM clinical parameters. At the systemic standpoint, lower hemoglobin levels associated with CIOM and fever events (all p < 0.01). Conclusion: The study evidences S. aureus as a potential pathogen in ALL-CIOM, reaffirming microbial control as an important preventive measure during high-dose immunosuppressive therapy. The weight of non-white-blood-cell parameters should be validated as novel CIOM biomarkers in prospective research. |
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UNICAMP-8 |
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Brazilian journal of oral sciences (Online) |
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Oral mucositis and microbial status in acute lymphoblastic leukemia subjects undergoing high-dose chemotherapyOral mucositisAntineoplastic agentsPolymerase chain reactionBacteriaAcute lymphoblastic leukemiaAim: To assess oral microbial status in patients with acute lymphoblastic leukemia (ALL) undergoing high-dose chemotherapy and to unravel possible associations between nosocomial pathogens and the establishment of chemotherapy-induced oral mucositis (CIOM). Methods: Oral mucosa, saliva, and peripheral blood samples were collected from 46 ALL subjects one day prior to chemotherapy (D0) and 2 weeks after treatment initiation (D14). Clinical intraoral inspection was performed by a single practitioner, with mucositis classification performed according to the WHO oral toxicity scale. Blood components were quantified by automatic flow cytometry, while oral Staphylococcus aureus and Pseudomonas aeruginosa were detected by Polymerase Chain Reaction with species-specific primers. Associations among bacteria and clinical findings were determined by Fisher’s Exact test, longitudinal bacterial changes by paired Macnemar, and correlations among blood parameters and mucositis status or bacteria via Mann-Whitney. Results: S. aureus displayed higher detection rates at D14 (p < 0.05) and was positively associated with mucositis, adoption of a non-solid diet (all p < 0.001), nausea and fever (all p < 0.05). Conversely, P. aeruginosa did not correlate to CIOM clinical parameters. At the systemic standpoint, lower hemoglobin levels associated with CIOM and fever events (all p < 0.01). Conclusion: The study evidences S. aureus as a potential pathogen in ALL-CIOM, reaffirming microbial control as an important preventive measure during high-dose immunosuppressive therapy. The weight of non-white-blood-cell parameters should be validated as novel CIOM biomarkers in prospective research.Universidade Estadual de Campinas2023-04-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/otherapplication/pdfhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/866769710.20396/bjos.v22i00.8667697Brazilian Journal of Oral Sciences; v. 22 (2023): Continuous Publication; e237697Brazilian Journal of Oral Sciences; Vol. 22 (2023): Continuous Publication; e2376971677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPenghttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8667697/31770Brazil; ContemporaryCopyright (c) 2022 Ana Cláudia Nunes Duarte, Anderson Nogueira Barbosa, Cristiane Pereira Borges Saito, Erich Vinicius de Paula, Daniel Saitohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessNunes Duarte, Ana CláudiaBarbosa, Anderson Nogueira Saito, Cristiane Pereira Borges Paula, Erich Vinicius deSaito, Daniel2023-04-05T13:19:27Zoai:ojs.periodicos.sbu.unicamp.br:article/8667697Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2023-04-05T13:19:27Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false |
dc.title.none.fl_str_mv |
Oral mucositis and microbial status in acute lymphoblastic leukemia subjects undergoing high-dose chemotherapy |
title |
Oral mucositis and microbial status in acute lymphoblastic leukemia subjects undergoing high-dose chemotherapy |
spellingShingle |
Oral mucositis and microbial status in acute lymphoblastic leukemia subjects undergoing high-dose chemotherapy Nunes Duarte, Ana Cláudia Oral mucositis Antineoplastic agents Polymerase chain reaction Bacteria Acute lymphoblastic leukemia |
title_short |
Oral mucositis and microbial status in acute lymphoblastic leukemia subjects undergoing high-dose chemotherapy |
title_full |
Oral mucositis and microbial status in acute lymphoblastic leukemia subjects undergoing high-dose chemotherapy |
title_fullStr |
Oral mucositis and microbial status in acute lymphoblastic leukemia subjects undergoing high-dose chemotherapy |
title_full_unstemmed |
Oral mucositis and microbial status in acute lymphoblastic leukemia subjects undergoing high-dose chemotherapy |
title_sort |
Oral mucositis and microbial status in acute lymphoblastic leukemia subjects undergoing high-dose chemotherapy |
author |
Nunes Duarte, Ana Cláudia |
author_facet |
Nunes Duarte, Ana Cláudia Barbosa, Anderson Nogueira Saito, Cristiane Pereira Borges Paula, Erich Vinicius de Saito, Daniel |
author_role |
author |
author2 |
Barbosa, Anderson Nogueira Saito, Cristiane Pereira Borges Paula, Erich Vinicius de Saito, Daniel |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Nunes Duarte, Ana Cláudia Barbosa, Anderson Nogueira Saito, Cristiane Pereira Borges Paula, Erich Vinicius de Saito, Daniel |
dc.subject.por.fl_str_mv |
Oral mucositis Antineoplastic agents Polymerase chain reaction Bacteria Acute lymphoblastic leukemia |
topic |
Oral mucositis Antineoplastic agents Polymerase chain reaction Bacteria Acute lymphoblastic leukemia |
description |
Aim: To assess oral microbial status in patients with acute lymphoblastic leukemia (ALL) undergoing high-dose chemotherapy and to unravel possible associations between nosocomial pathogens and the establishment of chemotherapy-induced oral mucositis (CIOM). Methods: Oral mucosa, saliva, and peripheral blood samples were collected from 46 ALL subjects one day prior to chemotherapy (D0) and 2 weeks after treatment initiation (D14). Clinical intraoral inspection was performed by a single practitioner, with mucositis classification performed according to the WHO oral toxicity scale. Blood components were quantified by automatic flow cytometry, while oral Staphylococcus aureus and Pseudomonas aeruginosa were detected by Polymerase Chain Reaction with species-specific primers. Associations among bacteria and clinical findings were determined by Fisher’s Exact test, longitudinal bacterial changes by paired Macnemar, and correlations among blood parameters and mucositis status or bacteria via Mann-Whitney. Results: S. aureus displayed higher detection rates at D14 (p < 0.05) and was positively associated with mucositis, adoption of a non-solid diet (all p < 0.001), nausea and fever (all p < 0.05). Conversely, P. aeruginosa did not correlate to CIOM clinical parameters. At the systemic standpoint, lower hemoglobin levels associated with CIOM and fever events (all p < 0.01). Conclusion: The study evidences S. aureus as a potential pathogen in ALL-CIOM, reaffirming microbial control as an important preventive measure during high-dose immunosuppressive therapy. The weight of non-white-blood-cell parameters should be validated as novel CIOM biomarkers in prospective research. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/other |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8667697 10.20396/bjos.v22i00.8667697 |
url |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8667697 |
identifier_str_mv |
10.20396/bjos.v22i00.8667697 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8667697/31770 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Oral Sciences; v. 22 (2023): Continuous Publication; e237697 Brazilian Journal of Oral Sciences; Vol. 22 (2023): Continuous Publication; e237697 1677-3225 reponame:Brazilian journal of oral sciences (Online) instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
instname_str |
Universidade Estadual de Campinas (UNICAMP) |
instacron_str |
UNICAMP |
institution |
UNICAMP |
reponame_str |
Brazilian journal of oral sciences (Online) |
collection |
Brazilian journal of oral sciences (Online) |
repository.name.fl_str_mv |
Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP) |
repository.mail.fl_str_mv |
brjorals@fop.unicamp.br||brjorals@fop.unicamp.br |
_version_ |
1788167625073754112 |